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116 1200 Series Handheld Control Module Users’s Guide
9 Running an Isocratic Analysis
Entering Settings
Entering Settings
To set up the isocratic analysis you will set the LC system settings to default
and then modify selected settings, the other settings will remain with their
default values. You will then save these settings to a method called ISO.
1
Enter the
Analysis
screen.
2 Set the vial range as 1 to 1.
3
Set the number of injections to 1.
4
Press Enter to skip the method name section.
5 Select the Settings button [ F1 ].
6
Select LC System from the menu.
7
Select the
Default
button [ F7 ] and select the
Yes
button to load defaults.
8 Press Esc .
9
Select the
Settings
button [
F1
].
10
Select the pump from the menu (either ISO, BIN or QUAT).
11 Press the Pressure button [ F3 ].
12
Enter 400 as the upper pressure limit.
13
Select the
Done
button [ F6 ].
14 Enter the following values: %B 65, (%C OFF, %D OFF for quarternary pump.
If you have a binary pump, set %B to 65.).
15 Set the Flow to 1,5 ml/min.
16
Confirm by selecting “
Done
” to enter the
Analysis
screen
17
Enter the values shown in Table 4 in the
Analysis
screen.
Table 4 Values in Analysis Screen
Setting Value
Detection wavelength 254 nm
Injection volume 1 µl
Stoptime 6 min