Abaxis VetScan HM5, Руководство оператора

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Running a Sample 4-3

When using a vacutainer system, allow the vacuum to pull the sample in. If the tube stops
filling before enough sample is acquired, try to quickly redirect the needle.
If this takes more than 5-10 seconds, remove the needle and dispose of the sample, and
attempt a new draw at a new site, with a new needle and new tube. Once the tube is full,
mix by inverting the tube 10-15 times (25-30 times in low volume microtubes).

When using a butterfly with a vacutainer system, gently invert the tube while the tube is
filling.

IMMEDIATELY mix tubes after filling them to prevent the blood from clotting in the
tube.
4.2 Storing Samples

Samples may be put in a rack or on a counter until ready to run.

Samples should be run within 4 hours if stored at room temperature, or 8 hours if refrig-
erated.

If the samples were refrigerated, allow them to warm to room temperature before analy-
sis by sitting on the counter for 10-15 minutes. Samples may also be warmed by slowly
rolling the tube in the palms of the hands for 5-10 minutes.

Do not use a rocker to mix samples smaller than 1.0 ml.
4.2.1 Remixing Before Running
The HM5 software will display a reminder to mix samples before each sample run.

Always mix the sample again with 10-15 inversions before analyzing the sample in the
HM5. Over time, samples settle and start to separate so they must be mixed well again
before being run.

If any specks of solid blood (indicating clotting) are observed, or if there were any delays
in filling the tube, discard the sample and redraw.
Note: Rockers do not mix samples well. To mix properly, invert each sample
by hand 10 to 15 times immediately after drawing, then invert 10 to 15
times again immediately before running the sample.