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Catalog
Number

Product Description

Total Protein Detection Kits
170-6512

Biotin-Blot Protein Detection Kit

170-6517

Enhanced Colloidal Gold Total Protein Detection Kit

Blotting Standards
161-0372

Precision Prestained Standards, 10–250 kD, 500 µl

161-0380

Precision Streptactin-HRP conjugate

161-0381

Precision Streptactin-AP conjugate

161-0305

Prestained SDS-PAGE Standards, Low range

161-0309

Prestained SDS-PAGE Standards, High range

161-0307

Biotinylated SDS-PAGE Standards Kit, Low range, HRP

161-0308

Biotinylated SDS-PAGE Standards Kit, Low range, AP

161-0312

Biotinylated SDS-PAGE Standards Kit, High range, HRP

161-0313

Biotinylated SDS-PAGE Standards Kit, High range, AP

161-0306

Biotinylated SDS-PAGE Standards, Low range, 250 µl

161-0311

Biotinylated SDS-PAGE Standards, High range, 250 µl

Premixed Electrophoresis and Blotting Buffers
161-0732

10X Tris/Glycine/SDS, 1 L

161-0755

10X Tris/Glycine/SDS, 6 x 1 L

161-0734

10X Tris/Glycine, 1 L

161-0757

10X Tris/Glycine, 6 x 1 L

161-0733

10X Tris/Boric Acid/EDTA, 1 L

161-0756

10X Tris/Boric Acid/EDTA, 6 x 1 L

161-0741

10X TBE Extended Range, 1 L

161-0758

10X TBE Extended Range, 6 x 1 L

Section 3
Safety Instructions

Read the entire manual before beginning electrophoretic transfers.

Electrophoretic transfer of proteins and nucleic acids is dependent on many factors.

Observe the following guidelines to avoid mishaps that may result in serious damage to the
instrument or injury to the operator.

1. Do not reverse polarity on this instrument. This will result in corrosion and rusting of

the stainless steel cathode. If this should occur, the stainless steel should be cleaned with
a mild abrasive cleaner to remove the rust.

2. Do not exceed 25 V with this instrument. This could damage the electrodes.

3. Do not adjust the pH of transfer buffers unless specifically indicated. Follow instruc-

tions carefully. Adjustment of pH of transfer buffers, when not indicated, will result in
increased buffer conductivity. This is manifested by a higher than expected initial current
output as shown by the power supply's current meter. Monitor buffer resistance with the
Model 200/2.0 power supply prior to each run to insure proper buffer conductivity.

5

Содержание Trans-Blot SD

Страница 1: ...Trans Blot SD Semi Dry Electrophoretic Transfer Cell Instruction Manual Catalog Number 170 3940 For Technical Service Call Your Local Bio Rad Office or in the U S Call 1 800 4BIORAD 1 800 424 6723...

Страница 2: ...in the instrument during this warranty period Bio Rad Laboratories will repair or replace the defective parts free The following defects however are specifically excluded 1 Defects caused by improper...

Страница 3: ...10 Section 5 Buffer Formulation 10 Section 6 Examples of Specific Protocols 11 6 1 SDS Protein Blotting 11 6 2 DNA Blotting For acrylamide gels with DNA 250 bp to 1 kb 12 6 3 DNA RNA Blotting For agar...

Страница 4: ...er which served as the ion reservoir and replaced the buffer tank The plate electrodes separated only by the filter paper stack provided high field strength V cm across the gel and very efficient rapi...

Страница 5: ...EAN xi Gels 30 170 3969 Sheets 19 x 19 5 cm pre cut to fit PROTEAN xL Gels 30 Immuno Blot PVDF Membrane 162 0174 Sheets 7 x 8 4 cm 10 mini blot size 162 0175 Sheets 10 x 15 cm 10 162 0176 Sheets 20 x...

Страница 6: ...190 Sheets 9 x 12 cm 15 162 0191 Sheets 10 x 15 cm 15 162 0192 Sheets 15 x 15 cm 15 162 0193 Sheets 15 x 20 cm 15 162 0194 Sheets 20 x 20 cm 15 162 0195 Sheets 20 x 25 cm 15 162 0196 Roll 30 cm x 3 3...

Страница 7: ...assays on blotted membranes Catalog Number Product Description Immun Blot Assay Kits 170 6460 Immun Blot Assay Kit Goat Anti Rabbit AP 170 6461 Immun Blot Assay Kit Goat Anti Mouse AP 170 6462 Immun B...

Страница 8: ...Boric Acid EDTA 1 L 161 0756 10X Tris Boric Acid EDTA 6 x 1 L 161 0741 10X TBE Extended Range 1 L 161 0758 10X TBE Extended Range 6 x 1 L Section 3 Safety Instructions Read the entire manual before be...

Страница 9: ...rformed by Bio Rad or an authorized agent IEC 1010 1 is an internationally accepted electical safety standard for laboratory instruments Section 4 Trans Blot SD Assembly To determine the optimum condi...

Страница 10: ...bubbles can block transfer of molecules To avoid membrane contamination always use forceps or wear gloves when handling membranes 4 Cut filter paper to the dimensions of the gel Two pieces of extra th...

Страница 11: ...omplete if any portion of the gel is outside the blotting media Roll out all air bubbles 5 Place the other sheet of pre soaked filter paper on top of the gel carefully removing air bubbles from betwee...

Страница 12: ...0 2 0 power supply is capable of a 200 watt output This means that unless a current limit is set uncontrolled conductivity changes may result in full power being delivered to the Trans Blot SD cell In...

Страница 13: ...Plug the unit into the power supply red wire to red outlet and black wire to black outlet Caution Do not reverse polarity This will damage the stainless steel cathode 9 Turn on the power supply Transf...

Страница 14: ...10 mM NaCHO3 3 mM Na2 CO3 20 methanol pH 9 9 Dissolve 0 84 g NaHCO3 and 0 318 g Na2 CO3 anhydrous in dd H2 O add 200 ml of methanol adjust volume to 1 liter with dd H2 O DO NOT ADD ACID OR BASE TO AD...

Страница 15: ...e from the gel and rinse the membrane briefly in 0 5x TBE buffer 6 Fix the DNA to the membrane by placing the membrane on several pieces of blot paper saturated with 0 4 N NaOH for 10 minutes 7 Rinse...

Страница 16: ...may be performed without significant loss of primary bound protein Even small proteins appear to bind stably Zeta Probe membrane cannot be dye stained as destaining is impossible Instead the Biotin Bl...

Страница 17: ...conductivity improp erly prepared buffer will result in excessive power delivered to the cell 8 2 Poor Binding to Nitrocellulose Membrane 1 Proteins separated by SDS PAGE require 20 methanol in the t...

Страница 18: ...elrod V D Gorbulev V G Belzhelarskya S N and Vartikyan R M Anal Biochem 100 129 1979 11 Peudelhuber T L Ball D J Davis A H and Garrad W J Nuc Acids Res 10 1311 1982 12 Danner D B Anal Biochem 125 139...

Страница 19: ...852 2789 1257 India Ph 91 11 461 0103 Fx 91 11 461 0765 Israel Ph 03 951 4127 Fx 03 951 4129 Italy Ph 39 02 216091 Fx 39 02 21609 399 Japan Ph 03 5811 6270 Fx 03 5811 6272 Korea Ph 82 2 3473 4460 Fx...

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