Biotage Isolera Four, Руководство пользователя

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411829-L, Maintenance April 2012 Page 5-4
5
To clean the detector flow cell:
1. Shut down the system as described in on page 4-1.
2.
While placing one hand under the flow cell (to prevent it from falling), pull outward on the
retaining latch to unlock the flow cell, and then carefully remove the flow cell from the system.
NOTE
The retaining latch should close with
little effort. If the retaining latch is
difficult to close, the flow cell is probably
misaligned.
Figure 5-1. Remove the Detector Flow Cell (Isolera Prime, One, and Four Shown)
3. Disconnect the inlet and outlet tubing from the flow cell and visually inspect the cell for
contamination.
4. Flush the flow cell with a series of miscible solvents. Select the solvents based on the
contamination. It is possible to use both organic and inorganic solvents and diluted solutions of
acids (e.g. H
2
SO
4
or HNO
3
diluted with distilled water in a ratio of 1:20 to 1:10), unless they
attack stainless steel, PTFE, or fused silica windows.
5. Visually re-examine cell windows for visible contamination. If contamination is still present,
repeat step 4. If you are not able to remove the contamination, replace the flow cell (P/N 09869
for Isolera Prime. One, and Four systems and P/N 412324 for Isolera LS systems) and perform
an absorbance calibration (see “Calibrate the Internal Detector” on page 3-13).
6. Reconnect the tubing to the flow cell.
7. Carefully insert the flow cell and close the retaining latch.
5.7 Clean or Release Valves
5.7.1 Clean the Pump Check Valves
If the last purification of the day is performed with a halogenated solvent (e.g. DCM), we
recommend that you flush the pump check valves with methanol or a similar solvent. Use the
following procedure to flush the pump check valves.
1. Place the solvent inlet line used with the halogenated solvent into methanol or a similar solvent.
2. Ensure that the waste reservoir has sufficient capacity for the flush.
3. Select the
Solvents tab in the right-hand panel.
4. Select the Prime tab.
5. If using an Isolera Four system, select the Channel text box and select the cartridge position
and waste reservoir to be used.
6. Select the Path text box and press Bypass .
7. Ensure that the cartridge’s inlet and outlet tubing are coupled together.
8. Select the
Volume text box and enter the total flush volume. We recommend that you flush with
at least 30 ml (Isolera Prime), 60 ml (Isolera One and Isolera Four), or 100 ml (Isolera LS).
A Flow Cell Outlet Tube
B Flow Cell
C Flow Cell Inlet Tube
D Opened Retaining Latch
C
D
B
A