BD FACSJAZZ, Руководство пользователя

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lasted updated by MW on 11/10/2017
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Sort Set up
1. Turn on the chiller if you would like to use it.
2. In the sort layout window, under sort devices choose the device you would like to sort into.
3. Place the appropriate tube holder in the sorting arm with the appropriate dummy tubes.
4. Click SORT READY in the sort layout window.
5. Click TEST STREAM .
6. Adjust the stream deflections till the streams hit the center of the tubes.
7. Click TEST STREAM to turn off your test streams.
8. In the inspector choose the path to save your data. (C:\Influx\your name\)
9. Change the Prefix to the name of your sample. Make sure you are choosing Prefix and not FileName.
10. Adjust the events to display to 10,000.
11. Vortex and load your on to sample port and press SAMPLE .
12. Press ACQUIRE in the acquisition bar.
13. Adjust the event rate to less than 10,000 events/second.
14. Press SAMPLE to stop running your sample.
19. Press STOP in the acquisition bar to stop acquiring.
15. Draw your gates.
16. Add your sorting gates to the sort layout.
17. Make sure you change the sort mode to 1.0 Drop Pure .
18. Load your collection tubes.
19. Press SAMPLE to start running your sample.
20. Press ACQUIRE in the acquisition bar.
21. Press START to sort your sample.
22. Choose the number of events you would like to record.
23. Press RECORD .
While your sample is running:
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Safe a PDF of your worksheet by selecting the PDF icon and saving it to C:\Influx\your name.
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Monitor your stream and gap.
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Monitor your collection tubes so they don’t over fill.
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Monitor the sort streams.
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Keep your event rate less than 10,000 events per second.
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Do NOT increase your offset above 5.0 PSI, however idly not above 2.0 PSI.
24. Press STOP in the sort window to stop the sort.
25. Press STOP RECORDING in the acquisition bar to stop recording.
26. Safe a PDF of your sort counts by selecting SORT REPORT PREVIEW button on the sort layout
window. Safe the file to C:\Influx\your name.
27. Press BACKFLUSH , to remove your cells from the sample line.
28. Repeat acquisition and sorting steps for as many samples as necessary.