Section 3
Connection to
Low-Pressure
Chromatography Systems
Bio-Scale Mini cartridges are ideal for use with
Bio-Rad's BioLogic™ LP chromatography system,
Econo™ gradient pump, the patented* Model
EP-1 Econo pump, and all low-pressure
chromatography instruments. Bio-Scale Mini cartridges
can be conveniently connected directly to the system
using the luer fittings on the cartridge.
1. Install 1.6 mm inner diameter (ID) tubing in the
pumphead. Adjust the platen pressure screw
(on pumphead) using a screwdriver or coin.
Turn the screw counterclockwise as far as it will
go, then turn clockwise three full turns.
Assemble with fittings and lock rings as shown
in Figure 1.
* US patent 5,135,658
7
Reagent
Group
Description
Stability
Denaturants
Guanidine-HCl
Do not use in lysis and
wash buffers.
Urea
Up to 2 M with no drop
in binding capacity. At
4 M there may be some
loss of binding capacity.
At 8 M, binding capacity
and target protein purity
will be reduced.
Other additives
CaCl
2
£
5 mM when used with
MES, MOPS, or PIPES
buffers. Do not use with
phosphate buffers.
MgCl
2
£
5 mM. Do not use with
fluoride containing
buffers. Use NaN
3
as an
alternate triggering ion.
For a complete list of chemical compatibility, refer to the Profinity eXact
Purification System instruction manual.
.
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