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4titude Ltd, The North Barn, Surrey Hills Business Park, Damphurst Lane, Wotton, Dorking, Surrey, RH5 6QT, UK
Tel: +44 (0) 1306 884885 Fax: +44 (0) 1306 884886 [email protected] www.4ti.co.uk

Page | 32

SEALING PARAMETERS

The following sealing temperatures are for guidance only. Sealing
efficiency varies depending on the plate type used.

Temperature (°C) / Dwell Time (secs)

Part

number

Description

96-well

PCR Plates*

384-well

PCR Plates*

Vision

Plates™

4ti-0541 Clear Seal

175-185 / 2-3 165-180 / 3 185-200 / 3

4ti-0575 Clear Weld Seal Mk II

175-180 / 2-3 170-175 / 2-3

4ti-0581 Clear Seal 3730

165-175 / 3

165-175 / 2 175-185 / 2-3

4ti-0521 Peel Seal

175-185 / 3 170-175 / 2-3

4ti-0587 DMSO Resistant Peel Seal

175-185 / 3 170-175 / 2-3

4ti-0531 Pierce Seal

160-175 / 2

160-175 / 2 185-200 / 3

4ti-0536 Foil Seal

165-180 / 2 160-175 / 2-3 185-200 / 3

4ti-0547 PS Foil Seal

185-200 / 3

4ti-0527 Black Seal

185-200 / 3

4ti-0591 Thermal Bond

170-180 / 2-3 160-170 / 2

4ti-0595 Gas Permeable Heat Seal

165-175 / 3

165-175 / 3 175-185 / 3

Table 2 - Sealing Temperatures and Times for different types of plates

* These values are for two-component PCR plates. For one-piece polypropylene
plates, increase dwell time by one second.

These values can be used for all polystyrene plates

All sealing parameters were estimated using software version 3.03

These values are for guidance only. It is advisable to conduct trials
depending on the plate type being sealed.

Add 1 second to the sealing time when a weighted platen is

used (4ti

-0602)

Содержание 4s2

Страница 1: ...4s2 Variable Temperature Thermal Sealer Version M Software Version 3 03 11 October 2011 ...

Страница 2: ... means electronic mechanical magnetic optical chemical or other without the prior written permission of the manufacturer The manufacturer reserves the right to revise this publication and to make changes from time to time in the contents hereof without obligation to notify any person of such revision or changes Unless otherwise specified references to names or parts are purely casual and are used ...

Страница 3: ... product code 4ti 0650 4ti 0650 4s2 Heat Sealer Power Cable Manual Quick User Guide 4ti 0615 Plate Carrier 4ti 0602 Weighted Platen Replacement accessories and optional extras are to be ordered separately 4ti 0625 Plate carrier for 96well PCR Plates optional extra 4ti 0612 Sealing Frame supplied FOC with 4ti 0625 4ti 0616 Roche 1536 Plate Adapter optional extra 4ti 0626 Warranty extension 1year op...

Страница 4: ...4titude Ltd The North Barn Surrey Hills Business Park Damphurst Lane Wotton Dorking Surrey RH5 6QT UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 3 ...

Страница 5: ...echnical Specifications 10 2 GENERAL SPECIFICATION 12 2 1 Sealer Parts 12 2 1 1 Control Panel 14 2 1 2 Rear Power Interface 14 3 INSTALLATION 15 3 1 Unpacking and start up 15 3 2 Voltage Check 16 4 SET UP 18 4 1 Set Up Operating Mode 18 4 2 Stand By Power Down and Audible Warning Control 19 5 BASIC FUNCTIONS 20 5 1 Run Mode 20 3 5 2 Stand by Mode 23 5 3 Power down Mode 24 5 4 Drawer Locking 24 6 T...

Страница 6: ...884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 5 7 MAINTENANCE 29 7 1 Regular Maintenance 29 8 ACCESSORIES AND CONSUMABLES 30 8 1 Plate Types 30 8 2 Thermal Sealing Plastic and Sealing Film Types 30 8 3 Plate Carriers and Accessories 31 9 SEALING PARAMETERS 32 10 SHIPPING INSTRUCTIONS 33 11 WARRANTY 34 ...

Страница 7: ...e plate carrier The sealing operation starts once the drawer with the plate carrier has been closed Film and sample plate will then automatically be brought in contact with the heating platen As soon as the sample plate is in contact with the heating platen a countdown timer starts and after the chosen sealing time the motor automatically moves the sample plate away from the heating platen and the...

Страница 8: ...4titude Ltd The North Barn Surrey Hills Business Park Damphurst Lane Wotton Dorking Surrey RH5 6QT UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 7 ...

Страница 9: ...mperature for a considerable time after switching off and it is essential to wait before starting cleaning operations Sealed sample plates may remain hot for some seconds after sealing and should be handled with care WARNING Risk of electrical shock high voltage This equipment should only be dismantled by properly trained personnel removing the top case exposes potentially lethal mains voltages 1 ...

