
diaphragm image, remove the eyepiece and look down the
open tube at the exit pupil of the objective.
8. Field Diaphragm Adjustment
(For the field lens unit with Field Diaphragm)
Stop down the field diaphragm until the illuminated area
closely circumscribes the eyepiece viewfield.
The field diaphragm is used to control the specimen’s
illuminated area relative to the microscope viewfield. If it is
opened to a larger aperture than necessary, extraneous light
will enter the viewfield, reducing image quality and contrast.
9. Oil-immersion Observation
When using the 100X objectives, it is an oil-immersion type
requiring the application of immersion oil between its lens tip
and the coverglass.
If air bubbles enter the oil layer, a poor image will result. To
remove the air bubbles, rotate the nosepiece several times, or
apply an additional drop of oil.
After completing oil-immersion observation, be sure to clean
the front lens of the objective and any other parts coated with
oil.
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