Agilent Technologies SureCycler 8800, Руководство по установке и эксплуатации

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PCR Programs 4
Using a Preloaded PCR Program
Agilent SureCycler 8800 Setup and User’s Guide 51
Using a Preloaded PCR Program
Several PCR programs come preloaded into the software for use on the
96- well SureCycler 8800. These programs were designed for performing
PCR with a variety of Agilent PCR enzymes and kits to amplify human
genomic DNA or vector DNA.
Each preloaded program is optimized to amplify targets of a particular
length with an annealing temperature that is suitable for some primers.
You may need to adjust the annealing temperature, the elongation time or
another cycling parameter used in the preloaded program to better suit
your particular target of interest. Any of the preloaded programs can be
saved under a new file name and then edited to optimize the program for
your primer/template system.
The available preloaded programs are listed in Table 5 .
Table 5 Description of the preloaded PCR programs for the 96-well SureCycler 8800
PCR Program File Name PCR Enzyme or Kit Denaturation Annealing Elongation
Herculase II Fusion 1kb target.pcr Herculase II Fusion DNA Polymerase 95°C for
20 seconds
55°C for
20 seconds
72°C for
30 seconds
Paq5000 HS MM 1kb target.pcr Paq5000 Hotstart Master Mix 95°C for
20 seconds
55°C for
20 seconds
72°C for
30 seconds
PfuTurbo HS MM 1kb target.pcr PfuTurbo Hotstart Master Mix 95°C for
30 seconds
55°C for
30 seconds
72°C for
1 minute
PfuUltra II HS MM 1kb target.pcr PfuUltra II Hotstart Master Mix 95°C for
20 seconds
55°C for
20 seconds
72°C for
15 seconds
QuikChange II 4 kb target.pcr QuikChange II Site-Directed
Mutagenesis Kit 95°C for
30 seconds
55°C for
1 minute
68°C for
4 minutes
QuikChange Lightning 4kb target.pcr QuikChange Lightning Site-Directed
Mutagenesis Kit 95°C for
20 seconds
60°C for
10 seconds
68°C for
2 minutes
QuikChange Lighting Multi 4 kb
target.pcr QuikChange Lightning Multi
Site-Directed Mutagenesis Kit 95°C for
20 seconds
55°C for
30 seconds
65°C for
2 minutes
QuikChange Multi 4kb target.pcr QuikChange Multi Site-Directed
Mutagenesis Kit 95°C for
1 minute
55°C for
1 minute
65°C for
8 minutes