Maintenance and Spare Parts
Cary Eclipse Fluorescence Spectrophotometer User’s Guide
35
Aligning
For optimum performance of the instrument, the lamp module must
be aligned.
To align the lamp module:
NOTE
The instrument power must be on for the alignment procedure.
1
Open the Cary Eclipse folder on the desktop and then double-
click
Align
to start the ‘Align’ application.
2
Remove the lamp access panel (see Figure 1) to access the lamp
alignment screws.
3
In the Align application, click the
Cary
tab and set up these
instrument parameters:
Ex. Wavelength (nm): 540
Ex. Slit (nm): 1.5
Ave. time (s): 0.1
PMT voltage: Medium
NOTE
Emission parameters are not important for this procedure.
4
Click the
Graph
tab.
5
View the reference signal by clicking the
Reference Signal
button
on the toolbar.
6
Click the
Vertical Rescale
button to find the signal range.
7
Click the
Scale
button and enter an upper limit that is 20%
higher than the current signal and a lower limit that is 20% lower
than the current signal. This will make the adjustment easier
when observing the graph.
8
Adjust the bottom left screw (vertical adjustment, labeled ‘1’ in
Figure 5) for maximum signal with the supplied 5.5 millimeter
nut driver (you may need to adjust the y-axis scaling on the
screen).
