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In the Pulse type, Biphasic (biphasic pulse) or Const (monophasic pulse) is selected. Usually, select Biphasic. In Duration, the pulse
width is specified in 0.1 s increments. When Biphasic is selected, 50% of the specified value is applied to each phase. In Intensity, a
positive or negative integer is specified. When Biphasic is selected, the designated value is assigned to the first phase and the
value with an inverted sign is assigned to the second phase. In Pre and Post, the waiting time before and after a pulse is specified
in 0.1 s increments. For the reason why a biphasic pulse is selected, refer to p. 24 “6.2.3 Stimulus artifact and biphasic stimulation.”
Note: In order to return the output current of the stimulation pulse to the 0 level, be sure to set the Post value for the last pulse of
the stimulation pattern.
The Pulse config represents 1 pulse range of a stimulation pattern. A stimulation pattern is composed by clicking the buttons
shown below to add, delete or repeat 1 pulse range to the whole stimulation pattern.
Menus in EASY SETTINGS
Custom ….. When the software is closed while Custom is selected, all the defined data acquisition condition can be stored and
called up at the subsequent launching of the software. This menu is provided for the user’s convenience and users can
repeatedly use the specific data acquisition condition defined by themselves without having to define it again.
Note: In menus other than Custom, the condition defined by the user cannot be stored. Default values are called up
every time the software is launched.
EP - baseline ….. This menu is provided for recording the evoked response (the evoked potentials) in a brain slice. A session
consisting of 0.1 s data acquisition and 19.9 s waiting time is repeated 1,000 times. Max input is set at 10,000 uV
and the data in bandwidths 1 to 10,000 Hz are shown. For example, for an acute hippocampus slice, it is in good
condition if amplitude ≥-300 uV is obtained by a default stimulation pulse (0.2 ms width, -10 uA) (an estimated
value).
Pulse type
Electrode to which the stimulation pattern is applied
(it cannot be set for an individual pulse)
Pulse #
#1
#2
#3
#4
Pre
Post
Duration
Pre and post pulse
time
Pulse duration
Stimulus intensity
The pulse designated by Pulse # or selected
by a direct click on the STIMULATION
PATTERN panel is highlighted in red.
Pulse selected is deleted.
Specified number of a pulse
set in Pulse config is added
after the pulse selected.
Stimulation intensity specified in
Increase intensity is applied to all
units.
The stimulation intensity specified in
Increase intensity is accumulatively applied
for each repeat to all units.
This button is always on or off.
The stimulation pattern defined is
repeated continuously in a repeat.
This button is always on or off.
Intensity
Const
Biphasic
1st phase
2nd phase
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