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20 BD FACSCanto II Flow Cytometer Reference Manual
Excitation Optics
The excitation optics consist of lasers, fiber optic cables, beam-shaping prisms,
and an achromatic focusing lens, as shown in Figure 1-3.
The BD FACSCanto II instrument uses low-powered air-cooled and solid state
lasers that do not have special power and cooling requirements.
Fiber optic cables direct the laser light onto beam-shaping prisms, which in turn
transmit the laser light to a focusing lens. The lens directs the laser light onto the
sample stream within the flow cell (Figure 1-3). See also Figure A-3 on page 109.
Figure 1-3 Optical pathway (figure shows violet laser available with 4-2-2 configuration only)
When the flow cell access door opens, an interlock shutters the laser light and
blocks its pathway for safety reasons.
Table 1-1 4-2 configuration fluorochromes
Laser
Wavelength
(nm)
Commonly Used Fluorochromes
Coherent® Sapphire™
Solid State 488 (blue) FITC, PE, PE-Cy7,
PerCP or PerCP-Cy5.5
JDS Uniphase™ HeNe
Air Cooled 633 (red) APC, APC-Cy7
1
2
3
4
interlock focusing lens
prisms flow cell
1 3
2
4