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CELL-DYN
3000 System Operator’s Manual
9140240E — May 1995
Quality Control
Chapter 7
X-B Analysis
Introduction
X-B analysis is an automated means of monitoring instrument
performance by using the known stability of the red cell indices.
X-B is used to indicate X
B
, which is the symbol for a moving average of
hematology values calculated using an algorithm developed by Dr. Brian
Bull of Loma Linda University. X-B analysis uses the Bull algorithm to
monitor instrument performance by tracking the average red cell indices
in the patient population analyzed on the instrument.
The red cell indices, MCV, MCH and MCHC, are known to be stable
because the red cell apparently functions best in a very narrow range of
size and Hemoglobin content. Therefore, the body exerts tight
physiologic control and will vary the number of red cells before altering
the average volume or Hemoglobin concentration of those red cells.
Consequently, the
average red cell indices of a given patient population
will vary no more than 0.5 percent from day to day and even year to year,
providing the population does not change
a means of utilizing this information for quality control on the
CELL-DYN 3000.
The X-B algorithm analyzes the indices on the patient samples run
through the instrument in batches of 20. The mean of each batch is
compared to a target value, and a percent deviation is computed and
compared to the acceptable limits. This is similar to comparing the
results of a commercial control run to the appropriate assay value to
determine whether the result falls within the 2SD range.
Lower/Upper Acceptance Limits
The lower and upper limits determine which patient results will be used
in the X-B moving average calculation. They should be set wide enough
to exclude grossly abnormal samples (such as background counts) but
should include at least 95% of the patient results. Only results that fall
within the set limits are used in the calculation.
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