Applied Biosystems GeneAmp PCR System 9700, Руководство пользователя

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5-6 Creating and Editing Methods
Creating Methods
About the Default
Method
The GeneAmp PCR System 9700 comes with a default PCR thermal profile called a
method. The create screen displays this default method. For information on displaying
the create screen, see “Displaying the Create Methods Screen” on page 5-7.
You can run the default method shown above, or use it as a template to create a
customized method.
Basic Parameters
To create a method, you need to define the following four basic parameters:
♦
Temperature Control Parameters
♦
Pre-PCR Holds
♦
PCR Parameters
♦
Post-PCR Holds
For detailed information about these parameters refer to the table below:
Pre-PCR segment PCR segment Post-PCR segment
Number of
temperature
control
parameters
in the
pre-PCR
segment
Number of
temperature control
parameters in the
PCR segment
Number of cycles the
PCR segment
will run
Number of
temperature
control parameters
in the post-PCR
segment
1 Hld 3 Tmp 25 Cycles 2 Holds
94.0 94.0
5:00 0:30
55.0
0:30
72.0
0:30
72.0
7:00
4.0
F1 F3 F4 F5 F2
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Entering Temperature Control Parameters 5-8
Defining Pre-PCR Holds 5-9
Defining PCR Parameters 5-9
Defining Post-PCR Holds 5-10