Waters ACQUITY Overview And Maintenance Manual Download

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ACQUITY QDa Detector

Overview and Maintenance Guide

715003956
Revision F

Summary of Contents for ACQUITY

Page 2: ...t consult the Waters website waters com Trademarks ACQUITY is a registered trademark of Waters Corporation Alliance is a registered trademark of Waters Corporation Connections INSIGHT is a registered trademark of Waters Corporation PEEKTM is a trademark of Victrex PLC POZIDRIV is a registered trademark of Phillips Screw Company QDa is a registered trademark of Waters Corporation RADARTM is a trade...

Page 3: ...nformation Contacting medium Information Internet The Waters Web site includes contact information for Waters locations worldwide Visit www waters com Telephone and fax From the USA or Canada phone 800 252 4752 or fax 508 872 1990 For other locations worldwide phone and fax numbers appear in the Waters Web site Conventional mail Waters Corporation Global Support Services 34 Maple Street Milford MA...

Page 4: ...itted with a diaphragm pump assume an additional 0 5 maximum leak into the laboratory atmosphere Warning To avoid exposure to toxic substances and biohazards from O ring leaks in the source exhaust system observe these precautions Replace the source O rings at intervals not exceeding one year Prevent chemical degradation of the source O rings which can withstand exposure only to certain solvents b...

Page 5: ...utions Do not remove the mass spectrometer s protective panels The components they cover are not user serviceable When the instrument is in Operate mode avoid touching the areas marked with the high voltage warning symbol To touch external areas marked with the symbol first put the instrument in Standby mode Hazards associated with removing an instrument from service Warning To avoid personal cont...

Page 6: ... required and the level of residual contamination permissible That authority must also prescribe the method of decontamination to be used and the appropriate protection for personnel undertaking the decontamination process You must handle items such as syringes fused silica lines and borosilicate tips used to carry sample into the source area in accordance with laboratory procedures for contaminat...

Page 7: ... quality control QC procedures and the guidelines presented in this section Applicable symbols The following symbols can be present on the device system or packaging Symbol Definition Manufacturer Date of manufacture Authorized representative of the European Community Confirms that a manufactured product complies with all applicable European Community directives or Australia EMC compliant Confirms...

Page 8: ...tion tool as part of an ACQUITY UPLC or UPC system Alliance and LC and SFC prep systems The ACQUITY QDa detector is not intended for use in diagnostic applications Calibrating To calibrate LC systems adopt acceptable calibration methods using at least five standards to generate a standard curve The concentration range for standards must include the entire range of QC samples typical specimens and ...

Page 9: ...truments requirements Group 1 products apply to intentionally generated and or used conductively coupled radio frequency energy that is necessary for the internal functioning of the equipment Class A products are suitable for use in all establishments other than residential locations and those directly connected to a low voltage power supply network supplying a building for domestic purposes There...

Page 10: ...horized representative Address Waters Corporation Stamford Avenue Altrincham Road Wilmslow SK9 4AX UK Telephone 44 161 946 2400 Fax 44 161 946 2480 Contact Quality manager June 15 2017 715003956 Rev F Page x ...

Page 11: ...device vii Applicable symbols vii Audience and purpose viii Intended use of the ACQUITY QDa detector viii Calibrating viii Quality control viii EMC considerations ix FCC radiation emissions notice ix Canada spectrum management emissions notice ix ISM classification ISM group 1 class A ix EMC emissions ix EC authorized representative x 1 Instrument features and operating modes 16 1 1 Operating mode...

Page 12: ...d refitting the source enclosure 28 3 7 1 Removing the source enclosure from the instrument 28 3 7 2 Fitting the source enclosure to the instrument 30 3 8 Maintaining the source components 31 3 8 1 Removing the sample cone assembly from the ion block 31 3 8 2 Replacing the entrance aperture seal and disc 34 3 8 3 Removing the ion block 34 3 8 4 Cleaning the source components 36 3 8 5 Refitting the...

Page 13: ...56 A Safety advisories 59 A 1 Warning symbols 59 A 1 1 Specific warnings 60 A 2 Notices 62 A 3 Bottles Prohibited symbol 62 A 4 Required protection 63 A 5 Warnings that apply to all Waters instruments and devices 63 A 6 Warnings that address the replacing of fuses 67 A 7 Electrical and handling symbols 68 A 7 1 Electrical symbols 68 A 7 2 Handling symbols 69 B Specifications 71 B 1 Physical specif...

