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SPE Dry 96 and SPE Dry 96 Dual User Manual | © Biotage 2017
Maintenance
The System
Maintaining the cleanliness of your work area and of your
system will help ensure that your system operates efficiently.
Simply wipe down the system regularly with a clean cloth lightly
dampened with a mild solvent or cleanser.
Inspect the system regularly for worn components and replace
them immediately. The gas line and hose clamps should be
inspected prior to each use. Confirm that they are in good
condition to prevent the hose from bursting or blowing off
the system.
Removing, inspecting, and cleaning the needle assemblies
should be part of a regular maintenance program. To remove
the upper needle assembly, simply depress the latches located
on each side of the head and the needle assembly will slide
straight out for easy cleaning.
Inspect and lubricate the lower head sliding mechanism
regularly with system-grade lubricant.
Figure 6
Slide out the
needle assembly
Catch
Latch
.
The upper needle assembly of the SPE Dry 96 system.
Figure 7
Slide out the
needle assembly
Latch
Catch
.
The upper needle assembly of the SPE Dry 96 Dual system.
Suggested Wash Procedure for the Needles
The needles are 304 stainless steel and the head assembly is
constructed of acetal-copolymer. It is highly recommended that
an appropriate daily cleaning procedure be devised so that the
needle assemblies do not become contaminated or attacked by
harsh solvent.
There are a number of ways to clean the needle assemblies.
The two most common methods are submersion and sonication.
Submersion
1. Submerse the needles in 60% methanol/40% deionized
water for a few minutes with unheated gas flowing slowly
through the needle assembly. Agitation from the bubbles
will usually clean and lift off what may be left from the
previous sample as contamination carry-over.
2. Rinse with 100% methanol.
3. Repeat as necessary for heavily contaminated needles.
4. Remove the cleaning reagent and allow gas to flow
(unheated) until dry.
Sonication
1. Remove the head by depressing the latches located on each
side of the head; see Figure 6 and Figure 7.
2. Fill the sonicator with 60% methanol/40% deionized water.
3. Position the needle assembly in the sonicator as shown in
Figure 8.
Figure 8.
The correct position of the needle assembly in the sonicator.
4. Sonicate the needle assembly for 30 minutes. This provides
the highest level of protection against sample carry-over.
5. Rinse with 100% methanol.
6. Allow head to air dry. Alternatively, it may be placed back
onto the system and dried utilizing unheated gas.
Maintenance