TROUBLESHOOTING GUIDE
PHASE CONTRAST MICROSCOPY
PROBLEM
CAUSE
CORRECTIVE MEASURE
Poor phase
contrast image
is obtained
The condenser phase annulus image
and the objective phase plate are not
aligned
Adjust the phase annulus so that it is
aligned with the objective phase
plate.
The condenser phase annulus and the
objective phase code do not match.
Rotate the phase annulus selector
wheel to the position that matches the
objective in use
The phase difference of the specimen
is too large.
Prepare the specimen using a
different refractive index immersion
fluid
The specimen cover glass is incorrect
Replace with 0.17mm thick cover
glass
Содержание 3000-LED series
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