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25

Pipetting

5.2

In the case of blow-out pipettes

(Imprint 'ausblasen – blow out'):

– As soon as the meniscus in the pipette tip

comes to a standstill, press the small blow-
out bellows once.

– Wipe the pipette tip a few millimeters

upward along the wall of the vessel.

6.

After pipetting

Hold the pipette as its extreme upper end,
and gently twist and pull it out of the adapter.

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Содержание macro

Страница 1: ...macro Manual de opera o Operating Manual...

Страница 2: ......

Страница 3: ...Pipetagem 7 Limpeza Autoclava o 10 Substitui o do Filtro e Limpeza do Adaptador 10 Substitui o do Sistema de V lvulas 12 Teste de Vazamento 13 Informa es para pedido Pe as de Reposi o 14 Resolu o de...

Страница 4: ...te dos reagentes utilizados 4 Utilize o instrumento somente para pipetar l quidos respeitando rigorosamente as limita es de uso Observe as opera es n o reco mendadas veja p g 5 Em caso de d vida conta...

Страница 5: ...trumento indicado para pipetar l quidos observando as seguintes limita es f sicas de 10 C a 40 C de 50 F a 104 F instrumento e reagente Press o de vapor at 500 mbar Aspirar lentamente acima de 300 mba...

Страница 6: ...leno PTFE Politetrafluoretileno SI Borracha de silicone 6 Comando de pipetagem Pera de suc o SI Filtro membrana PP PTFE Adaptador SI Suporte do adaptador PP Anel de trava PP Bot o de sopro SI Carca a...

Страница 7: ...Se o l quido entrar no instrumento substitua o filtro veja p g 10 11 Aten o 1 Comprima a pera de suc o Antes de conectar a pipeta aperte a pera de suc o 2 Conecte a pipeta Segure a pipeta o mais pr xi...

Страница 8: ...s rio Pressione o comando de pipetagem para baixo lenta mente at que um ajuste preciso do menico seja atingido 5 Dispensar o l quido da pipeta Segure o recipiente coletor em uma posi o inclinada Posic...

Страница 9: ...ficar em uma posi o est vel aperte o bot o pe quena pera de borracha blow out uma vez Arraste a ponta da pipeta alguns mil metros para cima na parede do recipiente para retirar a gota remanescente 6...

Страница 10: ...o Somente necess rio se aspirar al m do limite da pipeta ou se a capacidade de suc o estiver prejuducada Substitui o do Filtro e Limpeza do Adaptador Use luvas e culos de prote o Evite o risco de con...

Страница 11: ...baixo no adapta dor com o lado c nico menor para cima Nem todo filtro membrana adequado Utilize somente acess rios recomendados pelo fabricante Filtros de tamanho de poro 3 m s o o padr o para o instr...

Страница 12: ...do Sistema de V lvulas 1 Desrosqueie a pera de suc o e o supor te do adaptador 2 Retire a parte superior da carca a Puxe com cuidado o sistema de valvulas e substitua o 3 Monte o equipamento na ordem...

Страница 13: ...tugu s 13 Limpeza Autoclava o Quando o menisco estiver ajustado o l quido n o pode gotejar antes que as v lvulas sejam ativadas Se ocorrer gotejamento veja Resolu o de Problemas p g 15 Teste de vazame...

Страница 14: ...tema de v lvulas Ref 261 28 Macro controlador de pipetagem Com filtro membrana 3 m sobressalente e manual de opera es As cores se referem as cores no suporte adaptador do instrumento e no anel de trav...

Страница 15: ...te adaptador do instrumen to Substitua o adaptador de silicone Pera de suc o enche de ar auto maticamente ap s compress o Pera de suc o ou sistema de v lvulas danificados Substitua o anel de trava com...

Страница 16: ...bricante ou fornecedor Solicite o modelo ao seu fornecedor ou fabricante O modelo tamb m se encontra na p gina www brand de para download Retorno para reparo Importante Transporte de produtos perigoso...

