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Specification

Sensitivity

1 nmol/l FAM at 30 µl
Sample volume

1 nmol/l FAM at 10 µl
Sample volume

Measuring time

approx. 6 s for a simple measurement, 6 colors

Measurement range

± 130 000 (±17 bit)

Dynamic range

10 log stages

Measuring principle

Fiber-optic shuttle system
with 8 optical fibers

Fiber-optic shuttle system
with 16 optical fibers

Light source

Four-color high-performance LEDs (blue, green, white, red)

Detector

Highly-sensitive PMT (Photo Multiplier Tube)
Optimum signal/noise characteristics due to efficient noise
suppression

Filter modules



12 color-, FRET- and protein modules



6 positions in the device

Safety features



Sample chamber is monitored by sensors



Overtemperature protection in the heated lid



Interlock switch (front flap)

Noise emissions

< 45 dB

Regulations/certificates

See “Standards and directives” on page 42

10.1.2

Power module

Designation/type

qTOWER

auto power module

Dimensions
(width x height x depth)

290 x 186 x 335 mm

Mass

approx. 12 kg

Protection type

IP 20

Safety class

Voltage range*

100 / 115 / 230 V

Max. power

950 W

Frequency

50/60 Hz

Fuse

2x 10 AT / 250 V

Output ports

3 (Power, Data 1, Data 2)

Safety features



Overload detection



Ground fault



Overheating detection



Sheathed connectors and sockets

Regulations/certificates

See “Standards and directives” on page 42

qPCR
application

Fluorescence
spectrometer

Other
characteristics

General
data

Electrical
characteristics

Other
characteristics

Содержание qTOWER3

Страница 1: ...Operating manual qTOWER3 auto qTOWER3 84 auto Real time PCR thermal cycler ...

Страница 2: ... 49 551 50 68 666 E mail service ls analytik jena com Analytik Jena GmbH Konrad Zuse Str 1 07745 Jena Germany Phone 49 3641 77 9460 Fax 49 3641 77 946 E mail support ls analytik jena com http www analytik jena com 10 3107 082 23 A 01 2021 Analytik Jena GmbH Copyright 2021 Analytik Jena GmbH Manufacturer Technical Service Application Support General Information Documentation number Edition Technica...

Страница 3: ...ction 14 3 1 Layout 14 3 1 1 qTOWER auto 16 3 1 2 qTOWER3 auto power module 18 3 2 Function 19 3 2 1 Fluorescence spectrometer 19 3 2 2 PCR thermal cycler 21 3 2 3 Lid heating 21 3 2 4 Plastic 21 3 3 Type plate 22 4 Installation and commissioning 23 4 1 Power supply 23 4 2 Unpacking and inspection 24 4 3 Installing and connecting the device 25 5 Operation 27 5 1 Switching on the qTOWER auto 27 5 2...

Страница 4: ... with extended drawer 17 Fig 4 qTOWER auto rear view 17 Fig 5 Power module front view 18 Fig 6 Power module rear view 18 Fig 7 Schematic representation fluorescence spectrometer 19 Fig 8 Schematic representation light beam course through filter color module 20 Fig 9 qTOWER3 auto type plate 22 Fig 10 qTOWER3 auto power module type plate 22 Fig 11 Connecting the qTOWER3 auto 25 Fig 12 Connections at...

Страница 5: ... and servicing of the device as well as potential causes and remedies of any faults Instructions in chronological sequence are numbered grouped into blocks of instruc tions and the expected result is indicated Safety instructions are indicated by pictographs and signal words The type source and consequences of the danger are stated together with notes on preventing the danger The meaning of the pi...

Страница 6: ...FRET modules here up to six of which can be installed simultaneously in the device In addition to the optical components for the detection of real time signals the amplifi cation of the target sequences plays a central role With Peltier technology and the use of high quality block materials the qTOWER3 auto boasts a superb thermal homogene ity maximum speed and accuracy The qTOWER auto is fully co...

