Amersham Biosciences MegaBACE 1000, Руководство по планированию

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Chapter 3 Emission filters, chemistry, and laboratory materials
p3-6
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MegaBACE 500 and 1000 Planning Guide
3.3 Laboratory equipment
In addition to the chemistry, you need to supply the laboratory equipment
listed below.
• Microcentrifuge for 1.5–2.0-ml microcentrifuge tubes (optionally
refrigerated)
• Centrifuge rated to 3 100 g with microplate-format rotor (optionally
refrigerated)
• Micropipettors with volume ranges of 1–10 µl, 10–100 µl, 20–200 µl,
and 100–1 000 µl and tips (optional 8-channel autopipettors in 10-µl and
250-µl volumes)
• Tube racks
• Pipettes with volumes of 5 ml, 10 ml, and 25 ml
• Heat block or 96-well thermal cycler able to maintain 95 °C
• Squirt water bottle
•
−
20 °C storage for the samples and reagents
• 4 °C storage for the linear polyachrylamide (LPA) matrix and buffer and
the genotyping size standards
• Ice bucket
• Assorted beakers, flasks, and graduated cylinders
The following equipment is optional depending on your application protocol:
• Speed Vac™ concentrator with microplate-format buckets
• Balance, 1–500
±
0.1 g
• 1-liter graduated cylinder
• Pyrex ™ or equivalent glass dish measuring 20.32 cm by 20.32 cm by
5.08 cm (8 in by 8 in by 2 in) (recommended for dialysis procedure)
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