Bruker Fourier 300, Быстрое руководство по установке

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15 Z33048_00_01
Getting Started
• Lineshape test for 1H with rotation
• Resolution test for 1H
• Sensitivity test for 1H
• Lineshape Test for 13C with rotation
• Resolution test for 13C
• Sensitivity test for 13C (ASTM)
• Sensitivity test for 13C (EB)
• HSQC experiment with Z?gradient (console test)
• COSY experiment with Z?gradient (console test)
• DEPT?90 experiment
• DEPT?135 experiment
There are only three hardware specifications for the Fourier required for a customer sign
off:
Lineshape using 3% CDCl3 in Acetone: 0.5/8/16 Hz (non-spinning)
1H Sensitivity (0.1% EB in CDCL3):
> 120:1 (after gradshim)
C13 Sensitivity (ASTM): > 85:1 (after gradshim).
The remaining ATP tests implicitly demonstrate the systems remaining functions:
•
Sample Spin & Lift
• Auto-Locking on various solvents
• Gradient Functionality (2Ds & Gradshim)
• Gradshim reproducibility
• Lock and Temperature Stability (COSY & HSCQ)
• Baseline & Spectral Purity (DEPT)*
Certain artefacts are normal and have been considered acceptable for the intended use
of the Fourier:
•
A moderate amount of T1 noise in 2D experiments.
• Some “digital noise” on strong signals that require RG < 10. Use
zg30
instead of
zg
if that happens, in order to be able to increase the RG.
• Some vibration noise close to the feet of very narrow lines (e.g. lineshape samples).
This can be improved with vibration damping if required.
• Some very small spurious spikes in the first few scans of C13 spectra. These should
average out after NS > 64.
• “Asymmetric smileys” in some 1D spectra with high intensity. This can also be seen
as reduction in the level of noise of about 200 Hz from the right edge of some 1D