Страница 7: ...tended use described in this operating manual result in limitations of warranty and liability during a damage event Warranty and liability claims are ex cluded for personal injury and property damage due to one or several of the following causes Improper use of the qTOWER auto Improper commissioning operation and maintenance of the device Modifications of the equipment without prior consultation w...

Страница 8: ...ssing safety symbols can cause incorrect actions leading to personal in jury or material damage Do not remove safety symbols Replace damaged safety sym bols immediately The following safety symbols are attached to the front of the power supply unit or to the rear of the device Safety symbol Meaning Risk of electric shock Do not touch General warning sign Warning about hot surface Disconnect the pl...

Страница 9: ...ll not cause any environmental danger or health risk within this period of time if the device is used as intended 2 2 2 Symbols The following symbols are used on the device and or its packaging Symbol Meaning CE marking Manufacturer and address Year of manufacture Order number Temperature limit Serial number Note Observe the operating manual Caution In combination with the symbol above Attention O...

Страница 10: ...ith the appropriate care and kept clean in accordance with laboratory standards to prevent contamination of the device Observance of these laboratory rules reduces the risk of being contaminated with potentially infec tious material and the risk of cross contamination of the samples Wear protective gloves and take other safety measures if there is a chance that your skin may come into contact with...

Страница 11: ...k can cause liquids to evaporate explosively Only use plates and tubes that are suitable for high temperatures up to 110 C Do not touch hot sample tubes or plates and do not open them or boiling liquid may escape 2 5 2 Safety instructions protection against explosion and fire Do not operate the qTOWER auto with flammable explosive or volatile substances Do not operate the qTOWER auto in a potentia...

Страница 12: ...nments with extreme humidity 95 or in locations in which condensation may occur 2 5 4 Handling of samples auxiliary and operating materials The operator is responsible for the selection of substances used in the process as well as for their safe handling This is particularly important for radioactive pathogenic infec tious poisonous corrosive or otherwise hazardous substances For details contact t...

Страница 13: ...fety equipment ac cident and danger alarms as well as first aid and rescue equipment as well as its use The operator is responsible for the respective training of the operating personnel All equipment for first aid first aid kit eyewash bottles stretcher etc as well as equipment for firefighting fire extinguishers must be within reach and easy to ac cess All equipment has to be in a sound conditio...

Страница 14: ... microtiter plate can be easily posi tioned on the drawer without any problems The patented high performance optics comprising a fiber optic shuttle system and unique light source guarantee a superb homogeneous excitation and illumination of all individual samples The light source comprises 4 high performance LEDs with a broad spectrum reaching deep into the red In combination with specially optim...

Страница 15: ...Layout and function 15 Fig 1 Device components 1 qTOWER auto 2 qTOWER 84 auto 3 qTOWER3 auto power module 1 2 3 ...

Страница 16: ...art with fluorescence photometer 2 Status LED indicates whether the device is switched on or off 3 Drawer 4 Block cycler unit The drawer serves to hold the sample carrier micro titer plate Once a sample carrier has been positioned on the drawer and moved into the device this is positioned above the thermal block and the front flap closes and locks automatically ATTENTION Never attempt to pull the ...

Страница 17: ...ed in Fig 4 are attached to the rear side of the qTOWER auto beside the fans Fig 4 qTOWER auto rear view 1 USB port for PC connection 2 Type plate 3 Fan grating 4 Electrical connections Data 1 connection lid heating communication lines Data 2 connection connection of thermal block sensors Power line connection power 1 2 3 4 ...

Страница 18: ...or Data 1 Data 2 and Power are also located here Fig 5 Power module front view 1 Status LED indicates whether the device is switched on or off 2 Power connection 3 Data 1 connection lid heating communication lines 4 Data 2 connection for connecting the sensors of the thermal block A mains connection socket the fuse compartment and the power switch are located at the back of the qTOWER auto in addi...

Страница 19: ...arm up period Fig 7 The light is passed through optic fibers to collimator lenses bundled and then trans ferred to the excitation filter of the color modules fitted to a rotating filter wheel The light is deflected via a beam divider and passed through additional optical fibers to a lens array in a shuttle system scanning the sample block in columns Fig 7 Multi plexer The fluorescent dyes are spec...