Page 14: ... external source exhaust valve assembly KAB 91 D 5 1 Connecting the external source exhaust valve assembly KAB 91 D 5 2 Disconnecting the external source exhaust valve assembly 94 D 6 Connecting and disconnecting the internal source exhaust valve assembly KAD 94 D 6 1 Connecting the internal source exhaust valve 94 D 6 2 Disconnecting the internal source exhaust valve 97 D 7 Source exhaust tubing ...

Page 15: ...122 E 2 4 Configuring the diverter valve waste tubing 125 E 3 Operating the ACQUITY Diverter Valve 126 E 3 1 Programming the diverter valve for operation 126 E 3 2 Manually operating the diverter valve 127 E 3 3 Injecting sample and manually actuating the diverter valve 128 E 4 Diverter valve maintenance procedures 129 E 4 1 Cleaning the diverter valve 129 E 4 2 Replacing the diverter valve compon...

Page 16: ...ortant Do not open the instrument s front door during data acquisition Doing so cancels the acquisition 1 2 Ion optics Ions flow through the instrument in the following sequence 1 Samples from the LC are introduced into the ionization source 2 The ions pass through the sample cone into the vacuum system 3 The ions pass through the transfer optics the ion guides to the quadrupole where they are fil...

Page 17: ...on Where possible use the 250 mm PEEK tubing Doing so minimizes band broadening Notice To avoid the pressure exceeding the limits of the preceding instrument ensure that you use the recommended tubing size and lengths for your instrument 1 5 Vacuum system An internal turbomolecular pump and external backing pump create the instrument s vacuum The Performance instrument uses a separate rotary vane ...

Page 18: ...attached Internal valve Standard Rotary external KAB Performance Diaphragm attached External valve assembly Standard Rotary external Figure 1 1 QDa with internal source exhaust KAD version QDa with internal source exhaust KAD version Maintenance Procedures External Connections June 15 2017 715003956 Rev F Page 18 ...

Page 19: ...Diverter Valve The ACQUITY Diverter Valve is supplied as an optional accessory to the ACQUITY QDa Detector The diverter valve is used to divert column eluent from the QDa to waste when handling variable samples and solutions and can also support sample loop injections You can attach the diverter valve to the QDa instrument using the bracket assembly supplied with the diverter valve kit See also Op...

Page 20: ... in the United States and HAR type power cords or better in Europe For requirements elsewhere contact your local Waters distributor Inspect the power cords for damage and replace them if necessary Power off and unplug each module before performing any maintenance operation on it Connect each module to a common ground To connect to the electricity source Recommendation Use a line conditioner and un...

Page 21: ... place it on a bench top or other surface so that you can exercise caution when inserting the probe see Removing the source enclosure from the instrument Before starting the instrument connect the LC flow to the probe To connect the probe 1 Connect the LC flow or syringe pump to the probe assembly s input and secure the PEEK fitting Warning To avoid puncture injuries from the sharp probe capillary...

Page 22: ...Starting the instrument Warning To avoid injury from electrical shock or fire and damage to the equipment follow these guidelines Do not expose the workstation or ancillary equipment to dripping or splashing liquids Do not place objects filled with liquid such as solvent bottles on top of the workstation or ancillary equipment June 15 2017 715003956 Rev F Page 22 ...

Page 23: ... top left hand sides of the ACQUITY system modules including the QDa detector Result Each module establishes communications with the workstation 4 Allow approximately five minutes for the instruments to establish communications Tip The power and status LEDs show steady green when the instruments have established communications 5 On the workstation start the software Result The instrument performs ...

Page 24: ...trument is removed from the solvent flow path Notice To avoid irreparable damage to the instrument when it is in Standby mode or switched off disconnect the instrument from the solvent flow path If the instrument is connected to the LC via a diverter valve ensure that the diverter valve is set to flow to waste June 15 2017 715003956 Rev F Page 24 ...

Page 25: ...trument exhaust line Check daily empty as required See Emptying the nitrogen exhaust trap Empty the liquid trap bottle in the backing pump exhaust line Check daily empty as required See Emptying the liquid trap bottle Gas ballast the rotary backing pump Performance QDa only ESI weekly See Gas ballasting the rotary backing pump Inspect and adjust the rotary backing pump s oil level Performance QDa ...

