Biotek Synergy H1, Руководство оператора

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Alignment: The test plate has precisely machined holes to confirm mechanical
alignment. The amount of light that shines through these holes is an indication of
whether the microplate carrier is properly aligned with the absorbance optical path.
A reading of more than 0.015 OD for any of the designated alignment holes indicates
that the light is being “clipped” and the reader may be out of alignment.
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Accuracy: The test plate contains NIST-traceable neutral-density glass filters of
known OD values at one or more wavelengths. Actual measurements are compared
against the expected values provided in the test plate’s data sheet. Since there are
several filters with differing OD values, the accuracy across a range of ODs can be
established. Once it is proven that the reader is accurate at these OD values, the
reader is also considered to be linear. To further verify this, you can perform a linear
regression analysis on the test plate OD values in a program such as Microsoft Excel;
an R
2
value of at least 0.9900 is expected.
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Repeatability: This test ensures the instrument meets its repeatability specification
by conducting repeated reads of each neutral-density filter on the test plate and
comparing the results.
Sample Test Report
Refer to Sample Reports on page 155 to see a sample Absorbance Plate Test Report for
Synergy H1.
Troubleshooting
If a test fails, try the troubleshooting tips below. If the test continues to fail, contact
BioTek TAC.
Important! Do not remove filters from the Absorbance Test Plate.
Do not use alcohol or other cleaning agents, and do not touch the
filters with your bare fingers.
If a higher-OD well reports "#N/A" for Min/Max Limit and Result,
the measured OD is beyond the specified range for Accuracy or
Repeatability used with this test, and therefore no pass/fail
determination is made. It does not indicate a test failure.
Peak Absorbance Test
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Check the filter in the C6 position to ensure it is clean. If needed, clean the filter with
lens paper. Do not remove the filter, and do not use alcohol or other cleaning agents.
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Verify that the Peak wavelength information entered for the plate in Gen5 matches
the information provided on the test plate's data sheet.
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Check the calibration due date on the test plate's label. If the test plate is overdue for
76 | Chapter 7: Instrument Qualification Process
BioTek Instruments, Inc.