3012 Microscope
Phase Contrast
– Turret System Instructions
Installation of the Phase Contrast Components
& Alignment of the Condenser to the Optic Axis of the Microscope
1.
Mount the Phase Contrast objectives onto the nosepiece
— clockwise lowest to highest magnification.
2.
Install the Phase Contrast Turret-style condenser onto the condenser carrier.
3.
Raise the condenser carrier rack to its highest position.
4.
Position the condenser turret rotating plate to the “BF” position. (Make certain the condenser diaphragm is full
open).
5.
Select the 10x Phase Contrast objective.
6.
Place a “stained slide” specimen on the stage and focus the microscope.
7.
Close the field diaphragm, then lower the condenser until an image of the field diaphragm comes into focus on the
object plane.
8.
Partially open the field diaphragm until the image of the diaphragm is close to the edge of the field of view. Now
adjust the condenser carrier “centering screws” to align the condenser to the microscope optical axis.
9.
Replace the “stained slide” with a phase contrast specimen (for example, a fresh “cheek cell” preparation).
10. Remove one eyepiece and install the supplied alignment telescope. Focus the telescope on the phase ring inside
the 10x Phase Contrast objective.
11. Rotate the condenser-
turret to its “10/20” position. As you observe through the telescope you will see two different
rings, See illustration
Phase Plate and Light Annulus Alignment.
12. The condenser-turret has two adjusting screws. These are used to align annular light rings in the condenser-turret
to the corresponding phase ring in the objectives. Adjust the 10/20 annulus so that it is aligned
– see Figure C
below. (The 10/20 annulus will now function with
both
the 10x and optional 20x Phase objective).
13. Repeat the above annulus alignment procedure for the 40
x
and optional100x Phase objectives.