Страница 10: ...4titude Ltd The North Barn Surrey Hills Business Park Damphurst Lane Wotton Dorking Surrey RH5 6QT UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 9 ...

Страница 11: ...nfo 4ti co uk www 4ti co uk Page 10 1 5 Technical Specifications MECHANICAL Dimensions Height 310 mm Depth 275 mm Width 181 mm Weight 9 kg ELECTRICAL Voltage 230 115 100 10 Vac Frequency 47 63 Hz Power 300 W ENVIRONMENTAL Temperature range Operative 18 C to 35 C Storage 20 C to 40 C Relative humidity 20 to 80 not condensing SOFTWARE Version 3 03 ...

Страница 12: ... OFF or from 120 C to 200 C Step 1 C Default OFF Sealing time Range 0 to 10 seconds Step 0 1 second 0 1 second Step 1 10 seconds 0 5 second Default 3 seconds Stand by cycle Range no stand by or from 1 to 99 mins Step 1 minute Default 60 minutes Stand by temperature 60 C not programmable Power down time Range 1 to 24 hours Step 1 hour Default 10 hours Power down temperature Ambient Audible Warning ...

Страница 13: ...ane Wotton Dorking Surrey RH5 6QT UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 12 2 GENERAL SPECIFICATION 2 1 Sealer Parts Figure 1 Casing and Control Panel Figure 2 Rear Power Interface Figure 3 Heating Platen Figure 4 Plate Carrier ...

Страница 14: ...6QT UK Tel 44 0 1306 884885 Fax 44 0 1306 884886 info 4ti co uk www 4ti co uk Page 13 Figure 5 96well PCR Plate Adapter 4ti 0625 optional Figure 6 Standard Plate Adapter 4ti 0615 Figure 7 Roche 1536 Plate Adapter 4ti 0616 Figure 8 Weighted Platen 4ti 0602 Figure 9 Sealing Frame 4ti 0612 optional ...

Страница 15: ... 1306 884886 info 4ti co uk www 4ti co uk Page 14 2 1 1 Control Panel Figure 10 Control Panel Set up Run Mode Key Directional up Key Directional down Key Three digit LED display Amber LED Status Indicator 2 1 2 Rear Power Interface Figure 11 Rear Interface Power Setting 115 230 Vac and Fuse Holder I O Power Switch Mains Power Plug ...

Страница 16: ... from its packaging Open the sealing drawer and remove the protective cardboard The unit has to be placed on a level surface away from direct sunlight ensuring access to the power switch at the back of the unit Ensure that the vents on the casing are not obstructed The mains voltage selector switch should be checked to ensure that the voltage has been set at the correct value Connect the unit to t...

Страница 17: ...nt is not connected to the mains power At the rear of the 4s2 is the connection panel with Power setting 115 230 Vac and Fuse Holder I O Power Switch Mains Power Plug The set voltage is displayed in white on red background on the power setting and fuse holder see Figure 11 page 14 Figure 12 Opening fuse holder compartment Figure 13 Levering out fuse holder Figure 14 Correct fuse location Figure 15...

Страница 18: ...older compartment with a screwdriver Figure 12 2 With the screwdriver lever out the fuse holder Figure 13 3 Replace the two fuses according to the table below Power Voltage EN 60127 115V 3 15A T 230V 2A T Table 1 Fuse ratings for 4s2 4 Insert the fuse holder keeping the set voltage label at the top 5 Close the fuse compartment by pressing gently 6 If the fuse holder is inserted correctly you can r...

Страница 19: ...hilst it is heating up To programme time and temperature settings Briefly press and release the M key The display shows the last set sealing temperature flashing The temperature can be decreased or increased using the keys Temperature can be set between 120 C and 200 C with steps of 1 C or to oFF Press the M key again to store the new set temperature and then the display will flash with t and the ...

Страница 20: ...th steps of 1minute or as no standby Press the M key again to store the new stand by time and then the display will flash with P and the current power down time in hours The power down time is the time after which the heater is switched off automatically when the unit is left idle The power down time can be decreased or increased with the keys Power down can be set between 1 hour and 24 hours with...

Страница 21: ...lluminated The system is ready for sealing and the sample plate can be loaded onto the appropriate plate carrier Deep Well Plates All fully skirted SBS standard plates except 96well PCR plates can be loaded onto the standard plate carrier 4ti 0615 Figure 16 Figure 16 Use the standard plate carrier 4ti 0615 for deep well plates Shallow Well Plates However for easier handling of shallow well plates ...

Страница 22: ... the standard plate carrier should be removed from the drawer Figure 18 Plate adapter for 96well PCR plates optional code 4ti 0625 Figure 19 Plate adapter for 96well PCR plates shown with non skirted PCR plate Roche 1536well Plates For use with a Roche 1536 plate insert the Roche 1536well plate adapter 4ti 0616 inside the adapter for 96well PCR plates optional extra code 4ti 0625 Figures 20 21 Fig...