Страница 20: ...modules 6 color modules for the most frequently used fluorescent dyes from the blue to the red excitation range 5 filter modules specially optimized for FRET applications and 1 protein module for melting curve analysis A retrofitting or replace ment of the installed modules may be carried out by the Analytik Jena Service team at any time ATTENTION If you intend to extend the application spectrum o...

Страница 21: ...protects the Peltier elements and prolongs the lifetime of the device 3 2 3 Lid heating The qTOWER auto comes equipped with an automated heated lid This can be adjusted to temperatures of up to 110 C and prevents condensation forming in the area of the reaction tubes above the block surface level Furthermore the heated lid guarantees regardless of the consumables used a reliable contact between th...

Страница 22: ...e rear of the respective device unit and includes the fol lowing information Manufacturer address incl logo Device designation trade name Information for device identification such as serial number etc Technical data incl electrical connection data Safety symbols such as CE marking etc Disposal instructions Do not dispose of as domestic waste ...

Страница 23: ...ice at a minimum distance of 15 cm between the rear of the device and the wall or other objects A minimum distance of 10 cm should be maintained on both sides Ensure that the ventilation slits are clear Insufficient ventilation may cause the device to overheat Take into consideration the space required for the control PC also monitor and printer where applicable 4 1 Power supply WARNING Observe th...

Страница 24: ... to Analytik Jena 1 Unpack the device by removing all protective film fastenings such as adhesive tape etc 2 Check the delivery for completeness and ensure that it matches your order Standard scope of delivery Components Amount qTOWER auto 1 Power module 1 Power cable 1 USB connection cable 1 Data cable for data 1 and data 2 connec tions 2 Power cable 1 qTOWER auto operating manual EN 1 Software C...

Страница 25: ... power module on a sturdy even surface in a secure dry place Leave the device switched off to allow it to acclimatize adequately 2 hours 1 Make sure the power switch at the back of the power module is OFF before connect ing the power cable to a grounded power outlet 100 V to 230 V Take note of the voltage rating on the type plate beside the power cable on the back of the power module Note Make sur...

Страница 26: ...s at rear 3 Connect the power cable and the two data connection cables both at the qTOWER auto and the power module Fig 11 The connection cables circular con nectors are fitted with a push pull locking mechanism for this purpose i e the con nector automatically locks into place before removing it first slide back the outer sleeve ATTENTION Remove the transport lock during commissioning 4 Switch on...

Страница 27: ...r example to prepare a real time PCR project first the software normally detects the qTOWER auto automatically 4 If not device identification with the qTOWER auto will need to be manually initi ated To do this click on EXTRAS in the menu bar and activate the item DEVICE IDENTIFI CATION 5 A line of communication is created between the control software and the device There is a status LED on the fro...

Страница 28: ... the wells these must be sealed with a transpar ent optical adhesive foil sealing foil Note The optical transparency of the foils affects the fluorescence signal directly Therefore only use clear adhesive foil as offered for real time PCR 2 Prepare a real time PCR project with complete details about the PCR run fluores cence measurements and the sample layout of the PCR plate see qPCRsoft auto man...

Страница 29: ...OWER auto see page 30 X Check electrical components and cables test the grounding conductor qualified electricians only X All maintenance and repair work beyond this scope may only be performed by Analytik Jena Service personnel or authorized and trained persons Any unauthorized interven tion limits warranty entitlements If the device exhibits any faults or defects please con tact the Analytik Jen...

Страница 30: ...leaning once they are completely dry ATTENTION The use of concentrated alcohol organic solvents or abrasives for cleaning can damage the device housing If the devices become contaminated during daily use cleaning with a damp cloth is suffi cient Only wipe the device housing with a soft clean cloth which may be wetted with a com mercially available neutral cleaning agent if necessary 6 2 Checking t...

Страница 31: ...terial great care must be taken because the qTOWER auto cannot be decontaminated as a complete device Only the housing and the drawer of the device may be disinfected Immediately remove visible contamination by suitable means Do not allow solvents to enter the device Recommended disinfectant for the housing Incidin Pro Supplier Ecolab Deutschland GmbH Pay attention to the efficacy spectrum of the ...

