Agilent Technologies Seahorse XFp, Руководство пользователя

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Agilent Seahorse XFp Glycolytic Rate Assay Kit
User Guide
Agilent Technologies
2
Kit Information
Kit Contents 11
Kit Shipping and Storage 11
Additional Required Items 12
Kit Contents
The Seahorse XFp Glycolytic Rate Assay Kit includes six foil
pouches, each containing Rot/AA mix and 2-DG. The kit
reagents are sufficient for six complete XFp Glycolytic Rate
Assays in an Agilent Seahorse XFp Cell Culture Miniplate.
Kit Shipping and Storage
The product ships at ambient temperature, and should be
stored at room temperature. The product is stable for one year
from the date of manufacture. The actual expiration date is
printed on the label of the assay kit box. Depending on the
shipping date, the actual shelf life of the kit in user’s hand can
vary between 12 to 3 months.
Table 1 Agilent Seahorse XFp Glycolytic Rate Assay kit contents in each foil pouch

Compound

Target

Effect
Cap
color Quantity per
tube
Rotenone plus
Antimycin A (Rot/AA) Mitochondrial ETC
complexes I and III,
respectively Inhibits mitochondrial respiration,
typically leading to an increase in
glycolysis Red 5.4 nmol each
2-deoxy-D-glucose (2-DG) Hexokinase (rate-limiting
enzyme in glycolysis) Inhibits hexokinase, leading to a
decrease in glycolysis Green 150 µmol