Страница 23: ...575 use the sealing frame which is supplied with the 4ti 0625 adapter For deep well plates place the weighted platen on top of the sealing film Figure 21 For most other plates we recommend the use of the sealing frame Figure 22 Figure 21 Position sealing film foil on plate Figure 22 Position weighted platen over sealing film foil Figure 23 Position sealing frame over sealing film foil Push the dra...

Страница 24: ...r and remove the sealed plate The unit will emit an audible beep as soon as the heating plate has reached the programmed temperature again and a new cycle can be started within a few seconds Note Foil backed film may remain hot for a number of seconds after sealing Care should be taken in handling these plates 5 2 Stand by Mode If left idle for a prolonged time the sealer will automatically switch...

Страница 25: ...hes 45 C after this LO will be displayed By pressing any key the unit will automatically begin to warm up the heating platen to the pre selected sealing temperature Refer to section 4 2 to change the time after which the unit switches into power down mode 5 4 Drawer Locking It is possible to lock the when the unit is not used to prevent damage or dust contaminating the internal parts of the unit T...

Страница 26: ... will be emitted at the same time Display Message Error Er1 The system cannot reach the starting position within a defined time Er2 The lifting platform does not remain in the elevated position Er3 The motor cannot move the lifting platform towards the heating plate within a defined time Er4 The motor cannot move the lifting platform to the low position within a defined time Er5 Motor over current...

Страница 27: ...generated the error e g error 7 may occur if the drawer is pushed in but released before the motor sets in motion To solve the problem please push in the drawer again and hold until an audible warning is heard If any of the above errors persists switch OFF the unit and then switch ON again If within 10 seconds the problem is not solved the heated plate is automatically turned off to avoid sample d...

Страница 28: ...g film upside down on the microplate or PCR plate the sealing film may get stuck on the heated sealing plate To clean it proceed as follows Switch OFF the unit Disconnect the unit from the mains power supply Release the lever on the back of the unit counterclockwise see Figure 24 Figure 25 Lever in locked position The drawer can now be fully removed from the unit Do not attempt to clean the unit u...

Страница 29: ...move drawer Peel off the sealing film from the heater platen inside the unit by hand and clean with a paper towel if required Note The cleaning operation is much easier when the heater platen is near ambient temperature Reinsert the drawer into the unit Move the lever on the back of the unit clockwise to secure the drawer mechanism see Figure 26 Figure 27 Lever in open position Switch unit on ...

Страница 30: ... cleaned with a cloth dipped in water or ethanol methanol formaldehyde can also be used The unit should not be immersed in solvents Do not use acetone or abrasive cleaners No parts are to be autoclaved In case of radioactive spillages it is recommended to use an appropriate cleaning agent For any other maintenance operation not indicated in this manual please contact 4titude Any cleaning procedure...

Страница 31: ...MABLES The 4s2 is designed to be used with the following consumables 8 1 Plate Types Any of the following made from polypropylene polyethylene or polystyrene SBS format shallow well plates Deep well plates Tube blocks various PCR plates 8 2 Thermal Sealing Plastic or Sealing Film types Foil polypropylene laminate Clear polyester polypropylene laminate Clear polymer Thin clear polymer Foil laminate...

Страница 32: ...luded product code 4ti 0615 see page 13 Figure 6 Plate carrier for 96well PCR plates optional extra product code 4ti 0625 see page 13 Figure 5 Roche 1536 Plate Adapter optional extra product code 4ti 0616 see page 13 Figure 7 Weighted Platen to hold down sealing sheets included code 4ti 0602 see page 13 Figure 8 Sealing Frame to hold down sealing sheets included with plate carrier 4ti 0625 or use ...

Страница 33: ...0 175 2 3 4ti 0587 DMSO Resistant Peel Seal 175 185 3 170 175 2 3 4ti 0531 Pierce Seal 160 175 2 160 175 2 185 200 3 4ti 0536 Foil Seal 165 180 2 160 175 2 3 185 200 3 4ti 0547 PS Foil Seal 185 200 3 4ti 0527 Black Seal 185 200 3 4ti 0591 Thermal Bond 170 180 2 3 160 170 2 4ti 0595 Gas Permeable Heat Seal 165 175 3 165 175 3 175 185 3 Table 2 Sealing Temperatures and Times for different types of p...

Страница 34: ...rom the unit Note If left inside the unit the plate carriers may cause severe damage to the sealer 2 Switch off the unit 3 Pull out the drawer 4 Place card board sheet provided between the underside of the drawer and the top of the lifting platform inside the unit 5 Push drawer in 6 Replace cardboard insert in drawer Note if the unit is shipped without the cardboard insert in the drawer the front ...

Страница 35: ...th in the user documentation related to such software Used refurbished products that are sold As Is are not covered by warranty Additional coverage plans may be purchased If the manufacturer receives note of defects or non conformance during the warranty period the manufacturer will at its option repair or replace the affected product The manufacturer will provide at no additional charge parts and...

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