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Bioer GeneQ Thermal Cycler Скачать руководство пользователя страница 21

Gene Q Thermal Cycler

Chapter 4 Operation Guide

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15

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(Please refer to the following figure: relation between block temperature and reagent
temperature.)

“Tube mode”

has considered the phenomenon that the cooling & heating rate is a little

slower than that of block. There is an overshoot after reaching the setting temperature, which
can allow reagent to reach the setting temperature faster. During the same setting time as
“Block”, the actual time for reagent to keep the setting temperature is prolonged obviously.
(Please refer to the following figure: relation between block temperature and reagent
temperature.)

3.5

“Hotlid” stand for the state of hotlid “Open” or “Close”.

3.6

“Key Sound” and “Run End Sound” stand for the sound of pressing key and reminding sound

after the procedure runs over respectively.

Block

Reagent

Block

Reagent

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Содержание GeneQ Thermal Cycler

Страница 1: ...art of this manual shall be photocopied reproduced or translated into other languages without written permission from the Bioer Co Patent No ZL 98 2 44367 6 Standard No YZB 0189 2008 Document Number B...

Страница 2: ...cler s power line is a safety device that should be matched with a grounded socket Never let the third ground pin floating If the 3 pin plug cannot be inserted it is recommended to ask an electrician...

Страница 3: ...hould be protected against any kind of heat sources like sunlight ovens or central heating equipment If the Thermal Cycler is set aside for a long time it is recommended to disconnect the power line t...

Страница 4: ...hole and the tube inside it In case it is smeared the surface of the Thermal Cycler may be scrubbed with a piece of dehydrated soft wet cloth 7 WARRANTY AND SERVICE INFORMATION a Warranty The Thermal...

Страница 5: ...cation and unauthorized maintenance or alteration The Bioer Co makes no express warranties other than those which are described herein Any descriptions in sales promotion under specific conditions sha...

Страница 6: ...ption 6 2 Keyboard Diagram 7 3 Key Functions 7 4 Inspection before Power on 8 5 Power on Procedures 8 CHAPTER 4 OPERATION GUIDE 9 1 How to Run a PCR Program 9 2 How to edit a PCR program 10 3 How to S...

Страница 7: ...n and adjustment of DNA sequence of protein structure detection of gene extension construction of synthetic genes construction of cloning and expression carrier detection of polymorphism of a gene s i...

Страница 8: ...ree water baths cyclically and thus finishes the three processes of denaturation annealing and extension This device has high temperature control accuracy high heating cooling rate and excellent tempe...

Страница 9: ...g thermal cyclers have gradually dominated the market 5 Features of the Gene Q Thermal Cycler The Gene Q Thermal Cycler is a thermoelectric cooling thermal cycler It features a Reliable and stable ope...

Страница 10: ...20 240V 50 60Hz 200W 100 120V 50 60Hz 200W Note Before power on please check if the above operating conditions are satisfied Pay special attention to the power line s reliable grounding 2 Transportati...

Страница 11: ...situ time 99 hour 59 min Programs storage 100 5 Software Functions File editing and saving File accessing modifying and deleting Automatic file link Heating cooling ramp setting Automatic temperature...

Страница 12: ...Description Heated lid Block Block LCD display Keyboard RS232 interface Power switch Power connecter Fuse Socket Note 1 The fuse specifications are as follows 250V 2 5A or 125V 5A 5 20mm The fuse shou...

Страница 13: ...e left or right when pressing during file parameter setting Up Down moving the cursor one position to the up or down when pressing during file parameter setting Enter press it to accept the present pa...

Страница 14: ...nding of the power line is reliable 5 Power on Procedures Turn on the power i e turn the power switch to position 1 the Thermal Cycler will beep twice and the LCD will display self test That means the...

Страница 15: ...evolve the knob clockwise till it sound dang dang Revolve the knob one more turn At the beginning of revolving the knob clockwise or counter clockwise you can hear dang dang sound press the release ke...

Страница 16: ...o input sample volume according to practical condition Under Tube mode the overshooting temperature and keeping time will be dirrerent in the case of different sample volume Press Back to go back the...

Страница 17: ...terface Press Accept to confirm the password and then the system will enter file running interface of sample volume input window After press Run in the file list interface and input the password If th...

Страница 18: ...ess Seg to enter segment edit interface In every segment you can set setting temperature Temp duration Time Ramping rate Ramp temperature increment of every cycler Temp and time increment of every cyc...

Страница 19: ...ccording to practical condition Press Back to go back file save interface Press Run to enter file running interface Enther the file save interface if the user press Run without pressing Save the syste...

Страница 20: ...nal one 3 1 Date and Time stand for default time after power on 3 2 Default File and Default User stand for name and user of default running procedure after power on 3 3 Module Select stands for modul...

Страница 21: ...emperature which can allow reagent to reach the setting temperature faster During the same setting time as Block the actual time for reagent to keep the setting temperature is prolonged obviously Plea...

Страница 22: ...Upgrading Software 1 Choose and click Setup exe in the CD start up guide procedure and then press Next to continue installation refer to the picture below 2 Please confirm License Agreement of the so...

Страница 23: ...xt to enter into the installation interface 4 Please select target installation folder refer to the following picture and then press Next to enter into the installation interface The guide procedure w...

Страница 24: ...fy and press Next to edit installation subassembly press Finish to finish this step refer to the following picture Note It is necessary to select Installation file If you delete before installation fi...

Страница 25: ...ade exe under the folder of upgrading software installation the screen will show you the upgrading procedure interface refer to Figure 1 Press Expand to enter into the expansion interface refer to Fig...

Страница 26: ...Open the control panel of computer to click Device Manager and then double click COM1 as showed in Figure 3 2 Choose Advanced in Communications Port COM1 Properties Revise the COM Port Number in Adva...

Страница 27: ...elow ambient one Cooling module failure Contact distributor or manufacturer Fan failed or stopped 4 Heating and cooling terminated Bad sensor Contact distributor or manufacturer Malfunction of all coo...

Страница 28: ...ributor with Heatsink temperature is too high Heat sink temp higher than 70 the sensor broken or short circuit or the fan is not working 3 Error Message when running Please contact the distributor wit...

Страница 29: ...1_SEN LID J2 2_SEN LID J2 3_HEATER LID J2 4_HEATER CPU PCB4 BY Q5041041000000_CPU J13 1_HV J13 2_HV J13 3_VIN5 J13 4_HOTLID J13 5_FAN J13 6_PWM J13 7_HEAT J13 8_COOL J3_CPU_JTAG_IDC20 J10_KEY IN J5_LC...

Страница 30: ...TAG_IDC20 J10_KEY IN J5_LCD_24P J9_CPLD_JTAG_IDC10 J16 1_SEN HOTLID J16 2_SEN HOTLID J15 1_SEN FAN J15 2_SEN FAN J15 3_SEN BLOCK J15 4_SEN BLOCK VB1_SUB D9_RS232C J17 1_H J17 2_H L U5 1 2 3 4 5 6 LIN...

Страница 31: ...00 99 01 12 01 31 year month date 89 Time Time HH MM SS 00 00 00 23 59 59 hour minute second 910 Default File The default file name Max length is 11 characters 110 Default User The default user name...

Страница 32: ...Gene Q Thermal Cycler Appendix 2 Parameter Value Meaning 26 Picture 2 Picture1 block reagent block reagent...

Страница 33: ...NOTES...

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