Страница 17: ...0 767 2562 Fax 1 860 767 2563 www brandtech com India BRAND Scientific Equipment Pvt Ltd 303 3rd Floor C Wing Delphi Hiranandani Business Park Powai Mumbai 400 076 India Tel 91 22 42957790 Fax 91 22 4...

Страница 18: ...u consequ ncias do desgaste normal especialmente de pe as suscet veis a desgaste como pist es selos v lvulas e quebra de vidro assim como do descumprimento das instru es contidas neste manual N o nos...

Страница 19: ...1 Operating Elements 22 Pipetting 23 Cleaning Autoclaving 26 Replacing the Filter and Cleaning the Adapter 26 Replacing the Valve System 28 Leak Test 29 Ordering Data Spare Parts 30 Troubleshooting 31...

Страница 20: ...vided by reagent manufacturers 4 Use the instrument only for pipetting liquids with strict regard to the defined limitations of use Observe operating exclusions s page 21 If in doubt contact the manuf...

Страница 21: ...r pipetting liquids observing the following physical limits from 10 C to 40 C from 50 F to 104 F instrument and reagent Vapor pressure up to max 500 mbar Aspirate slowly above 300 mbar in order to pre...

Страница 22: ...opylene PTFE Polytetrafluorethylene SI Silicone rubber 22 pipetting lever suction bellows SI membrane filter PP PTFE adapter SI adapter support PP locking ring PP blow out bellows SI housing PP valve...

Страница 23: ...does enter the instrument exchange the filter see pages 26 27 Attention 1 Compress the suction bellows Before attaching the pipette squeeze the suction bellows 2 Attach the pipette Hold the pipette as...

Страница 24: ...ssue to wipe the pipette tip if necessary Press the pipetting lever down slowly until precise adjustment of the meniscus has been achieved 5 Dispense the pipette Hold the collecting vessel in an incli...

Страница 25: ...As soon as the meniscus in the pipette tip comes to a standstill press the small blow out bellows once Wipe the pipette tip a few millimeters upward along the wall of the vessel 6 After pipetting Hold...

Страница 26: ...leaning Autoclaving Only necessary if a pipette has been overfilled or the suction capacity is impaired Replacing the Filter and Cleaning the Adapter Wear protective gloves and eye protection Avoid th...

Страница 27: ...wnwards into the adapter with the smaller conical side up Not every membrane filter is suitable Only use genuine manufacturer s recommended accessories Filters of a pore size of 3 m are a standard equ...

Страница 28: ...e the Valve System 1 Unscrew the suction bellows and the adapter support 2 Take off the upper part of the housing Carefully pull out the valve system and replace it 3 Reassemble the instrument in reve...

Страница 29: ...sh 29 Cleaning Autoclaving When the meniscus has been set liquid should not drip out of the pipette until the valves are activated Should the pipette drip see chapter Troubleshooting see page 31 Leak...

Страница 30: ...Cat No 261 28 macro pipette controller with spare membrane filter 3 m and operating manual Colors refer to accent colors on instrument adapter support and locking ring Color Cat No grey 262 00 green 2...

Страница 31: ...the instru ment replace silicone adapter Compressed suction bellows take in air automatically Suction bellows or valve system damaged Replace the looking ring with the suction bellows or the valve sys...

Страница 32: ...strument to the manufacturer or supplier Ask your supplier or manufac turer for the form The form can also be downloaded from www brand de Return for repair Important Transporting of hazardous materia...

Страница 33: ...767 2562 Fax 1 860 767 2563 www brandtech com India BRAND Scientific Equipment Pvt Ltd 303 3rd Floor C Wing Delphi Hiranandani Business Park Powai Mumbai 400 076 India Tel 91 22 42957790 Fax 91 22 42...

Страница 34: ...onsequences of normal wear and tear especially of wearing parts such as pistons seals valves and the breakage of glass as well as the failure to follow the instructions of the operating manual We are...

Страница 35: ......

Страница 36: ...9976 40 Printed in Germany 21 0620 7...

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