7.
Pipettors
m
10,
m
20
and
m
100:
Put a thin layer of grease on the piston (5).
Pipettors
m
200
and
m
1000:
Put a thin layer of grease around the seal (6).
Pipettor
m
5000:
Put a thin layer of grease on the seal (6) and the interior of the tip
cone cylinder (4).
Note
: Avoid excess grease. Use only the grease provided with the
pipettor.
Note
: Before reassembling check that no lint or particles are on the
surface of the piston.
Decontamination:
For complete decontamination of the lower parts place the tip ejector
collar (1), tip cone holder (2), tip cone (3) and tip cone cylinder (4) (only
the model
m
5000) into a beaker containing Biohit Proline‚ Biocontrol
and leave for at least 30 minutes. Wipe the piston with Biohit Proline‚
Biocontrol and the lint-free cloth. Rinse the parts with distilled or sterile
water. Let the parts dry. Grease the piston and seal according to the
instructions given earlier in this chapter.
Reassembling:
1.
Pipettors
m
10,
m
20,
m
100,
m
200,
m
1000:
Carefully place the tip cone (3) on the piston and attach it by
screwing the tip cone holder (2) clockwise.
Pipettor
m
5000:
Carefully place the tip cone cylinder (4) on the piston and screw
clockwise.
Make sure that the tip cone (cylinder) is properly tightened.
Avoid over tightening.
2.
Attach the tip ejector collar (1) by screwing it clockwise.
3.
Place the new tip cone filter on its place.
4.
Press the operating button several times to ensure that the grease
has spread evenly.
5.
Check the performance of the pipettor.
Note
: It is always necessary to check the performance of the pipettor
after in-house service or maintenance.
10.3. Sterilization of the pipettor
The entire
m
LINE pipettor can be sterilized by steam autoclaving at
121
O
C, (252
O
F), 1 atm for 20 minutes. Remove the tip cone filter (if fitted,
Chapter 7.3). The single channel pipettors can be autoclaved without
special preparations.
Unscrew the
lower part of the multichannel pi-
pettors by holding the connecting
collar and turning the lower part
360° counterclockwise (Fig. 10).
Put the pipettor into the sterilization
bag and place it into the autoclave.
After autoclaving the pipettor must
11
360°
Fig. 10
Connecting
collar