Page 26: ...lean the diverter valve or replace its components Clean the valve when performance reduces to unacceptable levels and replace components if they become irretrievably damaged See Diverter valve maintenance procedures 3 2 Spare parts To ensure that your system operates as designed use only Waters Quality Parts Visit www waters com wqp for information about Waters Quality Parts including how to order...

Page 27: ...tively use Remote Desktop a real time collaboration option that controls the two way connection with the system by enabling the Connections INSIGHT iAssist service level These sources available on the Waters Web site provide more information about Connections INSIGHT and Connections INSIGHT iAssist Connections INSIGHT Quick Start Guide Connections INSIGHT User s Guide 3 5 Submitting a Connections ...

Page 28: ...first put the instrument in Standby mode Warning To avoid burn injuries take great care while working with the probe and source these components can be hot Warning To avoid injury ensure that these criteria are met when performing maintenance operations inside the source enclosure The instrument is in Standby mode LC flow is diverted to waste or set to Off Desolvation gas flow is stopped See Appen...

Page 29: ...sconnect the source enclosure s electrical cable from the front of the instrument by loosening the screws and pull the cable from the socket 5 Loosen the 2 thumbscrews on the front of the source enclosure Warning To avoid burn injuries exercise care when handling the components of the source enclosure heated to high temperatures Wait until the hot components have sufficiently cooled before you han...

Page 30: ... personal contamination with biohazardous or toxic compounds wear clean chemical resistant powder free gloves when performing this procedure To fit the source enclosure 1 Use both hands to slide the source enclosure onto the instrument s supporting rods 2 To secure the enclosure against the instrument tighten the 2 thumbscrews on the front of the source enclosure Notice To avoid damage to the elec...

Page 31: ...istant powder free gloves Warning To avoid personal contamination with toxic materials wear clean chemical resistant powder free gloves when performing this procedure Warning To avoid burn injuries allow the ion block to cool before performing this procedure To remove the source components from the ion block 1 Remove the source enclosure see Removing the source enclosure from the instrument 2 Remo...

Page 32: ...cone a disc aperture assembly and a metal entrance aperture carrier The disc aperture assembly contains a metal disc If the disc becomes separated from the rubber carrier simply reinsert the disc into the carrier Requirement The Standard and Performance QDa detectors require different entrance aperture discs When ordering a replacement entrance aperture disc and seal ensure that the entrance apert...

Page 33: ...Figure 3 3 Removing the sample cone assembly Calibration pin Sample cone assembly Ion block Figure 3 4 Sample cone assembly components June 15 2017 715003956 Rev F Page 33 ...

Page 34: ...when performing this procedure To replace the entrance aperture seal 1 Dispose of the old entrance aperture seal and disc according to your local environmental regulations 2 Insert the new entrance aperture seal and disc into the sample cone Requirement The Standard and Performance QDa Detectors require different entrance aperture discs When ordering a replacement entrance aperture disc and seal e...

Page 35: ...de screwdriver beneath the top of the calibration pin and then lever the pin away from the ion block Tip To locate the calibration pin see the image Removing the sample cone assembly in Removing the sample cone assembly from the ion block 2 Remove the sample cone assembly from the right hand side of the ion block see Removing the sample cone assembly from the ion block 3 Remove the 2 screws securi...

Page 36: ...t to break the seal between the ion block and the source housing Figure 3 6 Removing the ion block Slot 2 5 mm hex wrench Leverage path 5 Remove the ion block from the source housing 3 8 4 Cleaning the source components Required materials Chemical resistant powder free gloves Appropriately sized glass vessels in which to completely immerse components when cleaning Use only glassware not previously...

Page 37: ...g To avoid injury when working with formic acid which is extremely corrosive and toxic take extreme care handling it and use a fume hood and suitable protective equipment To maintain the source components 1 Remove any O rings from the source components and replace the calibration pin at the top of the ion block Important If the O ring is visibly damaged replace it Figure 3 7 Ion block and calibrat...

Page 38: ...nic bath for 10 minutes Notice To avoid recontaminating the components wear clean chemical resistant powder free gloves 5 Carefully remove the components from the vessels and blow dry them with inert oil free gas 6 Inspect each component for persisting contamination If contamination is present do as follows a Use the wash bottle containing 1 1 methanol water to rinse the component over the large b...

