3025 SERIES PHASE CONTRAST SET-UP INSTRUCTIONS
1.
Place the microscope in front of you and plug in your light source.
2.
Mount the objectives clockwise (lowest magnification to highest magnification) onto the
microscope nosepiece.
3.
Install the turret phase condenser (Fig. 2) into the condenser holder located under the
stage and tighten into place.
4.
Position the turret phase condenser so that the “BF” on the rotatable annuli selector wheel
is facing you. This places the condenser iris diaphragm in the light path. Rack up the
condenser to the top and open the diaphragm.
5.
Place a specimen slide on the stage and focus on it using the 10X objective.
6.
Shift the slide to an area where the specimen is no longer visible; however, do not change
the focus.
7.
Rotate the phase turret wheel counter-clockwise until the “10” figure clicks into position.
Note: There are two rotating wheels located at the front of the phase condenser. They are
used to center the annuli in the condenser or phase rings.
8.
Remove one of the eyepieces of the microscope and replace it with the phase telescope
(Fig. 3). Note: The upper collar of the phase telescope can be moved in or out allowing
you to bring the phase rings into focus.
9.
Looking through the phase telescope you will see a dark field of view with two different
rings ( a & b), one bright (white) and the other black.
10.
While viewing the rings through the phase telescope, adjust the two rotating wheels from
the turret phase condenser until the narrower white ring is superimposed equidistant
within the black ring (c).
11.
Remove the phase telescope from the microscope and replace the eyepiece.
12.
Bring the specimen back into the field of view and refocus the image until it is sharp. The
image will be detailed and exhibit fine structural information.
NOTE: You must re-center the phase rings every time you change magnification by using a
different objective. The turret wheel must be rotated so its number corresponds with the
objective being used. To return to brightfield viewing, rotate the turret wheel until it is at the
“BF” position.
