Biotage Selekt Enkel, Руководство пользователя

Страница: 34 / 44

32 Biotage
®
Selekt Enkel User Manual | © Biotage 2022
Maintenance
3. Remove the check valve by pushing an unused pipette tip
or similar into the check valve and then pulling it out; see
Figure 60.
4. Push on the ball inside the check valve using an unused
pipette tip or similar; see Figure 61. Otherwise, rinse the
check vale with IPA or methanol, especially if halogenated
solvent (e.g. DCM) is used with the system, or replace the
check valve (P/N 417440SP).
5. Repeat the procedure for the other three check valves.
Figure 60. Removing the check valve.
Figure 61. Release a stuck check valve.
Change Between Normal and Reversed Phase
To avoid issues during a solvent change, it is necessary to
perform the change throughout the entire chromatographic
system (i.e. in the reservoir, pump, tubing, and detector).
The procedure is to flush the system (see page 20), with
a series of mutually miscible solvents until a gradual change
to the new solvent is accomplished. If this procedure is not
followed, precipitation may occur not only in the flow cell of the
UV detector but also in other parts of the system. For example,
to change from organic solvents to aqueous solutions, it is
necessary to flush the whole system with acetone or an alcohol.
Leaks
Warning
»
Always handle leakage immediately.
»
Follow all generally-accepted lab safety procedures and
applicable laws and regulations.
»
Always follow local and national safety regulations and the
solvent manufacturer’s safety, handling, storage, and disposal
recommendations; refer to the safety data sheets (SDS).
»
Electrical equipment can introduce ignition hazards. Ensure that
all solvent manufacturers’ recommendations are followed with
respect to handling, ventilation, and operating environment.
»
Personnel working with or near the system must wear protective
clothing, safety gear, and eye protection in accordance with
applicable local and national safety regulations.
»
Shut down the system before replacing any tubing. Use only
tubing designed for the Selekt Enkel system and supplied by
Biotage.
Shut Down the System at Leakage
If a leakage is observed, shut down the system as follows:
1. If a purification is in progress, press and then Abort .
2. If a prime or flush is in progress (started by the user),
press Stop .
3. Press Menu , Shut Down , and then Yes to confirm.
4. When the message saying it is safe to turn off the system
appears on the touch screen, turn off the system. The power
switch is located on the left side of the system.
External Leakage
External leakage may occur due to e.g. loose fittings or
damaged tubing. Any leakage in the flow cell of the UV detector
is drained via the drip sheet; see Figure 62.
Figure 62. Drainage of leakage from the flow cell of the UV detector.
All external tubing on the system except for the tubing on the
collection arm can be replaced by the user; a list of spare parts
is available at www.biotage.com.
Note:
All tube types, dimensions, and lengths are essential
for the performance of the system. Only replace tubes with the
equivalent tubes designed for the Selekt Enkel system and
supplied by Biotage.