Analytik Jena 95-0030-01 Скачать руководство пользователя

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81-0169-01 Rev W

UVP Hybridizer, UVP HybriLinker and UVP
Multidizer Hybridization Ovens

Operating Instructions

UVP Hybridizer UVP Multidizer UVP HybriLinker

IMPORTANT:

To familiarize yourself with the correct operation procedures, read these instructions

before operating your UVP Hybridizer, UVP Multidizer or UVP HybriLinker system.

_________________________________________________________________________

Analytik Jena US

2066 W. 11th Street, Upland, CA 91786
Tel: (909) 946-3197
Fax: (909) 946-3597

Web Site:

www.us.analytik.jena.com

Ultra-Violet Products Ltd.

Unit 1, Trinity Hall Farm Estate

Nuffield Road, Cambridge CB4

1TG UK

Tel: +44(0)1223-420022 Fax:

+44(0)1223-420561

Содержание 95-0030-01

Страница 1: ...ead these instructions before operating your UVP Hybridizer UVP Multidizer or UVP HybriLinker system _________________________________________________________________________ Analytik Jena US 2066 W 1...

Страница 2: ...Contents Table of Contents 2 Introduction 3 Hybridization Oven Specifications 4 Setup Instructions 5 Operating Instructions 7 Care and Cleaning 12 Replacement Parts 13 Accessories 14 Maintenance Repai...

Страница 3: ...ironment is calibrated at 68 0 C At this temperature the accuracy is 0 5 C An internal circulating fan assists in uniformly distributing heating throughout the chamber Two different hybridizer bottle...

Страница 4: ...d a crosslinker in one space saving unit Each section has separate power supplies for independent operation Part Numbers Volts Hz 95 0031 01 115V 60Hz 95 0031 02 230V 50Hz 95 0031 03 100V 50Hz Specifi...

Страница 5: ...wenty 20 35 x 150 mm bottles or ten 10 35 x 300 mm bottles In addition combinations of both bottle sizes may be used at the same time To insert bottle holder carousel Fig 1 slide the two wheels onto t...

Страница 6: ...ns Page 6 81 0169 01 Rev W WheelS Rod Thumb Screw Fig 1 Inserting Bottle Holder Carousel Fig 2 Adjusting for Bottle Lengths Fig 3 Inserting Bottles Fig 4 Offset Skewed Bottle Positioning Fig 2 Adjusti...

Страница 7: ...cked in place To remove the tray reverse the process To install the Orbital Motion Tray optional on all units open the chamber door remove the carousel connect the power cable to the orbital tray and...

Страница 8: ...ge the setpoint from normal mode proceed as follows 7 Momentarily press then release either the UP or the DOWN button The LED display will flash to indicate that the displayed value is the current set...

Страница 9: ...devices Use of this equipment other than as intended may create a safety hazard and or malfunction 1 Place the unit on a level working surface Be sure there is adequate room in front of the door to op...

Страница 10: ...ng to your desired UV exposure time on the touchpad The energy exposure settings should now be displayed as flashing on the LCD NOTE Your energy exposure settings displayed must be multiplied by 100 I...

Страница 11: ...e Crosslinker by holding down STOP on the tactile touchpad and then turning the power to the unit ON 6 A sequence of tones will be heard from the Crosslinker and a 180 second count will display on the...

Страница 12: ...licate glass which provides superior long term reliability Each bottle includes a polypropylene cap and PTFE seal It is important to check your bottles regularly for chips stress fractures and cracks...

Страница 13: ...or Handle Compression Latch 1 22 0110 01 Power Cord 115V 1 58 0085 01 Power Cord 230V UK 1 58 0085 02 Power Cord 230V Euro 1 58 0085 03 Replacement Parts for HybriLinker Crosslinker DESCRIPTION QTY NE...

Страница 14: ...1 76 0064 03 Drip Tray for Lower Chamber 2 10 0292 02 Hood Acrylic for Upper Chamber 1 10 0317 01 Roller Assembly 2 76 0309 01 Roller Insert and Cam Assembly 1 76 0309 02 Reciprocating Shaker Tray 10...

Страница 15: ...r chamber 76 0089 01 Capacity 4 35 x 300mm 8 35 x 150mm or 8 50mm Conical Other Equipment Rocker Tray for all models 12 5W x 10D in 318 x 254 mm 98 0067 03 Removable plate is manufactured of stainless...

Страница 16: ...J Warranty AJ warrants its products to be free of defects in materials and workmanship for a period of one 1 year from date of purchase The foregoing warranty of AJ shall be of no force and effect if...

Страница 17: ...sms of Nucleic Hybridization Hybridization occurs with a process called nucleation whereby the two separate nucleic acid strands come into close proximity of each other A duplex region is formed where...

Страница 18: ...solution Unacceptable high background Use less probe Hybridize at lower salt higher temperature Wash with lower salt higher temperature Incubate with very low salt change nuclease solution Use a small...

Страница 19: ...eaction buffer 10X 50mM MgCl2 10mM 2 Mercaptoethanol 500 mM Tris HCl pH 7 5 Tagged nucleotide fluorescene 11 dUTP Template DNA in water 5ng mL Procedure 1 Pipette 10 mL of template DNA plus 10 mL of w...

Страница 20: ...hield UVP Minidizer or Hybridizer Hybridization Oven Procedure 1 Add 15 mL of prehybridization solution to each hybridization bottle containing the blot Remove bubbles between the glass and blot Cap t...

Страница 21: ...s is a good time to quickly pass your hand held radioisotope reader beta or gamma counter over your blot to get a general idea as to the exposure time you will need for the X ray film Hot blots are 20...

Страница 22: ...n Procedure 1 Mix equal volumes of detection reagents 1 and 2 2 Pipette the mixture over the surface of the membrane and leave at room temperature for 1 minute 3 Drain the sample and wrap it transfer...

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