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Amersham Biosciences AKTAexplorer, Руководство по установке

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6

Installation test

18

ÄKTAexplorer Installation Guide 18-1139-59 Edition AC

6 Installation test

The installation test is designed to check the function of the liquid 
delivery and the UV monitoring system of ÄKTAexplorer. The 
installation test can also be used at any time to check the condition of 
the system, e.g. after a prolonged stop.

Correct gradient formation is tested by producing a linear gradient and 
a series of concentration steps of acetone. 

Correct UV monitoring is tested by monitoring the acetone 
concentration at 265, 254 and 280 nm and calculating the absorbance 
ratios 265 nm/254 nm and 265 nm/280 nm.

6.1

Preparation of ÄKTAexplorer

6.1.1

Startup of the ÄKTAexplorer separation unit

1

Switch on the separation unit using the mains switch located to the 
left on the base platform.

6.1.2

Startup of the computer and UNICORN software

1

Switch on the computer, the display and the printer according to the 
instructions in the manufacturer manuals.

2

Log into Windows

®

 by first pressing 

Ctrl-Alt-Del

, and then clicking 

OK

.

3

When the Windows desktop appears, start UNICORN by double-
clicking on the UNICORN icon.

4

Select user 

default

 and enter 

default

 as password. Click 

OK

.

ÄKTA

explorer

Mains switch

18113959AC_TK.book  Page 18  Monday, June 23, 2003  4:45 PM

Содержание AKTAexplorer

Страница 1: ...18 1139 59 Installation Guide KTAexplorer 18113959AC_TK book Page i Monday June 23 2003 4 45 PM...

Страница 2: ...18113959AC_TK book Page ii Monday June 23 2003 4 45 PM...

Страница 3: ...user may be required to take suitable measures Terms and Conditions of Sale Unless otherwise agreed in writing all goods and services are sold subject to the terms and conditions of sale of the compan...

Страница 4: ...18113959AC_TK book Page iv Monday June 23 2003 4 45 PM...

Страница 5: ...ing the computer and the mains cable 16 6 Installation test 18 6 1 Preparation of KTAexplorer 18 6 2 Priming and purging Pump P 900 19 6 3 Preparing the installation test 21 6 4 Running the installati...

Страница 6: ...Contents vi KTAexplorer Installation Guide 18 1139 59 Edition AC 18113959AC_TK book Page vi Monday June 23 2003 4 45 PM...

Страница 7: ...c are enclosed in paper boxes or in Box 900 located at the top of the instrument pile This guide describes how to install KTA explorer The guide is divided into two parts one describing the installati...

Страница 8: ...ical unit while the lamp is ON WARNING KTAexplorer must be connected to a grounded mains socket WARNING There must always be a sample loop or Superloop connected to ports 2 and 6 of the injection valv...

Страница 9: ...short circuit WARNING If heavier instrument components such as the pump are removed from the rack and the door is then opened fully the shift in the centre of gravity of the system may cause it to tip...

Страница 10: ...220 240 V 50 60 Hz electrical supply with safety grounding Cutting pliers are recommended for cutting plastic straps A waste flask is needed The installation test requires the following solutions 1000...

Страница 11: ...e first two sections of the installation record 30 Prepare KTAexplorer for the installation test 18 Run the installation test method 22 Evaluate the gradient 27 Evaluate the step response 27 Evaluate...

Страница 12: ...nvenient nearby place 2 Release and remove the red strap 1 holding the system to the pallet 3 Lift KTAexplorer onto the work area using the four strap handles 2 Two people are required to lift the sys...

Страница 13: ...t valve using the G14 capillary 6 Place the FR 904 flow restrictor 14 in the hole in the pH flow cell holder 7 Mount the pH electrode 16 according to the figure for experiments where pH is to be measu...

Страница 14: ...e column selection valves V2 and V3 Note To release the column holders press the slide clamp 18a 18b downward 2 Install the tubing holder 19 the tube holders 20 and the sample tray 21 by pushing them...

Страница 15: ...calibration of the sample pump refer to the Pump P 960 User Manual 5 4 Connecting tubings 1 Turn KTAexplorer on its swivel platform to access the rear and remove the plastic bag from the inlet tubing...

