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CELL-DYN
3000 System Operator’s Manual
3-9
9140240E — May 1995
Chapter 3
Principles of Operation
The light source is a vertically polarized 5 mW helium-neon laser with a
wavelength of 632.8 nm. The laser beam passes through a cylindrical
lens which changes the shape from a circle to an ellipse. The beam is
then directed through a 125 µm slit which blocks the weaker outer edges.
This process yields a uniformly intense beam approximately 80 µm wide.
Consequently, the cell stream may wander slightly in the flow cell and
yet still be exposed to the same light intensity. An imaging lens centers
the focused laser beam onto the quartz flow cell.
The WBC metering syringe slowly injects 78 µL of the WBC dilution
into the sheath stream in the WBC flow cell. The sample is
hydrodynamically focused into a small stream approximately 30 µm in
diameter. This focused stream aligns the diluted cells in single file as
they pass through the sensing region, which allows them to be analyzed
one at a time.
Figure 3.3:
Optical Bench
Since the average WBC is much smaller than the focused laser beam, the
cells do not scatter much laser light. If the remaining so-called axial light
were allowed to reach the 0
°
detector, it would saturate the electronics.
Therefore, it is blocked from the detector by the obscuration bar. The
forward angle scatter is directed to a perforated mirror. The 0
°
light
scatter passes through the mirror to the 0
°
photodiode detector. The 10
°
light scatter is deflected off the mirror to the 10
°
photodiode detector.
90
°
Light
Scatter
PMT
90
°
Depolarized
Light Scatter
PMT
Polarizer
(Horizontal)
Helium-Neon Laser
(632.8 nm) Polarized
Vertically
Beam
Splitter
10
°
Light
Scatter
Photodiode
0
°
Light
Scatter
Photodiode
Perforated
Mirror
Obscuration
Bar
WBC
Flow
Cell
Imaging
Lens
Front
Surface
Mirror
Cylindrical
Lens
700
µ
m
Slit
Front
Surface
Mirror
125
µ
m
Vertical
Slit
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Содержание CELL-DYN 3000
Страница 31: ...10 CELL DYN 3000 System Operator s Manual 9140240E May 1995 NOTES Search Go Back Book TOC ...
Страница 265: ...6 10 CELL DYN 3000 System Operator s Manual 9140240E May 1995 Calibration Chapter 6 NOTES Search Go Back Book TOC ...
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Страница 351: ...7 2 CELL DYN 3000 System Operator s Manual 9140240E May 1995 Quality Control Chapter 7 NOTES Search Go Back Book TOC ...
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Страница 371: ...7 22 CELL DYN 3000 System Operator s Manual 9140240E May 1995 Quality Control Chapter 7 NOTES Search Go Back Book TOC ...
Страница 387: ...9 6 CELL DYN 3000 System Operator s Manual 9140240E May 1995 Maintenance Chapter 9 NOTES Search Go Back Book TOC ...
Страница 405: ...9 24 CELL DYN 3000 System Operator s Manual 9140240E May 1995 Maintenance Chapter 9 NOTES Search Go Back Book TOC ...
Страница 431: ...10 2 CELL DYN 3000 System Operator s Manual 9140240E May 1995 Troubleshooting Chapter 10 NOTES Search Go Back Book TOC ...
Страница 473: ...10 44 CELL DYN 3000 System Operator s Manual 9140240E May 1995 Troubleshooting Chapter 10 NOTES Search Go Back Book TOC ...
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Страница 535: ...12 16 CELL DYN 3000 System Operator s Manual 9140240E May 1995 Sample Loader Chapter 12 NOTES Search Go Back Book TOC ...
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