lasted updated by MW on 11/10/2017
1
BD FACSJazz Users Guide
Satchmo
Stanford Shared FACS Facility
PPE must be worn at all times in our BSL-2 environment
*Lab coat, gloves, and closed toed shoes are mandatory; respirator and goggles are available*
An Instrument Configuration is located on the website in the
Instrument Overview area
*The configuration lists the lasers and filters for each machine*
All samples must be in POLYPROPYLENE round bottom tubes.
What samples do I need to bring?
•
All users
ALWAYS
need to bring single stained compensation controls
•
If you have a 4 color panel you should bring: 5 compensation controls
o
One tube stained with each individual antibody
o
One unstained tube
o
No PI or viability dye in the single color controls
Don’t have enough cells for compensation?
User can purchase compensation beads from many different suppliers
•
eBioscience UltraComp Beads 01-2222-42
(for panels including UV and Violet dyes)
•
eBioscience OneComp Beads 01-1111-42
(for panels that do NOT include any UV or Violet dyes)
•
BD CompBeads 552845 ‘Anti-Rat/Hamster’
•
BD CompBeads 552843 ‘Anti-Mouse’
•
ThermoFisher Amine Reactive Compensation Beads A10628
(for LIVE/DEAD Fixable stains)