Страница 16: ...ollector optional is described in detail in the KTAexplorer Optional Configurations User Manual Refer to the section that describes your fraction collector 5 5 Installing the computer and the mains ca...

Страница 17: ...e circled figure Do not switch on 5 If using a fraction collector connect a mains cable supplied 38 between the fraction collector and a mains socket at the rear of KTAexplorer 6 Complete the two firs...

Страница 18: ...5 254 and 280 nm and calculating the absorbance ratios 265 nm 254 nm and 265 nm 280 nm 6 1 Preparation of KTAexplorer 6 1 1 Startup of the KTAexplorer separation unit 1 Switch on the separation unit u...

Страница 19: ...urge the pump P 900 as follows 1 Immerse the filters of inlet tubing A11 and B1 in the distilled water Note Never place the reservoir flask below the level of the pump inlet 2 Connect a male Luer syri...

Страница 20: ...02 MPa KTAexplorer 100 to check pump module A Run 10 ml min at 0 B distilled water Check on the pump display that the pressure reading is stable variation 0 2 MPa 3 KTAexplorer 10 to check pump modul...

Страница 21: ...ion valves 3 In the System Control module select menu Manual Flowpath Then select ColumnPosition Position1Bypass 4 Check that the correct mixer chamber is installed in the mixer 0 6 ml in KTAexplorer...

Страница 22: ...In UNICORN Main menu select File Printer Setup Select the appropriate printer from the list and select Landscape Then click OK to acknowledge the printer chosen 2 Click the Instant Run button The Inst...

Страница 23: ...c evaluation procedure that includes the autosampler peaks The name of the procedure has the extension _A90X 5 Click Next in the Method Information window 6 Click START in the Result Name window to st...

Страница 24: ...allation Guide 18 1139 59 Edition AC 8 Click the Curves tab 9 Select the following curves to be displayed UV1_265nm UV2_254nm UV3_280nm Conc Clear all other highlighted curves Click OK 18113959AC_TK b...

Страница 25: ...out consists of a chromatogram and an evaluation of the test result If the gradient test result is OK the print out says Gradient linearity accepted If the step response test result is OK the print ou...

Страница 26: ...280nm 03 SMTH Wizard Generated001 1_Conc 5 Click OK 6 Right click in chromatogram window and select Marker 7 Make sure that the UV1 curve value is displayed 8 Read the absorbance for the steps corresp...

Страница 27: ...r the results in column 3 2 Divide each value in column 3 by the base line corrected value corresponding to 100 B multiply by 100 and enter the results in column 4 The values of column 4 should all fa...

Страница 28: ...lty evaluation result remains continue below 6 6 1 Faulty gradient The gradient is linear but the interval is too small Mixer chamber too large or faulty mixer Disturbances may arise from air in the p...

Страница 29: ...response table 7 3 UV response test result UV response table 1 Programmed Conc B 2 Value read 3 Baseline cor rected value 4 Normalised value 5 Allowed interval 100 95 94 96 70 69 71 30 29 31 5 4 6 0 1...

Страница 30: ...stalled pH electrode holder installed optional Computer and printer installed UniNet 1 cabling installed Mains power cabling installed 3 Installation test Solutions prepared Tubings to piston seal rin...

Страница 31: ...ate Note Fill in serial numbers over leaf IMPORTANT WARRANTY REGISTRATION INFORMATION Please ensure that this form is completed and returned to Service Administration to register the users equipment u...

Страница 32: ...on AC 9 1 Components KTAexplorer system serial numbers QTY Part Number Description Serial Number System rack Mixer M 925 Monitor UV 900 Pump P 900 pH C 900 INV 907 PV 908 SV 903 A SV 903 B Computer Co...

Страница 33: ...Registration form 9 KTAexplorer Installation Guide 18 1139 59 Edition AC 33 18113959AC_TK book Page 33 Monday June 23 2003 4 45 PM...

Страница 34: ...9 Registration form 34 KTAexplorer Installation Guide 18 1139 59 Edition AC 18113959AC_TK book Page 34 Monday June 23 2003 4 45 PM...

Страница 35: ...18113959AC_TK book Page 33 Monday June 23 2003 4 45 PM...

Страница 36: ...TC information Uppsala Printed in Sweden by TK i Uppsala AB 18113959AC_TK book Page 34 Monday June 23 2003 4 45 PM...

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