BIO RAD Trans-Blot Turbo, Инструкция по эксплуатации

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2. Follow the procedure described in Figures 8-10 above for assembling the transfer sandwiches in
transfer cassettes, followed by an addtional step: once assembled, remove excess transfer buffer by
inverting the cassette base with the assembled stack carefully held in place into a waste container.
Once the excess buffer is removed, place the cassette lid on the base, and proceed with the
transfer step (3.4).
3.4 Transfer Using the Turbo Protocol
The Turbo button immediately accesses the MIXED MW program. It is designed for efficient transfer of
a wide variety of proteins over a broad range of molecular weights. The same parameters can also be
accessed in the Bio-Rad preprogrammed protocols under LIST > BIO-RAD > MIXED MW.
1. Turn on the Trans-Blot Turbo system using the switch located on the right side of the instrument.
After an initial Boot screen, the system will proceed to the Home screen.
2. On the Home screen press the Navigation button that corresponds with Turbo (Figure 12).
3. After selecting Turbo, there will be a selection screen for the number, size, and type of gel to be
transferred. Use the corresponding selection button to choose the option for the combination of
gels in the run. The option varies the current for the run (1.3 A for a single mini format gel, 2.5 A for a
single midi or 2 mini format gels). Mini PROTEAN® TGX™ gels can be transferred using the “1 Mini
Gel” protocol or the “1 Mini PROTEAN TGX” protocol, which transfers a single Mini PROTEAN TGX
gel in 3 min.
4. Press the Navigation button that corresponds to A:RUN for the cassette in the upper bay or B:RUN
for the cassette in the lower bay (Figure 13). A beep will sound to signal the start of the transfer
for the chosen cassette. If you are running both cassettes, press the button to begin one transfer
(either A:RUN or B:RUN), and then press the other button at any time during the run to immediately
start the second cassette. Both cassettes must use the same protocol.
5. The protocol will run automatically. The screen will display the conditions of the transfer and the
progress of the run.
Protocol name
Transfer conditions
during run (real time)
Star indicates an active run
Program settings
Gel format
Fig. 13. Transfer Turbo Run screen.
Fig. 12. Home Screen of the Trans-Blot Turbo system.
Selection
buttons
Navigation
buttons
Trans-Blot® Turbo™ Blotting System 11