Biotek MultiFlo FX, Руководство оператора

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Washer Qualification Liquid Tests | 253
Parameters 6-Well 12-Well 24-Well 48-Well 384-well
Flow Rate 5 5 5 5 5
Z-axis/Dispense
Height 465 460 452 460 333
Pre-dispense
For all manifold types enable and set its
Volume
to 300 µL and
Number
of cycles to 2 .
Retain default values for all other parameters.
Evacuation Efficiency Protocols
Parameters 6-Well 12-Well 24-Well 48-Well
Travel Rate 0 1 1 1
Z-axis 45 51 51 42
X-axis 350 222 150 96
Retain default values for all other parameters.
Dispense Precision and Accuracy Test
n
Find a list of the supplies you will need at Materials on page 251 .
n
Save the plate! When this test is complete, use the filled plate to perform the
Evacuation Efficiency Test.
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Prerequisite for plate types other than 96-Well : Create the protocols
needed to test your manifold. See Create Washer Qualification Protocols
on the previous page . One time only! (Predefined protocols are provided
with the instrument qualification package (PN 1260521).
1. Fill a supply bottle with 2 liters of deionized or distilled water and run the
W-
DAY_RINSE
protocol once or twice to flush the fluid lines and manifold.
2. Fill a supply bottle with 1200 mL of Solution #3 .
3. Run
W-DAY_RINSE
again to prime the washer with the solution.
4. Place a new microplate on the balance and zero the balance.
5. Place the plate on the carrier and run the
QC_96_DISP_TEST
or the protocol
you created for your manifold. The protocol dispenses solution to each well of
the plate. It does not evacuate the solution.
BioTek Instruments, Inc.