BIO RAD Trans-Blot SD Скачать руководство пользователя страница 1 | Manualshive

Страница: 1 / 19

background image

Trans-Blot

®

SD

Semi-Dry

Electrophoretic

Transfer Cell

Instruction

Manual

Catalog Number

170-3940

For Technical Service Call Your Local Bio-Rad Office or in the U.S. Call 1-800-4BIORAD (1-800-424-6723)

1
2
3
...
»

Содержание Trans-Blot SD

Страница 1: ...Trans Blot SD Semi Dry Electrophoretic Transfer Cell Instruction Manual Catalog Number 170 3940 For Technical Service Call Your Local Bio Rad Office or in the U S Call 1 800 4BIORAD 1 800 424 6723...

Страница 2: ...in the instrument during this warranty period Bio Rad Laboratories will repair or replace the defective parts free The following defects however are specifically excluded 1 Defects caused by improper...

Страница 3: ...10 Section 5 Buffer Formulation 10 Section 6 Examples of Specific Protocols 11 6 1 SDS Protein Blotting 11 6 2 DNA Blotting For acrylamide gels with DNA 250 bp to 1 kb 12 6 3 DNA RNA Blotting For agar...

Страница 4: ...er which served as the ion reservoir and replaced the buffer tank The plate electrodes separated only by the filter paper stack provided high field strength V cm across the gel and very efficient rapi...

Страница 5: ...EAN xi Gels 30 170 3969 Sheets 19 x 19 5 cm pre cut to fit PROTEAN xL Gels 30 Immuno Blot PVDF Membrane 162 0174 Sheets 7 x 8 4 cm 10 mini blot size 162 0175 Sheets 10 x 15 cm 10 162 0176 Sheets 20 x...

Страница 6: ...190 Sheets 9 x 12 cm 15 162 0191 Sheets 10 x 15 cm 15 162 0192 Sheets 15 x 15 cm 15 162 0193 Sheets 15 x 20 cm 15 162 0194 Sheets 20 x 20 cm 15 162 0195 Sheets 20 x 25 cm 15 162 0196 Roll 30 cm x 3 3...

Страница 7: ...assays on blotted membranes Catalog Number Product Description Immun Blot Assay Kits 170 6460 Immun Blot Assay Kit Goat Anti Rabbit AP 170 6461 Immun Blot Assay Kit Goat Anti Mouse AP 170 6462 Immun B...

Страница 8: ...Boric Acid EDTA 1 L 161 0756 10X Tris Boric Acid EDTA 6 x 1 L 161 0741 10X TBE Extended Range 1 L 161 0758 10X TBE Extended Range 6 x 1 L Section 3 Safety Instructions Read the entire manual before be...

Страница 9: ...rformed by Bio Rad or an authorized agent IEC 1010 1 is an internationally accepted electical safety standard for laboratory instruments Section 4 Trans Blot SD Assembly To determine the optimum condi...

Страница 10: ...bubbles can block transfer of molecules To avoid membrane contamination always use forceps or wear gloves when handling membranes 4 Cut filter paper to the dimensions of the gel Two pieces of extra th...

Страница 11: ...omplete if any portion of the gel is outside the blotting media Roll out all air bubbles 5 Place the other sheet of pre soaked filter paper on top of the gel carefully removing air bubbles from betwee...

Страница 12: ...0 2 0 power supply is capable of a 200 watt output This means that unless a current limit is set uncontrolled conductivity changes may result in full power being delivered to the Trans Blot SD cell In...

Страница 13: ...Plug the unit into the power supply red wire to red outlet and black wire to black outlet Caution Do not reverse polarity This will damage the stainless steel cathode 9 Turn on the power supply Transf...

Страница 14: ...10 mM NaCHO3 3 mM Na2 CO3 20 methanol pH 9 9 Dissolve 0 84 g NaHCO3 and 0 318 g Na2 CO3 anhydrous in dd H2 O add 200 ml of methanol adjust volume to 1 liter with dd H2 O DO NOT ADD ACID OR BASE TO AD...

Страница 15: ...e from the gel and rinse the membrane briefly in 0 5x TBE buffer 6 Fix the DNA to the membrane by placing the membrane on several pieces of blot paper saturated with 0 4 N NaOH for 10 minutes 7 Rinse...

Страница 16: ...may be performed without significant loss of primary bound protein Even small proteins appear to bind stably Zeta Probe membrane cannot be dye stained as destaining is impossible Instead the Biotin Bl...

Страница 17: ...conductivity improp erly prepared buffer will result in excessive power delivered to the cell 8 2 Poor Binding to Nitrocellulose Membrane 1 Proteins separated by SDS PAGE require 20 methanol in the t...

Страница 18: ...elrod V D Gorbulev V G Belzhelarskya S N and Vartikyan R M Anal Biochem 100 129 1979 11 Peudelhuber T L Ball D J Davis A H and Garrad W J Nuc Acids Res 10 1311 1982 12 Danner D B Anal Biochem 125 139...

Страница 19: ...852 2789 1257 India Ph 91 11 461 0103 Fx 91 11 461 0765 Israel Ph 03 951 4127 Fx 03 951 4129 Italy Ph 39 02 216091 Fx 39 02 21609 399 Japan Ph 03 5811 6270 Fx 03 5811 6272 Korea Ph 82 2 3473 4460 Fx...

Отзывы:

Нет отзывов

Похожие инструкции для Trans-Blot SD

Бренд: Labnet Страницы: 2

Бренд: Ecco Страницы: 2

Бренд: Landoll Страницы: 94

Бренд: Rain-Flo Irrigation Страницы: 20

Attest Auto-reader 390

Бренд: 3M Страницы: 2

Бренд: 3M Страницы: 2

Бренд: 3M Страницы: 2

PELTOR WS LiteCom Plus

Бренд: 3M Страницы: 7

Steri-Vac GS Series

Бренд: 3M Страницы: 52

Бренд: Datex-Ohmeda Страницы: 80

Бренд: MacDon Страницы: 2

Бренд: V-TAC Страницы: 6

Бренд: Federal Signal Corporation Страницы: 4

Бренд: FASTERHOLT Страницы: 96

Бренд: Tascam Страницы: 8

Бренд: Kärcher Страницы: 88

ULTRATHERM 908i

Бренд: gbo Страницы: 32

Бренд: Dantel Страницы: 6

Бренды по названию

0 1 2 3 4 5 6 7 8 9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Популярные бренды

Загрузить еще бренды