Biotage Isolera Four, Руководство пользователя

Страница: 15 / 116

411829-L, Description and Specifications April 2012 Page 1-9
1
1.2.4 Chemistry Mode
In the Chemistry mode, the software is divided into four (4) tabs that can be accessed in the right-
hand panel (see Figure 1-7). Here is a short description of what you can do at each tab:
• Method: Create, open, and edit a method, and assign it to a cartridge mounted on the
system, select the rack position(s) to be used, and start or queue up* the purification.
• Status: View information on a cartridge mounted on the system, monitor and edit the
purification in progress, enable injection of a liquid sample, and start or queue up* a
purification after a finished equilibration step. It is also possible to enable or disable the
Collect All mode, manually instruct the system to switch to a new collection vessel, start and
stop collecting through the waste channel, start and end an isocratic segment, change the
processing order of queued purifications*, and manually end (or abort) and remove a
purification.
• Results: Search and view the results of all purifications stored in the system’s database, on
a USB memory device (if connected), or in a share folder on your network (if connected).
Result reports can be printed to a network printer with postscript support or a local USB
printer and saved as PDF files on a USB memory device or in a share folder on your network.
It is also possible to create a new method with the same purification parameters as the ones
used in a previous purification. If desired, the system can help you to optimize the gradient
to isolate one of the peaks and reduce the amount of solvent used.
• Solvents: Assign solvents to the solvent inlets, set the solvent and waste reservoirs’
volumes (if monitoring of solvent and waste are enabled), prime the solvent inlets and, when
using an Isolera LS system, control the sample loading pump. It is also possible to flush a
cartridge with air to empty it of remaining solvents
†
and purge a cartridge to release any
remaining pressure before unloading it.
* Purifications can be queued up when using an Isolera Four system.
†
The Air Flush feature is not available when using an Isolera Prime system.
Figure 1-7. Chemistry Mode (Isolera LS and
Isolera Four EXP with Spektra License Shown)
Status and Task Field
The system can be idle, paused ( ), or processing ( ). When the system is processing, the
following tasks can be in progress:
• UV ON: The system turns on the detector lamp(s).
• Eq. Flush: Before an equilibration is started, the system runs an equilibration flush to empty
the solvent inlets of solvents used in the previous purification and fills them with new solvents.
Task field