© Biotage 2018
Biotage
®
Flash 400 Quick Guide | Page 1
Biotage
®
Flash 400 Quick Guide
Control Panel
Item
Description
PG-01
Pump pressure gauge. Monitors the solvent pump outlet
pressure.
PG-02
Cartridge compression pressure gauge. Monitors the
pressure inside the radial compression module.
MV-01
Solvent select valve. Selects which of the two solvent
inlets (A or B) that will be used.
MV-06
Fraction select valve. Selects which of the two outlets
(1 or 2) the elution stream will exit through.
MV-07
Cartridge compression valve.
Opens the line between the radial compression
chamber and the radial compression fluid reservoir,
which compresses the cartridge if the fluid reservoir is
pressurized. PG-02 and PG-03 readings will equalize.
Closes the valve.
MV-08
Solvent pump valve. Turns the solvent pump on or off
(O or I) by opening or closing the line between the air
supply and the pump.
MV-09
Reservoir pressure valve.
Pressurizes the radial compression fluid reservoir by
connecting it to the nitrogen supply.
Used when checking that the pressure in the radial
compression fluid reservoir can be maintained.
Vents the radial compression fluid reservoir by
opening it to the atmosphere. The exhaust port is
located on the side connection panel.
MV-11
Pump flow rate control. Regulates the compressed
air pressure to the solvent pump. Turn the knob
counterclockwise to increase the solvent flow rate and
clockwise to decrease it.
Safety Positions
Before introducing compressed air or nitrogen into the system,
ensure that the following valves are in their safety position.
Valve
Safety Position
MV-11
Clockwise until stopped
MV-08
O (closed)
MV-09
(vent)
MV-07
(open/compress)
Radial Compression Fluid Reservoir
Item
Function
PG-03
Reservoir pressure gauge. Monitors the radial
compression fluid reservoir pressure.
Side Connection Panel
Item
Description
Solvent inlets A and B.
Fraction outlets 1 and 2.
MV-10
Radial compression drain valve.
Closes the valve.
Drains the fluid from the radial compression
chamber. Used before removing the cartridge.
MV-05
Vacuum break valve, bottom.
Closes the valve.
Breaks any vacuum that develops when the cartridge
is in use. Used when preparing to remove a cartridge
or when testing if the cartridge seal adapter’s O-ring
has sealed properly.
Compressed air inlet for the solvent pump.
Nitrogen inlet. Used to pressurize the radial compression
fluid reservoir and the radial compression module.
Exhaust port for when venting the radial compression
fluid reservoir using the MV-09 valve.
Safety relief port for the radial compression module. The
module is rated at 170 psig (11.7 bar) maximum pressure.
Top Head Assembly
Item
Description
MV-02
Three-way injection valve. Selects the solvent or sample
inlet. During cartridge removal, compressed gas may be
blown through this valve and through the cartridge to
remove solvent.
MV-03
Cartridge compression vent valve. Open the valve to
purge air from the radial compression chamber when
compressing the cartridge.
MV-04
Vacuum break valve, top. Open the valve to break any
vacuum that develops when the cartridge is in use. Used
when preparing to remove a cartridge or testing if the
cartridge seal adapter’s O-ring has sealed properly.
Figure 1. The cartridge compression vent valve (MV-03) and the top
vacuum break valve (MV-04) closed (left image) and opened (right image).