Page 39: ... them using the 2 5 mm hex wrench 8 Refit the source enclosure see Fitting the source enclosure to the instrument 3 9 Cleaning the instrument case Notice To avoid abrading the surfaces of the instrument do not use abrasives or solvents when cleaning them Use a soft cloth dampened with water to clean the outside surfaces of the mass spectrometer 3 10 Cleaning the ion guide assembly Clean the ion gu...

Page 40: ...ficiently cooled before you handle them Notice To avoid damaging the StepWave ion guide assembly handle it and its components carefully throughout the cleaning procedure In particular do not touch the wiring To remove the ion guide assembly from the source assembly 1 Remove the source enclosure from the instrument see Removing the source enclosure from the instrument 2 Use the 3 mm hex wrench to r...

Page 41: ... Removing the differential aperture in step 5 of this topic Recommendation Clean the differential aperture see Cleaning the differential aperture before disassembling the ion guide housing If cleaning the differential aperture fails to increase signal sensitivity continue with this procedure and then clean the ion guide see Cleaning the ion guide assembly 5 If the differential aperture O ring is v...

Page 42: ...rods Differential aperture support Differential aperture support screws O ring Differential aperture securing screws Differential aperture Ion guide 6 Disconnect the 4 electrical connectors from the ion guide leaving them attached to the pumping block June 15 2017 715003956 Rev F Page 42 ...

Page 43: ...on guide support rods using the 3 mm hex wrench and then remove the differential aperture support and ion guide from the pumping block Note The ion guide is attached to the differential aperture support 8 To detach the ion guide from the differential aperture support remove the 4 ion guide securing screws using the 2 5 mm hex wrench Figure 3 11 Removing the ion guide June 15 2017 715003956 Rev F P...

Page 44: ...ture in the glass vessel 2 Add Waters MS Cleaning Solution or 1 1 methanol water to the vessel until the differential aperture is immersed completely 3 Place the vessel containing the differential aperture in the ultrasonic bath for 20 minutes 4 Carefully pour the cleaning solution from the vessel holding the differential aperture into the holding container retaining the differential aperture in t...

Page 45: ... grade or better 1 1 methanol water Holding container for used Waters MS Cleaning Solution HPLC grade isopropyl alcohol Ultrasonic bath Source of oil free inert gas for example nitrogen for drying Warning To avoid personal contamination with biohazardous or toxic compounds wear clean chemical resistant powder free gloves when performing this procedure To clean the ion guide assembly 1 Bend a PEEK ...

Page 46: ...ning Solution for one subsequent cleaning 5 Fill the vessel with deionized water to rinse the ion guide and then discard the water 6 Refill the vessel with deionized water to rinse the ion guide a second time and then discard the water 7 Fill the vessel with isopropyl alcohol ensuring that the ion guide is immersed completely 8 Place the vessel containing the ion guide in the ultrasonic bath for 2...

Page 47: ...urce assembly 2 Fit the differential aperture to the differential aperture support using the 3 slotted screws and then secure the screws using a flat blade screwdriver 3 Carefully slide the ion guide s PCBs into the differential aperture support 4 Secure the ion guide to the differential aperture support using the four ion guide securing screws Figure 3 13 Assembling the ion guide housing Ion guid...

Page 48: ...ip To correctly orientate the pumping block ensure that the ion guide remains visible at the left hand edge of the pumping block s front face Figure 3 14 Orientating the pumping block Ion guide Pumping block Alignment marks 7 Secure the pumping block to the instrument using the 4 pumping block securing screws and then tighten them using the 2 5 mm hex wrench June 15 2017 715003956 Rev F Page 48 ...

Page 49: ... source components 9 Fit the source enclosure to the instrument see Fitting the source enclosure to the instrument 3 11 Emptying the nitrogen exhaust trap Inspect the nitrogen exhaust trap in the instrument s exhaust line daily and empty it before it is more than 10 full June 15 2017 715003956 Rev F Page 49 ...

Page 50: ...trap Requirement Wear clean chemical resistant powder free gloves when performing this procedure 1 In the software stop the LC flow 2 Remove the source enclosure see Removing the source enclosure from the instrument Warning To avoid personal contamination with biohazardous or toxic compounds wear clean chemical resistant powder free gloves when performing this procedure June 15 2017 715003956 Rev ...