Страница 32: ...n the fuse compartment Note When a power cable is plugged into the power connector opening the fuse compartment is not possible Fig 13 Fuse compartment at the back of the qTOWER auto power module 4 Pull the fuse holder out of the compartment and check the fuse If the fuse has blown or the fuse element shows signs of damage replace the fuse with an identical fuse type 2 x 10 AT 250 V Note Only use ...

Страница 33: ...s the status at the time of printing more current information may be available please contact the manufac turer service for more information There are some error correction measures that can be performed by the customer These are listed in the following chapter 7 2 Information on errors or malfunctions Error malfunction Possible cause s Suggested solution Power supply interrupted Check all connect...

Страница 34: ...ple carrier jammed Position error If the sample carrier is already in the device contact service Drawer does not move Mechanical or electrical failure Contact service Drawer makes strange noises when moving Contact service ...

Страница 35: ...at major tempera ture fluctuations are avoided and the formation of condensate is thus prevented 8 1 1 Inserting the transport lock The transport locks must be inserted before the qTOWER3 auto is packaged Further information can be found in separate packaging instructions 8 1 2 Moving the qTOWER auto in the laboratory CAUTION Risk of injury if the device falls Disconnect the mains connection and t...

Страница 36: ...n Disinfecting the device page 31 Download the decontamination declaration as an editable PDF document in German and English from the Internet see https www analytik jena com fileadmin content service customer Declara tion_of_decontamination_en_01 pdf Complete the form and attach the signed decontamination declaration to the outside of the shipment Only use the original packaging for the shipment ...

Страница 37: ...low in dust and free from aggressive vapors If the device is not installed immediately after delivery or not required for prolonged pe riods it should be stored in its original packaging A suitable desiccant should be added to the packaging to prevent damage from moisture Further information on the air conditioned storage requirements for the qTOWER auto can be found in the section Environmental c...

Страница 38: ... occur dur ing measurements sample materials in accordance with the statutory and local regula tions At the end of their service life the qTOWER3 auto and the qTOWER 3 auto power mod ule with their electronic components must be disposed of as electronic waste in accord ance with valid regulations ...

Страница 39: ...Silver gold plated Aluminum special alloy Sample capacity 96 384 Sample volume 10 100 μl 2 30 μl 5 20 μl recommended Heating max 8 C s max 4 C s Cooling max 5 5 C s max 2 C s Temperature setting range 4 C 99 C Temperature uniformity 55 C 0 15 C after 15 s Temperature control accu racy 0 1 C Heating rate adjustment min 0 1 C s Temperature uniformity after 15 seconds 0 15 C at 55 C 0 25 C at 72 C 0 ...

Страница 40: ...evice Safety features Sample chamber is monitored by sensors Overtemperature protection in the heated lid Interlock switch front flap Noise emissions 45 dB Regulations certificates See Standards and directives on page 42 10 1 2 Power module Designation type qTOWER3 auto power module Dimensions width x height x depth 290 x 186 x 335 mm Mass approx 12 kg Protection type IP 20 Safety class I Voltage ...

Страница 41: ...ly be changed by service if necessary 10 2 Environmental conditions Operation Transport storage Temperature range 15 C to 35 C 10 C to 55 C Max humidity 70 10 to 30 Use desiccant Max permissible height 2000 m Air pressure 0 7 to 1 06 bar Work environment Only suitable for operation in rooms ...

Страница 42: ...he qTOWER auto was built and tested in compliance with the standards given The re quirements of the EU directives 2014 35 EU and 2014 30 EU were also met Custom ers receive devices that are in a sound condition with regard to technical safety To maintain this condition and to ensure safe operation the operator must observe the safety and operating instructions contained in this operating manual Th...

Страница 43: ...and country specific regulations have to be complied with Besides the safety instructions in this operating manual and the local safety regulations that apply to the operation of the qTOWER auto the generally applicable regulations regarding accident prevention occupational health and safety and environmental pro tection must be observed and complied with ...

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