8 EN
Without CO
2
the BG-Sentinel catches
Aedes aegypti, Ae
-
des albopictus and Aedes polynesiensis which are vectors
of Dengue, Chikungunya, Filariasis and Yellow fever. It also
catches some Culex species such as
Culex quinquefasciatus
and
Culex pipiens
; however, the addition of CO
2
enables you
to catch a broader range of blood seeking species as well
as much higher numbers of the previous mentioned species.
Various sources of CO
2
can be used. The optional CO
2
nozzle
can be attached to a gas cylinder, any well insulated dry ice
container or another CO
2
source that fits your preference and
that is placed away from the trap.
Fermentation of yeast:
Yeast-produced carbon dioxide can be produced by mixing
17.5 g of dry yeast, 250 g of sugar and 2½ L of tap water or
35 g of dry yeast, 500 g of sugar and 2½ L of tap water in
a 5 L bottle. Capturing the foam to prevent the foam from
entering the traps can be accomplished by a set up with two
bottles. See detailed information for this protocol in a pub-
lication at http://www.ncbi.nlm.nih.gov/pubmed/20973963.
CO
2
gas cylinders:
In order to supply the trap with the carbon dioxide (CO
2
) at-
tractant, a commercially available CO
2
gas cylinder with two
or more kilograms can also be used. These can be acquired
or rented at drink retailers or bottle depots for a deposit. A
door-to-door delivery service may also be possible. We re-
commend a 10 kg gas cylinder which is also used in restau-
rants for draft beer and soda.
Attaching the pressure reducing regulator onto the CO
2
gas
cylinder:
The BG-Sentinel CO
2
trap includes a specialized pressure
reducing regulator that is preadjusted to a constant out-
put pressure of 0.7 bars. The pressure remains consistent
throughout the entire usage. Screw the pressure reducing
regulator onto the CO
2
gas cylinder by rotating the screw on
the end of the regulator onto the opening on the CO
2
cylin-
der and make sure it is tight by using a wrench or pliers [F21].
Administering the carbon dioxide
Adjusting the CO
2
consumption with the CO
2
Y-tube
containing the respective sinter plugs:
Biogents‘ sinter technology is an elementary component in
the Biogents’ catching system which optimizes the release
of CO
2
and allows the adaptability to the local and seasonal
conditions. Depending on the existing mosquito situation,
you can choose between two different release rates: 200
grams/day and 500 grams/day. This allows the adaptation of
the carbon dioxide emission to the local and seasonal mos-
quito situation.
When monitoring mosquito populations, the 500 grams/day
release rate is recommended.
The Biogents BG-Sentinel CO
2
set includes a CO
2
tube with
three endings. The two short ends at the Y-shaped connec-
tion each contain a sinter plug and can be attached to the
pressure reducing regulator. The different sinter plugs are
each marked with a colored band and label.
Attach either the red or blue end of the CO
2
Y-tube to the
pressure reducing regulator. On the regulator with a screw on
connection, unscrew the nut on the outlet, thread the end of
the CO
2
tube through the nut, insert the end of the CO
2
tube
onto the outlet and re-screw the nut back onto the outlet
[F22].
F21
F22
