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Содержание MegaBACE 1000

Страница 1: ...service in house repair center WE BUY USED EQUIPMENT Sell your excess underutilized and idle used equipment We also offer credit for buy backs and trade ins www artisantg com WeBuyEquipment REMOTE IN...

Страница 2: ...MegaBACE 500 and 1000 Planning Guide Revision 6 December 2002 user manual 256 003 63004918 SV 399 326 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Страница 3: ...lecular Systems and F Hoffmann La Roche Ltd A license to use the PCR process for certain research and development activities accompanies the purchase of certain reagents from licensed suppliers such a...

Страница 4: ...e license arrangements from PE Applied Biosystems The above patent rights are granted solely for research and other uses that are not unlawful No other licenses are granted expressly impliedly or by e...

Страница 5: ...Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Страница 6: ...production 2 4 2 3 System electrical requirements 2 4 2 3 1 Uninterruptible power supply UPS 2 5 2 3 2 Instrument and power supply fan module North America and Japan 2 5 2 3 3 Instrument and power su...

Страница 7: ...apter 4 Planning for computer network and data storage 4 1 Basic MegaBACE system configuration 4 1 4 2 Configuration for a high throughput laboratory 4 2 4 3 Storage requirements 4 2 Chapter 5 Moving...

Страница 8: ...es for the instrument MegaBACE instrument administrator s guide provides information on how to tailor the instrument control software for your laboratory and applications MegaBACE analysis software us...

Страница 9: ...Netherlands Tel 01 576 0616 10 Tel 0165 580 410 Fax 01 576 0616 27 Fax 0165 580 401 Belgium Norway Tel 0800 73 888 Tel 2318 5800 Fax 03 272 1637 Fax 2318 6800 Canada Portugal Tel 1 800 463 5800 Tel 2...

Страница 10: ...lows you to perform detailed analysis of the data This chapter provides a summary of Preinstallation tasks section 1 1 MegaBACE system components section 1 2 Additional recommended components section...

Страница 11: ...checklist 1 2 MegaBACE system components The MegaBACE system consists of integrated hardware figure 1 1 software reagents chapter 3 and capillary arrays The basic MegaBACE system components are MegaBA...

Страница 12: ...line fluctuations See section 2 3 1 for details 1 4 Network The MegaBACE instrument and the workstation are connected using a computer network Instrument connection PCI peripheral component interconn...

Страница 13: ...Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Страница 14: ...need to plan the space accordingly See chapter 4 for workstation configurations 2 1 1 Instrument space requirements The dimensions of the MegaBACE instrument are Width 1 033 m 40 7 in Height 0 812 m...

Страница 15: ...nce for chamber door 0 914 m 36 in 1 160 m 45 7 in Laser exhaust port 1 524 mm 60 in 1 219 m 48 in width by 0 914 m 36 in depth minimum Notes 1 Allow 0 127 m 5 in clearance behind the instrument for s...

Страница 16: ...se You can order the customized table product number 94 9900 28 from the following site Amersham Biosciences Europe GmbH Munzinger Strasse 9 D 79111 Freiburg Postfach 5480 D 790 21 Freiburg Germany 49...

Страница 17: ...You can choose to vent the hot air out the back of the power supply fan module into a duct To do this requires a 0 102 m 4 in flange Contact MegaBACE System Technical Support for details The airflow...

Страница 18: ...Note A UPS will most likely require electrical connections at the wall that differ from those of the MegaBACE instrument Contact the UPS manufacturer or MegaBACE System Technical Support for details...

Страница 19: ...lease refer to the manufacturer s guidelines and follow all applicable local electrical codes during the site preparation Contact the UPS manufacturer or MegaBACE System Technical Support for more inf...

Страница 20: ...figuration A single nitrogen source for both high and low pressure supplies High pressure for injecting and removing the matrix Low pressure for rinsing the capillaries and operating the cathode and a...

Страница 21: ...ring information for the manifold assemblies Option 2 Ordering separate parts If you are using multiple instruments contact MegaBACE System Technical Support for assistance See Assistance in the prefa...

Страница 22: ...ay 0 003 m3 0 1 ft3 Table 2 5 Ordering information for nitrogen systems outside the US Item Description Suggested supplier company name telephone number Product or Code number Europe N2 Panel for Mega...

Страница 23: ...itrogen pressure To prevent pressure loss during a cycle you should check the available nitrogen pressure on a regular basis to determine when to replace the cylinders 2 4 6 Installing a nitrogen cyli...

Страница 24: ...rious applications The topics are Emission beamsplitters and filters section 3 1 Chemistry section 3 2 Laboratory equipment section 3 3 Consumables section 3 4 3 1 Emission beamsplitters and filters 3...

Страница 25: ...TAMRA A 4 585DF20 Table 3 2 Beamsplitters and filters for the DYEnamic ET primers ET primer dye ET primer base order Spectral channel Filter Beamsplitter ET R6G A 1 555DF20 A 540DRLP ET R110 C 2 520DF...

