L5-TB-047Q
November 2007
SW 41 Ti Rotor
Used In Beckman Coulter
Class H, R, and S
Preparative Ultracentrifuges
© 2007 Beckman Coulter, Inc.
Printed in the U.S.A.
Страница 1: ...L5 TB 047Q November 2007 SW 41 Ti Rotor Used In Beckman Coulter Class H R and S Preparative Ultracentrifuges 2007 Beckman Coulter Inc Printed in the U S A...
Страница 2: ...ng appropriate safety precautions Biosafe containment should be used when Risk Group II materials as identified in the World Health Organization Laboratory Biosafety Manual are handled materials of a...
Страница 3: ...24 k factors at maximum speed 5 to 20 sucrose gradient 5 C When particle density 1 3 g mL 335 When particle density 1 5 g mL 307 When particle density 1 7 g mL 295 Conditions requiring speed reduction...
Страница 4: ...the crossbar of the rotor hanger mechanism Gaskets made of Buna N rubber between each bucket and bucket cap maintain atmospheric pressure inside the buckets during centrifugation NOTE On some swinging...
Страница 5: ...facturers components or accessories in the SW 41 Ti rotor may void the rotor warranty and should be prohibited by your laboratory safety officer Only the components and accessories listed in this publ...
Страница 6: ...s in the bucket caps are clean and lightly but evenly lubricated with Spinkote lubricant Put bucket caps on the buckets and use a screwdriver to screw the caps into the buckets until there is metal to...
Страница 7: ...d with other rotors 2 Refer to the instrument instruction manual for ultracentrifuge operation 3 For additional operating information see the following RUN TIMES page 10 for using k factors to adjust...
Страница 8: ...Temperature Limits Plastic tubes have been centrifuge tested for use at temperatures between 2 and 25 C For centrifugation at other temperatures pretest tubes under anticipated run conditions If plas...
Страница 9: ...ccessory Max Speed RCF k Factor Dimensions and Volume Description Part Number Description Part Number 14 x 89 mm 13 2 mL Ultra Clear 344059 pkg 50 none 41 000 rpm 288 000 x g 124 14 x 89 mm 13 2 mL th...
Страница 10: ...If necessary float mineral oil or some other low density immiscible liquid on top of the tube contents to fill the tube to its maximum volume Do not use an oil overlay in Ultra Clear tubes All opposin...
Страница 11: ...actor for Estimating Run Times from Previously Established Run Conditions publication DS 719 RUN SPEEDS The centrifugal force at a given radius in a rotor is a function of speed Comparisons of forces...
Страница 12: ...gal Field g k Factor At rmax 153 1 mm At rav 110 2 mm At rmin 67 4 mm 41 000 40 000 36 000 288 000 274 000 222 000 207 000 197 000 160 000 127 000 121 000 97 800 124 130 160 35 000 30 000 25 000 210 0...
Страница 13: ...ent and must be limited so that CsCl precipitation is avoided Speed and density combinations that intersect on or below the curves in Figure 3 ensure that CsCl will not precipitate during centrifugati...
Страница 14: ...ntrifugation The dashed lines are representa tions of equation 6 and are shown here to illustrate the inability of that equation to predict CsCl precipitation 1 10 1 00 10 0 20 30 40 41 1 20 1 30 1 40...
Страница 15: ...ds from Figure 2 results in gradients presented here 1 10 1 00 67 4 88 8 3 4 filled 1 2 filled 1 4 filled 110 2 131 6 153 1 1 20 1 30 1 40 1 50 1 60 1 70 1 80 1 90 Density g mL SW 41 Ti ROTOR 20 C 4 C...
Страница 16: ...ntrifuged at 41 000 rpm see Figure 2 The segment of the 41 000 rpm curve Figure 3 from the quarter filled line to the tube bottom represents this gradient The same solution in a half filled tube may b...
Страница 17: ...ure 3 sketch in a horizontal line corresponding to each particle s buoyant density 2 Select the curve at the required temperature 4 C and tube volume full that gives the best particle separation 3 Not...
Страница 18: ...bes for the chemical resistances of rotor and accessory materials Your Beckman Coulter representa tive provides contact with the Field Rotor Inspection Program and the rotor repair center CLEANING Was...
Страница 19: ...nic materials follow appropriate decontamina tion procedures as outlined by your laboratory safety officer Refer to Appendix A in Rotors and Tubes to select solutions that will not damage the rotor an...
Страница 20: ...safety officer regarding proper methods to use Refer to publication IN 192 included with each box of tubes for tube sterilization and disinfection procedures Quick Seal Ultra Clear and thinwall open...
Страница 21: ...ersonnel it is the customer s responsibility to ensure that all parts are free from pathogens and or radioactivity Steriliza tion and decontamination must be done before returning the parts Smaller it...
Страница 22: ...s and gaskets 333790 Bucket caps set of 6 331763 Bucket gasket 331309 Overspeed disk 41 000 rpm 330335 Rotor stand 332400 Bucket holder rack 331313 OTHER Tubes and accessories see Table 1 Quick Seal C...
Страница 23: ...e for maintenance of good laboratory practices Repair and Replacement Policies 1 If a Beckman Coulter rotor is determined by Beckman Coulter to be defective Beckman Coulter will repair or replace it s...
Страница 24: ...6 Taiwan Republic of China Telephone 886 2 2378 3456 Fax 886 2 2377 0408 TURKEY Beckman Coulter Ltd E 5 Yanyol Faith Cad 81410 Soganlik Kartal Istanbul Turkey Telephone 90 216 309 1900 Fax 90 216 309...
