Biotek Synergy LX, Руководство пользователя

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81 | Chapter 5 : Instrument Qualification Process
Luminescence Test
For models with luminescence capability, BioTek uses the Harta Luminometer Reference
Microplate to test the luminescence system. The test plate is LED-based and NIST-
traceable. Contact BioTek to purchase a plate (BTI #8030015; includes microplate carrier
adapters) or visit www.HartaInstruments.com to learn more.
Test Method
The Harta Luminometer Reference Microplate is used to determine a detection limit by
leveraging a known correlation of 35 photons per attomole of ATP. By using the NIST data
provided with the Harta plate in photons/s, a conversion factor of 0.02884
attomole/photon is applied to determine an ATP concentration and subsequent limit of
detection for the instrument under test.
Plate Layout
Results Analysis
The Luminescence Test procedure is described on page 106.
1. Determine if the plate’s battery is functioning properly:
o
If A8 > (0.2 * A7), the battery is good. Otherwise, it requires replacement.
A replacement battery is included with each Harta plate. A new spare battery will
be supplied when the plate is recertified.
Synergy LX User Manual
1501000N Revision A BioTek Instruments, Inc.