Страница 26: ...notyping filter set 2 Dye set 2 Alternatives for dye set 2 are ET ROX FAM TAMRA and HEX or ET ROX FAM TAMRA and JOE Color Spectral channel Filter Beamsplitter ET ROX Red 1 610DF20 A 540DRLP FAM Blue 2...

Страница 27: ...rimer Set 5 ATT TAG GTG ACA CTA TAG 3 20 C 2000 reactions per pack US79850 T7 ET Primer Set 5 TAA TAC GAC TCA CTA TAG GG 3 20 C 2000 reactions per pack US79855 21 M13 Forward ET Primer Set 5 TGT AAA A...

Страница 28: ...207 01 Table 3 8 Chemistry requirements for SNP genotyping Item Storage conditions Quantity Supplier Part number You can order the reagents from your local Amersham Biosciences sales office For a comp...

Страница 29: ...ks Pipettes with volumes of 5 ml 10 ml and 25 ml Heat block or 96 well thermal cycler able to maintain 95 C Squirt water bottle 20 C storage for the samples and reagents 4 C storage for the linear pol...

Страница 30: ...wrsp com Within US 1 800 932 5000 Outside US 908 465 4045 or refer to Web site 20170 217 Capillary arrays 16 capillary arrays Amersham Biosciences see the preface 63 0008 84 Capillary window cleaners...

Страница 31: ...om contact html Within US 1 800 752 8585 Outside US 408 734 8500 or refer to Web site 1055 00 X 10 plates standard 1055 01 X 10 plates sterile 1055 90 X 50 plates Note X designates a number for plates...

Страница 32: ...035 89 Be sure to verify that you order the correct type of plate for your instrument s cathode assembly The skirted plate version of the cathode assembly is convertible to the Robbins plate design If...

Страница 33: ...mables for cleanup of the SNuPe reaction Item Quantity Supplier Product code AutoSeq 96 dye terminator cleanup kit 10 pack 100 pack Amersham Biosciences see http www amershambiosciences com for site l...

Страница 34: ...t information 4 1 Basic MegaBACE system configuration The basic configuration for the MegaBACE system includes one MegaBACE instrument and one workstation figure 4 1 A PCI SCSI connects the two compon...

Страница 35: ...ation If you plan to have an additional analysis workstation that is not in the same room with the instrument control workstation make sure you have the proper network drops for the computer connectio...

Страница 36: ...nt Warning The MegaBACE instrument weighs approximately 272 kg 600 lb The instrument requires adequate physical support Never attempt to lift the instrument without using proper equipment and trained...

Страница 37: ...ninterruptible power supply UPS is recommended to reduce the instrument s vulnerability to power line fluctuations 5 3 Connecting a new computer To connect your MegaBACE instrument to a new computer f...

Страница 38: ...the Amersham Biosciences MegaBACE representative will need this information to schedule your MegaBACE system installation Organization name User manager name s Telephone Email Address Additional stor...

Страница 39: ...ptacles appropriate for your country sections 2 3 2 and 2 3 3 Provide a phone line in the lab recommended for talking to MegaBACE Technical Support Laboratory Provide a laboratory bench rated to hold...

Страница 40: ...rmal cycler Microplates 96 well Plate sealer Capillary arrays Deionized filtered water Squirt water bottle Ice bucket Wet ice Assorted beakers and flasks Uninterruptible power supply optional but reco...

Страница 41: ...d matrix LPA MegaBACE sequencing standards M13mp18 strand DNA pUC18 pUC19 STR genotyping chemistry See section 3 2 for ordering information MegaBACE long read matrix LPA and buffer MegaBACE loading so...

Страница 42: ...cols and procedures Instrument hardware components System startup and shutdown Workflow Capillary array installation and replacement Changing optical filters System software System startup Setting the...

Страница 43: ...intenance and troubleshooting System specifications Cleaning the cathode and anode Cleaning the air filter Cleaning the water tanks and bottles Check if completed Tasks and subtasks Artisan Technology...

Страница 44: ...can be located below the laboratory bench The UPS if used can be located on the floor near the laboratory bench 0 279 m 11 in 0 563 m 21 in 0 393 m 15 5 in Instrument control workstation Computer and...

Страница 45: ...Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Страница 46: ...2 C 1 weight 2 3 configurations dye set and emission 3 3 cylinders see nitrogen D data transfer and storage 4 2 dimensions computer 2 2 instrument 2 1 laboratory bench 2 2 power supply fan module 2 2...

Страница 47: ...2 5 1 nitrogen cylinder 2 10 instrument airflow requirement 2 4 clearance above 2 1 dimensions 2 1 2 2 electrical requirements 2 4 to 2 6 moving 5 1 space requirements 2 1 2 2 C 1 weight 2 3 instrumen...

Страница 48: ...trument 5 1 S SCSI connection 1 3 sequencing chemistry requirements 3 4 consumables 3 7 dyes and emission optics 3 1 laboratory equipment 3 6 site information form A 1 site requirements 2 1 to 2 10 5...

Страница 49: ...Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Страница 50: ...service in house repair center WE BUY USED EQUIPMENT Sell your excess underutilized and idle used equipment We also offer credit for buy backs and trade ins www artisantg com WeBuyEquipment REMOTE IN...

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