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CELL-DYN
®
3200 System Operator’s Manual
3-21
9140181H—October 2001
Section 3
Principles of Operation
MCH
The
Mean Corpuscular Hemoglobin
is the average amount of hemoglobin
contained in the red blood cell expressed in picograms. The MCH is calculated
from the RBC and the HGB as follows:
MCH = (HGB/RBC) x 10
MCHC
The
Mean Corpuscular Hemoglobin Concentration
is the ratio of the weight of
hemoglobin to the volume of the average red blood cell expressed in grams per
deciliter. MCHC is calculated from the HGB and the HCT as follows:
MCHC = (HGB/HCT) x 100
RDW
Red Cell Distribution Width
is a measure of the heterogeneity of the RBC
population. The CELL-DYN 3200 reports a relative RDW equivalent to a CV in
grams per deciliter. The RDW is derived from the RBC histogram using the 20th
and 80th percentiles.
RBC Flagging
Refer to the “Operational Messages and Data Flagging” subsection of this section
for RBC Flagging information.
Platelet Parameters
Events counted in the RBC/PLT dilution between floating thresholds are included
in the platelet (PLT) data, which is collected using the 0° and 10° sensors. The
lower threshold floats between 1 and 3 fL and the upper threshold floats between
15 and 35 fL. If there is not enough data to determine the PLT count, the lower and
upper thresholds are set at 2 and 35 fL respectively. Once the thresholds have been
determined, the PLT count is derived from the 10° data.
Data can be displayed in two formats. It can be displayed as a scatterplot (0°/10°)
including the RBCs. Data can also be displayed as one of the following three
histograms:
PLT only using 10° data
PLT and RBC using 0° data
PLT and RBC using 10° data
PLT data are shown as a histogram of the 10° data in the following figure.
Events counted in the region below the lower threshold are usually either optical
noise or small particulate matter. Events counted in the region above the upper
threshold are counted as RBCs. If interference with either threshold region exceeds
a predetermined limit, the PLT parameters are flagged accordingly. The flags are
discussed in the last section of this chapter.
Содержание CELL-DYN 3200
Страница 12: ...xii CELL DYN 3200 System Operator s Manual 9140181K July 2002 NOTES ...
Страница 52: ...1 4 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Use or Function Overview Section 1 NOTES ...
Страница 132: ...3 38 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Principles of Operation References Section 3 NOTES ...
Страница 154: ...5 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Operating Instructions Overview Section 5 NOTES ...
Страница 234: ...5 82 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Operating Instructions Run Menu Section 5 NOTES ...
Страница 300: ...6 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Calibration Procedures Overview Section 6 NOTES ...
Страница 356: ...6 58 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Calibration Procedures References Section 6 NOTES ...
Страница 362: ...8 2 CELL DYN 3200 System Operator s Manual 9140181K July 2002 Hazards Overview Section 8 NOTES ...
Страница 370: ...8 10 CELL DYN 3200 System Operator s Manual 9140181K July 2002 Hazards References Section 8 NOTES ...
Страница 372: ...9 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Service and Maintenance Overview Section 9 NOTES ...
Страница 532: ...11 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Quality Control Overview Section 11 NOTES ...
Страница 558: ...11 28 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Quality Control References Section 11 NOTES ...
Страница 560: ...12 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Printers Overview Section 12 NOTES ...
Страница 566: ...13 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Sample Loader Overview Section 13 NOTES ...
Страница 590: ...14 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Reticulocyte Package Overview Section 14 NOTES ...
Страница 662: ...A 4 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Bar Codes Overview Appendix A NOTES ...
Страница 666: ...A 8 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Bar Codes Specifications Appendix A NOTES ...