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Bioruptor NGS Скачать руководство пользователя страница 7

PAGE 7

DIAGENODE

BIORUPTOR

®

NGS

USER MANUAL

Diagenode Inc. North America

/ Phone: +1 862 209-4680 // Fax: +1 862 209-4681 // Mail: orders.na

@

diagenode.com

Getting to Know Your Bioruptor

®

NGS System

Bioruptor

®

Components Overview

Power Cable (EU)

Power Cable (US)

Control Unit Cable

Control Unit

Water bath

Motorized lid

Soundproof box

Tube holder

Bioruptor

®

Water Cooler

To learn more, please visit www.diagenode.com

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Содержание NGS

Страница 1: ...U S E R M A N U A L Version 1 5 Bioruptor NGS Cat No UCD 600 Bioruptor Sonication System...

Страница 2: ...expenses to return the repaired or replacement product This warranty covers only manufacturing defects and does not cover any damage caused by misuse lack of compliance to recommendations stated in th...

Страница 3: ...oruptor Water Cooler 7 Connector kit for Water Cooler 8 Water bath 8 Motorized lid 9 Metallic Soundproof Box 9 Tube holders 9 Equipment Installation 10 Installation overview 10 Installing the Biorupto...

Страница 4: ...Maintenance The ultrasound waves are created from a series of magnets that are attached to the water tank This system is very sensitive to the heat generated during a run Do not exceed 2 hours of tota...

Страница 5: ...tem uses ultrasound to create focused mechanical stress to shear DNA Ultrasound waves pass through the sample expanding and contracting the liquid During expansion negative pressures pull the molecule...

Страница 6: ...frequency 30 60 kHz Ultrasonic wave output power 25 210 W Transformer 220 100V dimensions Internal Control unit dimensions 350 W x 260 D x 165 H mm Sonication unit dimensions water bath 175 W x 160 D...

Страница 7: ...862 209 4681 Mail orders na diagenode com Getting to Know Your Bioruptor NGS System Bioruptor Components Overview Power Cable EU Power Cable US Control Unit Cable Control Unit Water bath Motorized li...

Страница 8: ...ion of the apparatus is required after initial set up it is imperative to keep the tank at a right angle to the ground during the transport at all times Water level and quality The level of the water...

Страница 9: ...hough this is not sufficient to avoid the temperature increase during sonication Motorized Lid The motorized lid along with the gear plate accessory keeps the sample tubes in constant rotation and ens...

Страница 10: ...under the following conditions Installation overview Indoor use Altitude up to 2 000 meters Operating external temperature 0 C to 25 C Maximum relative humidity 80 Transient overvoltage typically pres...

Страница 11: ...2 black plastic connectors on the motorized lid Both connectors can be placed into one or the other hole of the motorized lid Screw the plastic nut over the motorized lid The short red and blue pieces...

Страница 12: ...7 Unpack the Water Cooler and remove the black protection rubber which connects inlet and outlet 8 Place the solenoid valve and the male nozzle connector on the outlet and inlet as shown in the pictur...

Страница 13: ...tton on the front side of the device Temperature can be set by pushing and turning the knob on the first side of the device Note See Bioruptor Water Cooler manual for additional information Now you ar...

Страница 14: ...nctions Button A Pause restart after pausing Button B Press and hold this button during sonication to display T1 total on time per cycle and T2 total off time per cycle ESC Cancels previous selection...

Страница 15: ...earing Cat No WA 005 0500 To use the tube holder remove the lower part by turning counterclockwise Then place microtubes in the unit Attach the lower part to the upper part of the adaptor To guarantee...

Страница 16: ...Temperature 4 C Bioruptor Water Cooler Cat No BioAcc Cool Single cycle valve for Water Cooler Cat No VB 100 0001 Power setting H position High Sonication cycle total sonication time varies depending...

Страница 17: ...shown to improve reproducibility DNA quality DNA quality and quantity must be considered carefully since bad quality and quantity DNA may have several impacts on sonication and next gen sequencing dow...

Страница 18: ...he sonication water bath is a critical component of the Bioruptor sonication system 1 Water purity Contaminants such as algae and particules may alter the ultrasonic waves propagation resulting in bro...

Страница 19: ...Auto ChIP kit 1 Auto Histone ChIP seq kit 1 Auto Transcription ChIP kit 1 Features All DNA protein interaction saving time maximum reproducibility Optimized for working with histone antibodies in ChIP...

Страница 20: ...r ChIP seq and ChIP on chip Yes 1 Yes 1 Yes Yes Yes Amount of cells IP 1 000 1 million 1 10 million 1 million 100 000 1 5 2 x 10e6 Total Time of Assay 1 day 1 day 3 days 3 days 1 day Handling time 1 5...

Страница 21: ...roarray and sequencing 2 qPCR linear amplification and genome wide analysis such as microarray and sequencing 2 qPCR linear amplification and genome wide analysis such as microarray and sequencing 2 A...

Страница 22: ...genome wide analysis microarray sequencing Depending on the DNA purification method selected qPCR linear amplification and genome wide analysis microarray sequencing Depending on the DNA purification...

Страница 23: ...PAGE 23 DIAGENODE BIORUPTOR NGS USER MANUAL Diagenode Inc North America Phone 1 862 209 4680 Fax 1 862 209 4681 Mail orders na diagenode com MA BR NGS V1 5_20_04_12...

Страница 24: ...enode s a Description Cat No Bioruptor Bioruptor NGS with 0 5 0 65 ml tube holder UCD 600 TS Tube Holders 0 5 0 65 ml tube holder for Bioruptor Standard Bioruptor Plus Bioruptor NGS UCD pack 0 5 Cooli...

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