Azure Imaging Systems
User Manual
for the Azure 200, Azure 280, Azure 300,
Azure 400, Azure 500, Azure 600
NIR
RGB
CHEMI
WHITE
LIGHT
BLUE
UV
Rev 20190919
Страница 1: ...Azure Imaging Systems User Manual for the Azure 200 Azure 280 Azure 300 Azure 400 Azure 500 Azure 600 NIR RGB CHEMI WHITE LIGHT BLUE LIGHT UV Rev 20190919 ...
Страница 2: ...any UV protection devices such as the safety switch on the light cabinet apparatus are working properly If not discontinue use until the device s are properly repaired Use only UV lamps in the transilluminator and ensure they are the correct size and voltage Electrical Safety Precautions Be sure to take proper precautions when handling any electrical equipment NEVER work on any live circuit fixtur...
Страница 3: ...losed within the system enclosure and there are no viewing ports windows or openings to facilitate viewing of or exposure to radiation fields from direct impact reflection or leakage It is possible to override the safety switch which allows the user to turn on the light sources with the door open which may be useful for band excision in UV Maintenance Azure Biosystems 500 and 600 laser systems do ...
Страница 4: ...orized agent Use of spare parts supplied by anyone other than Azure Biosystems Damage caused by accident or misuse Damage caused by disaster Corrosion caused by improper solvents or samples Voltage Setting Information The Azure Imaging System has a power supply that automatically chooses the correct voltage for your country or region CE Conformity The following Azure Imaging Systems models 200 280...
Страница 5: ...B Input Devices 8 3 Capture Software Overview 9 3 1 The home page contains the following imaging options 9 3 2 Key definitions that apply to all imaging types 10 3 3 Imaging Workflow for Chemiluminescent Applications 11 3 4 Imaging Workflow for Fluorescent Applications 12 3 5 Imaging Workflow for Nucleic Acid Gel 12 3 6 Imaging Workflow for Protein Gel 13 3 7 Imaging Workflow for Custom Image 14 4...
Страница 6: ...ter Wheel The 7 position filter wheel is automated It includes the standard Orange filter for most UV fluorescent applications and additional Red Green Blue and or IR filters for multichannel systems Integrated Computer The touch screen PC is completely integrated for system control and image acquisition Black Tray Stored in the front door the Black Tray also called Blot Tray is required for imagi...
Страница 7: ... x 45 cm Weight 22 kg 49 lbs 1 2 Contacting Technical Support For questions about installation setup or general use of the system please contact support azurebiosystems com or call 925 307 7127 9am 4pm Pacific time Azure 600 Azure 500 Azure 400 Azure 300 Azure 280 Azure 200 Upgradable to 600 Upgradable to 600 Upgradable to 400 500 and 600 Upgradable to 300 400 500 and 600 NIR RGB CHEMI WHITE LIGHT...
Страница 8: ...st light leaks 2 2 Connecting to Power The power entry module is located in the lower right on the back panel of the system Connect the power cord to a secure power outlet It is important to connect the system to a well grounded power source Azure Biosystems recommends that you employ a surge protecting power strip to protect against unexpected power surges that may cause damage to the electrical ...
Страница 9: ...tware application If the software has been closed double click on the desktop icon Once the system is done initializing the software displays the gallery tab and is ready to use Please contact Azure technical support at support azurebiosystems com for questions about upgrading the software 2 5 Other USB Input Devices You may attach regulatory approved Windows OS supported USB keyboard USB mouse or...
Страница 10: ... 3 Nucleic Acid Gel image nucleic acid blots See Section 3 5 for additional details 4 Protein Gels image protein blots See Section 3 6 for additional details 5 Custom select your preferred features from any of the other imaging modalities See Section 3 7 for additional details Other options include 6 Protocol selection use to save and load custom imaging protocols See Section 4 1 for details 7 Aut...
Страница 11: ...e software to capture image for you using the full dynamic range of the camera There are four possible modes Underexpose software will optimize a short exposure time to the brightest signal on the blot Weaker signals may not be visualized in an underexposed image This is the fastest method Overexpose software will calculate an exposure time that saturates the brightest bands in the blot to potenti...
Страница 12: ...f your dye does not appear on the list contact Azure Technical Support 7 Next select your imaging protocol Select Autoscan if you are not sure of your exposure time For single channel image chemi only The system defaults to 20 images with autoexpose Note this number is customizable in the settings tab It s like watching your blot develop incrementally before your eyes Select the best image in the ...
Страница 13: ...ee Section 3 2 for details 10 If you are interested in repositioning your sample before you take the image use Live Mode See Section 3 2 for details 11 Select to capture the image 12 The images will be displayed in the Gallery The Gallery can be reached by clicking the tile icon See Section 4 1 for instructions on using the Gallery 13 Select to go back one screen 3 5 Imaging Workflow for Nucleic A...
Страница 14: ...kflow for Protein Gel Use this for protein gel applications e g polyacrylamide gel electrophoresis PAGE 1 Remove the black blot tray and place sample in center of tray Make sure to place the blot in the center of the tray 2 Close the Azure Imaging System door 3 Select Nucleic Acid Gel from the home screen 4 Select dyes in between one and four channels red green blue and grayscale 5 Pick the name o...
Страница 15: ...lect a focus value around the default setting Note For Azure 200 the focus does not need to be adjusted for the UV table or black tray 6 Select Exposure type See Section 3 2 for a detailed explanation of the exposure types 7 Add Dye add another set of options from numbers 3 6 above e g Excitation emission channel aperture focus and exposure times Can add up to four for the four channels red green ...
Страница 16: ...en you return to the gallery 2 From the Print dropdown menu you can Print print to a local printer connected to the system or to a network printer Print Report print the image you re viewing as well as data about the image These include Exposure type Bin Level Gain Filter Calibration Software version System SN 3 Create a Multiplex image by merging between two and four channels 4 Create Signal Chan...
Страница 17: ...these dyes choose any number of Chemi Fluorescent Protein Gel and Nucleic Acid Select File Saving Settings Can choose to enable autosave and or saving a publication image Simulation Mode allows you to run the software even if not connected to an Azure Imaging System Single Imaging Mode allows you to select the number of images in cumulative The default is 10 Auto Exposure Rapid capture will enable...
Страница 18: ...s edit or create Custom Protocols Select Edit button to the right of existing protocols Or select light source name filter whether you want cumulative multiple or single images the default focus aperture and exposure and the live step and preview step settings ...
Страница 19: ...ed The Azure Biosystems logo and Azure Biosystems are trademarks of the Company All other trademarks service marks and trade names appearing in this brochure are the property of their respective owners www azurebiosystems com info azurebiosystems com ...