Page 51: ...oil Inspect the VACUUBRAND RE 6 rotary vane pump daily before using it and empty the liquid trap bottle For details about performing this task see the VACUUBRAND Technology for Vacuum Systems Instructions for use VACUUBRAND manual number 999139 11 20 2013 3 13 Maintaining the rotary backing pump s oil Note This section applies to the Performance QDa detector only The Standard instrument uses a dia...

Page 52: ...burn warning symbol To add oil to the backing pump 1 Power off the instrument using the power button in the top left hand corner of the front panel 2 Wait approximately 5 minutes to allow the instrument to vent 3 Switch off the pump and disconnect the power cable 4 Place the pump on a tray suitable for catching dripping oil 5 Remove the oil drain plug from the pump s rear panel Figure 3 16 Backing...

Page 53: ...n plug into the pump s rear panel 3 14 Gas ballasting the rotary backing pump Note This section applies to the Performance QDa detector only The Standard instrument uses a diaphragm pump which does not require oil Notice To avoid damaging the pump do not leave it constantly in ballast mode Doing so can run the pump dry and fill the trap bottle and exhaust lines with oil Note To prevent the pump fa...

Page 54: ...r causes of failure have been investigated Note The following procedures apply only to instruments fitted with the internal source exhaust valve serial number suffix KAD shown in the figure below See Identifying your instrument version for guidance on identifying your QDa version and Connecting the external source exhaust valve assembly KAB for details about replacing the external source exhaust v...

Page 55: ...tubing is in a rear configuration disconnect the source exhaust tubing and rotate the elbow connector to clear the path for removing the source exhaust valve from the enclosure 2 Unscrew and remove the 4 retaining screws from the valve grip panel using the 5 mm hex wrench 3 Hold the grip panel and pull to slide the valve out of the enclosure Tip If the source exhaust valve does not immediately rel...

Page 56: ...t tube elbow connector 4 Dispose of the source exhaust valve in accordance with local environmental regulations 3 16 2 Fitting the source exhaust valve Perform the following procedure to fit the source exhaust valve into the enclosure Required materials Chemical resistant powder free gloves 5 mm hex wrench Source exhaust valve 289010426 June 15 2017 715003956 Rev F Page 56 ...

Page 57: ...r of the path 2 Hold the source exhaust valve by the grip panel orienting the curved edge of the panel toward the left 3 Slide the valve into the enclosure and carefully slot the grip panel keyway over the corresponding alignment key at the top rim of the enclosure entrance Figure 3 19 Inserting the source exhaust valve Source valve enclosure Alignment key and grip panel keyway Valve grip panel Re...

Page 58: ...ough the hose brackets alongside the instrument and inserting the tubing into the elbow connector See Connecting the internal source exhaust valve KAD Note Routing the exhaust tubing through the hose brackets positions the tubing at a downward gradient Doing so prevents the pooling of solvents To prevent the pooling of solvents in a front configuration ensure that the source exhaust tubing is posi...

Page 59: ...o liability in cases of injury or property damage resulting from the failure of individuals to comply with any safety precaution when installing repairing or operating any of its instruments or devices The following symbols warn of risks that can arise when you operate or maintain a Waters instrument or device or component of an instrument or device When one of these symbols appears in a manual s ...

Page 60: ...ogical agents that can pose a serious health threat Warning Risk of tipping Warning Risk of explosion Warning Risk of high pressure gas release A 1 1 Specific warnings The following warnings both symbols and text can appear in the user manuals of particular instruments and devices and on labels affixed to them or their component parts A 1 1 1 Burst warning This warning applies to Waters instrument...

Page 61: ...n Operate mode Warning To avoid harmless static like electric shock ensure that the mass spectrometer is in Standby mode before you touch any of its external surfaces that are marked with this high voltage warning symbol A 1 1 3 Mass spectrometer flammable solvents warning 600 kPa This warning applies to mass spectrometers performing an analysis that requires the use of flammable solvents Warning ...

Page 62: ...ndling of such materials appear in the latest edition of the National Research Council s publication Prudent Practices in the Laboratory Handling and Management of Chemical Hazards Observe Good Laboratory Practice GLP at all times particularly when working with hazardous materials and consult the safety representative for your organization regarding its protocols for handling such materials A 2 No...

