CoolBox
TM
2XT
Instructions for Use
Overview of CoolBox
™
2XT System
CoolBox 2XT is designed for maintaining sample temperatures below 4°C on the bench top with-
out the use of wet ice or electricity. Patent-pending dual-phase conductive XT Cooling Core or XT
Freezing Core provides the cooling source when a thermo-conductive CoolRack
®
or CoolSink
®
tube
or plate module is placed on top. The core and sample module in combination ensure uniform
well-to-well temperature throughout the cooling period regardless of sample position. XT Cooling
Core can maintain sample temperature from 0.5 to 4°C for over 16 hours; XT Freezing Core can
maintain frozen samples for 10 hours; using dry ice in the base provides an ultra-cold temperature
(-78°C) for snap-freezing samples in tubes or plates. For a list of CoolRack and CoolSink modules
that are compatible with the CoolBox 2XT, see back page.
Assembly of CoolBox
™
2XT
The CoolBox 2XT system consists of a closed-cell, cross-linked polyethylene foam base, collar, lid
and a reusable XT Cooling Core. A CoolBox 2XT also includes a CoolRack
®
or CoolSink
®
tube or
plate module. When placed on top of the XT Cooling or Freezing Core, the modules and samples
rapidly equilibrate to the temperature of the XT Core. Samples can be placed in the CoolRack
or CoolSink module either before or after the module is placed in CoolBox XT. When used with
CoolSink plate modules the magnetized collar is not required and can be removed for easy access
when working; however, to obtain the maximum cooling duration, we recommend using the collar
whenever possible.
Quick Start
• Remove two XT Cooling Cores from -20°C
freezer and place on bench.
• When temperature strip on XT Cooling
Core registers 1°C, place CoolRack
®
or
CoolSink
®
sample modules in the Cool-
Box
™
XT base and place collar on top.
• Load samples in CoolRack or CoolSink
modules.
Caution
• Lift CoolBox 2XT from the bottom using
the handholds on the sides.
• Always use two hands when carrying or
lifting CoolBox 2XT.
• Avoid touching the top metal surface of
the XT Cooling Core and XT Freezing Core
when removing from freezer.
IMPORTANT: To ensure your samples do not freeze and to get maximum cooling duration, please
refer to the detailed instructions that follow.
*All tests were performed using a CoolRack
®
XT M24 loaded with 24 TruCool
®
2.0 mL microcentrifuge
tubes filled with 1.5 mL water. Actual performance may vary depending upon CoolRack
®
module em-
ployed, sample load, initial sample temperature, ambient temperature, air currents, and other conditions.
Working
Temperature Range
CoolBox XT
Cooling Source
Temperature Duration
*
Open Lid
Temperature Duration
*
Closed Lid
0.5° to 4
o
C
XT Cooling Core
Over 10 hours
Over 16 hours
-20° to 0
o
C
XT Freezing Core
Over 5 hours
Over 8 hours
-78
o
C
200 ml Dry Ice
Over 4 hours
Over 5 hours
CoolRack
®
and CoolSink
®
Thermo-Conductive Sample Modules Compatible with CoolBox XT
Item
Description
Accommodates
BCS-163
CoolRack M6
6 x 1.5 mL or 2.0 mL microfuge tubes
BCS-125
CoolRack M15
15 x 1.5 mL or 2.0 mL microfuge tubes
BCS-127
CoolRack M15-PF
15 x 1.5 mL conical microfuge tubes
BCS-535
CoolRack XT M24
24 x 1.5 mL or 2.0 mL microcentrifuge tubes, SBS-compliant
BCS-126
CoolRack CF15
15 x 1.0 mL or 2.0 mL cryogenic vials
BCS-534
CoolRack XT CFT24
24 cryogenic vials, with “gripping” wells for one-hand vial open/close, SBS-compliant
BCS-529
CoolRack XT PCR96
PCR tubes, strips or 96-well plate
BCS-523
CoolRack XT M-PCR
12 x 1.5 mL or 2.0 mL microcentrifuge tubes and 6 PCR strip wells
BCS-538
CoolRack XT PCR384
one 384-well PCR plate, SBS-compliant
BCS-231
CoolRack 96x0.5ml
96 x 0.5 mL 2D barcode tubes
BCS-149
CoolRack 96x1ml
96 x 1.0 mL 2D barcode tubes
BCS-536
CoolSink XT 96F
one flat-bottom 96- or 384-well assay plate, SBS-compliant
BCS-537
CoolSink XT 96U
one u-bottom 96-well assay plate, SBS-compliant
Tall Tube Modules - requires Extension Collar, if lid closing is necessary
BCS-232
CoolRack L
12 x 15 mL centrifuge tubes
BCS-153
CoolRack 15ml
9 x 15 mL centrifuge tubes
BCS-154
CoolRack 50ml
4 x 50 mL centrifuge tubes
BCS-235
CoolRack LV
12 x 13mm or 16mm diameter tubes
BCS-157
CoolRack VS13
9 x 13x75mm blood tubes
BCS-155
CoolRack V13
9 x 13x100mm blood tubes or 5.0 mL cryogenic vials
BCS-156
CoolRack VS16
9 x 16x100mm blood tubes
CAUTION: The products described here are intended for the exclusive use by trained and experienced
laboratory and medical personnel. Use of dry ice can be dangerous. Direct skin contact with dry ice or
metal components that have been in contact with dry ice can cause freezing injury. Always use appropri-
ate protective equipment for eyes and skin when handling dry ice and cold metal components.
Made in USA.
15 Elizabeth Dr., Chelmsford, MA 01824 USA brookslifesciences.com/contact-us brookslifesciences.com
CoolBox 2XT Instructions, May 2018
CoolBox 2XT ©Brooks Automation, Inc. All rights reserved.
