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CELL-DYN
®
3200 System Operator’s Manual
6-35
9140181H—October 2001
Section 6
Calibration Procedures
WOC, NOC, RBC, and PLT
Reference values for white blood cells, red blood cells, and platelets may be
determined using multiple counts from a certified hemocytometer, from a counter
that meters a fixed, calibrated sample volume, or from a reliably calibrated
hematology analyzer.
HGB
Reference values for hemoglobin may be determined using either the reference
cyanmethemoglobin method or a reliably calibrated hemoglobinometer or
hematology analyzer.
NOTE: DO NOT
attempt to calibrate the CELL-DYN 3200 System with a
hemoglobin standard designed for the calibration of specific reference
cyanmethemoglobin methods. The instrument uses a modified
hemoglobinhydroxyalamine method which is not designed to analyze
these standards directly.
MCV
Reference values for the mean cell volume may be determined by calculation from
the reference microhematocrit and RBC measurements or from multiple analyses
on a reliably calibrated hematology analyzer.
NOTE:
Reference microhematocrit values may be determined by multiple
analyses using the NCCLS method for Packed Cell Volume (PCV).
2
Use
only plain (non-anticoagulated) capillary tubes. Be certain to verify the
proper operation of the microhematocrit centrifuge and the timer as
recommended by NCCLS.
Determining Reference Values — Fresh Whole Blood
Follow the procedures below to determine the reference values that will be used to
calibrate the instrument using fresh whole blood.
NOTE:
No more than two hours should elapse between determining the
Reference Mean Values and performing the calibration.
WARNING: Potential Biohazard.
Consider all specimens potentially
infectious. Wear gloves, lab coats, and safety glasses and follow other
biosafety procedures as specified in the OSHA Bloodborne Pathogen Rule
(29 CFR 1910.1030) or other equivalent biosafety procedures.
1. Go to a reference hematology instrument (or use appropriate hematology
methods) with 5 samples of normal, fresh whole blood. Run each sample at
least twice for a minimum of 10 replicates on the reference instrument.
NOTE:
Because the same samples will be used to first obtain reference
values on a reference instrument then to calibrate in the Open and
Closed Modes, it is important to begin with a sufficient amount of
each sample.
Содержание CELL-DYN 3200
Страница 12: ...xii CELL DYN 3200 System Operator s Manual 9140181K July 2002 NOTES ...
Страница 52: ...1 4 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Use or Function Overview Section 1 NOTES ...
Страница 132: ...3 38 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Principles of Operation References Section 3 NOTES ...
Страница 154: ...5 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Operating Instructions Overview Section 5 NOTES ...
Страница 234: ...5 82 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Operating Instructions Run Menu Section 5 NOTES ...
Страница 300: ...6 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Calibration Procedures Overview Section 6 NOTES ...
Страница 356: ...6 58 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Calibration Procedures References Section 6 NOTES ...
Страница 362: ...8 2 CELL DYN 3200 System Operator s Manual 9140181K July 2002 Hazards Overview Section 8 NOTES ...
Страница 370: ...8 10 CELL DYN 3200 System Operator s Manual 9140181K July 2002 Hazards References Section 8 NOTES ...
Страница 372: ...9 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Service and Maintenance Overview Section 9 NOTES ...
Страница 532: ...11 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Quality Control Overview Section 11 NOTES ...
Страница 558: ...11 28 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Quality Control References Section 11 NOTES ...
Страница 560: ...12 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Printers Overview Section 12 NOTES ...
Страница 566: ...13 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Sample Loader Overview Section 13 NOTES ...
Страница 590: ...14 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Reticulocyte Package Overview Section 14 NOTES ...
Страница 662: ...A 4 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Bar Codes Overview Appendix A NOTES ...
Страница 666: ...A 8 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Bar Codes Specifications Appendix A NOTES ...