Page 63: ...ment approuvée par l autorité responsable de la conformité à la réglementation peut annuler le droit de l utilisateur à exploiter l équipement Warnung Jedwede Änderungen oder Modifikationen an dem Gerät ohne die ausdrückliche Genehmigung der für die ordnungsgemäße Funktionstüchtigkeit verantwortlichen Personen kann zum Entzug der Bedienungsbefugnis des Systems führen Avvertenza qualsiasi modifica ...

Page 64: ... à proximité de l instrument Evitez d utiliser des tubes sévèrement déformés ou endommagés Evitez d utiliser des tubes non métalliques avec du tétrahydrofurane THF ou de l acide sulfurique ou nitrique concentré Sachez que le chlorure de méthylène et le diméthylesulfoxyde entraînent le gonflement des tuyaux non métalliques ce qui réduit considérablement leur pression de rupture Warnung Bei der Arbe...

Page 65: ... sometidos a presión El usuario deberá protegerse siempre los ojos cuando trabaje cerca de tubos de polímero sometidos a presión Si hubiera alguna llama las proximidades No se debe trabajar con tubos que se hayan doblado o sometido a altas presiones Es necesario utilizar tubos de metal cuando se trabaje con tetrahidrofurano THF o ácidos nítrico o sulfúrico concentrados Hay que tener en cuenta que ...

Page 66: ... in a manner not specified by the manufacturer the protection provided by the equipment may be impaired Avertissement L utilisateur doit être informé que si le matériel est utilisé d une façon non spécifiée par le fabricant la protection assurée par le matériel risque d être défectueuses Warnung Der Benutzer wird darauf aufmerksam gemacht dass bei unsachgemäßer Verwendung des Gerätes die eingebaut...

Page 67: ... d incendie remplacez toujours les fusibles par d autres du type et de la puissance indiqués sur le panneau à proximité du couvercle de la boite à fusible de l instrument Warnung Zum Schutz gegen Feuer die Sicherungen nur mit Sicherungen ersetzen deren Typ und Nennwert auf den Tafeln neben den Sicherungsabdeckungen des Geräts gedruckt sind Avvertenza per garantire protezione contro gli incendi sos...

Page 68: ...Kapitels Wartungsverfahren angegeben sind Avvertenza per garantire protezione contro gli incendi sostituire i fusibili con altri dello stesso tipo aventi le caratteristiche indicate nel paragrafo Sostituzione dei fusibili del capitolo Procedure di manutenzione Advertencia Para evitar incendios sustituir los fusibles por aquellos del tipo y características indicados en la sección Sustituir fusibles...

Page 69: ...nd Frame or chassis terminal Fuse Functional ground Input Output A 7 2 Handling symbols The following handling symbols and their associated statements can appear on labels affixed to the packaging in which instruments devices and component parts are shipped Symbol Description Keep upright Keep dry Fragile June 15 2017 715003956 Rev F Page 69 ...

Page 70: ...Symbol Description Use no hooks Upper limit of temperature Lower limit of temperature Temperature limitation June 15 2017 715003956 Rev F Page 70 ...

Page 71: ...tions Attribute Standard QDa Performance QDa Height 21 6 cm 21 6 cm Width 37 4 cm 37 4 cm Depth 76 1 cm 64 6 cm Weight 34 kg 29 kg B 2 Environmental specifications The following table lists the environmental specifications for the ACQUITY QDa detector Table B 2 Environmental specifications Attribute Specification Operating temperature performance is specified 15 C to 28 C Safe operating temperatur...

Page 72: ...s that can produce a reduction of dielectric strength or surface resistivity Degree 2 refers only to normally nonconductive pollution Occasionally however expect a temporary conductivity caused by condensation d Moisture Protection Normal IPX0 IPX0 means that no Ingress Protection against any type of dripping or sprayed water exists The X is a placeholder that identifies protection against dust if...

Page 73: ...Table B 4 Input and output specifications Continued continued Attribute Specification Analog Out Maximum output 10 V Minimum output 10 V Output current 10 mA June 15 2017 715003956 Rev F Page 73 ...

Page 74: ...with the instrument Where solvents are used at higher concentrations than specified in the lists it is important that you determine whether their use is compatible with the instrument C 1 Preventing contamination For information on preventing contamination refer to Controlling Contamination in UltraPerformance LC MS and HPLC MS Systems part number 715001307 You can find this document on www waters...

