Image the Digital PCR 20K Chips
1.
Set the destination for the imaging data:
a.
From the Main Menu of the QuantStudio
™
3D
Instrument, touch Destination.
b.
In the Destination screen, touch the desired destination
for the imaging data.
2.
If you choose to use a USB drive as a destination for your
imaging data, insert the drive into the USB port on the front
of the instrument.
3.
Open the chip tray and load the Digital PCR 20K Chip face-
up into the bay. Confirm that the Digital PCR 20K Chip is
correctly aligned within the chip tray, then close it.
X0300036
1
2
1
Orient the chip ID and fill port toward the front of the
instrument.
2
Confirm that the chip window is clean and correctly aligned
to the chip.
4.
In the instrument touchscreen, touch Start Run, then wait for
the QuantStudio
™
3D Instrument to image the chip.
IMPORTANT!
Do not open the chip tray or remove the USB
drive (if present) while the instrument displays the
countdown screen. Doing so will invalidate the image data
and require you to repeat the run.
5.
When the touchscreen displays the Analyzing Chip screen,
open the chip tray and remove the Digital PCR 20K Chip.
Store or discard it as desired.
You can read another Digital PCR 20K Chip without waiting
for the analysis to complete. To begin the next run, load the
chip and touch Start Run as described in step 3.
6.
After reviewing the results of the run, touch Done to close
the results screen.
If the analysis of the imaging data produced a (red) for
either probe (FAM
™
or VIC
®
dye), visually inspect the chip
for problems and read the chip again.
After a Digital PCR 20K Chip has been read:
• You run the imaged Digital PCR 20K Chip again for up to
1 hour after thermal cycling.
• If you read multiple Digital PCR 20K Chips in rapid
succession, touch the scroll buttons ( / ) to review the
results of the previously imaged chips.
• The QuantStudio
™
3D Instrument retains a copy of the
analyzed data for the imaged Digital PCR 20K Chip that you
can access from the Run History screen.
IMPORTANT!
Confirm that the QuantStudio
™
3D Instrument is
unloaded before you power off and move it. If the instrument is
moved while loaded, the Digital PCR 20K Chip inside can rotate
within the chip tray and obstruct the chip drawer, preventing it
from closing completely.
8
QuantStudio
™
3D Digital PCR System Quick Reference