Page 75: ...haust tube elbow connector Waste liquid drip port C 3 1 Items exposed to solvent The items that appear in the following table can be exposed to solvent You must evaluate the compatibility issues if the solvents used in your application differ from the solvents normally used with these items See Solvents used to prepare mobile phases for details about the most common ingredients used to prepare mob...

Page 76: ...st of solvents and chemicals and their associated concentrations shown below presents the levels that have been tested assessed or both for chemical compatibility with the materials of construction It is not a recommendation for optimal electrospray sensitivity Mobile phase component concentration affects electrospray efficiency and sensitivity Keep modifier and buffer concentrations to the lowest...

Page 77: ...ted in the previous table because they decrease sensitivity However they are often required for suitable chromatographic resolution Note At the concentrations specified below the reagents are not expected to adversely affect the performance of the materials shown in the list of items exposed to solvents in Items exposed to solvent However you must keep concentrations to the lowest levels that main...

Page 78: ... topic Items exposed to solvent Table C 4 Solvents used commonly as diluents Solution Concentration Toluene 5 Methyl tert butyl ether MTBE 5 Hexane 5 Dichloromethane 2 Dimethylformamide DMF 5 Dimethyl sulfoxide DMSO 5 Heptane 5 C 4 QDa solvent compatibility KAB Note The solvent compatibility information in this section refers to the KAB version of the QDa detector shown in the following figure Fig...

Page 79: ...with these items See Solvents used to prepare mobile phases for details about the ingredients used most commonly to prepare mobile phases Table C 5 Items exposed to solvent KAB Item Material Gas exhaust port Aluminium Gas tubes FEP fluorinated ethylene propylene Ion block Stainless steel Ion block support PEEK polyetheretherketone O rings Viton or PTFE polytetrafluoroethylene encapsulated Viton So...

Page 80: ...vents that are referenced in the lists but used at higher concentrations than specified might also be compatible with the instrument In such cases you must determine the compatibility of the solvents before using them Where solvents are used at higher concentrations than specified in the lists it is important that you determine whether their use is compatible with the instrument Notice For LC MS o...

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Page 82: ... to lift the instrument Notice To avoid damaging system modules contact Waters Technical Service before moving the modules If you must transport a system component or remove it from service contact Waters Technical Service for recommended cleaning flushing and packaging procedures D 1 External wiring and vacuum connections When making connections to the QDa detector s rear panel refer to the figur...

Page 83: ...ble Vacuum pump tubing Nitrogen inlet Diaphragm pump securing screw Diaphragm pump supports Diaphragm pump securing screw Waste bottle solenoid connection Power cable For details of supported system configurations contact Waters Technical Service June 15 2017 715003956 Rev F Page 83 ...

Page 84: ...nstrument using the 2 screws and tighten them using the Phillips cross head screwdriver 3 Connect the length of diaphragm pump vacuum hose in step 2 to the 1 inch OD straight vacuum port on the instrument s rear panel Notice The instrument requires two separate exhaust systems one for nitrogen the other for the backing pump Vent them to atmosphere through separate exhaust lines Oil mist can severe...

Page 85: ... backing pump Note To connect the Standard instrument s backing pump see Connecting the Standard instrument s backing pump Figure D 2 Connecting the backing pump Vacuum hose assembly Exhaust port Power cable June 15 2017 715003956 Rev F Page 85 ...

Page 86: ...installation kit Warning To avoid personal contamination with biohazardous or toxic compounds wear clean chemical resistant powder free gloves when performing this procedure Notice To ensure the correct operation of the backing pump it must be installed within one degree of horizontal Notice The area where the backing pump is located must have an ambient temperature of 15 to 40 ºC 59 to 104 ºF Not...

Page 87: ...m 6 inches Front side minimum clearance is 35 56 cm 14 inches To connect the backing pump 1 Place a suitable PTFE drip tray on the floor within 5 feet of the instrument Requirement The pump must be oriented in a way that allows easy daily access to the gas ballast valve and oil level sight glass 2 Place the pump on the PTFE drip tray June 15 2017 715003956 Rev F Page 87 ...

Page 88: ...es two separate exhaust systems one for nitrogen the other for the backing pump Vent them to atmosphere through separate exhaust lines Oil mist can severely damage the instrument if the nitrogen exhaust line connects with the backing pump exhaust line Your warranty does not cover damage caused by routing exhaust lines incorrectly 6 Route the open end of the exhaust tubing to a suitable exhaust ven...

Page 89: ... 1 Making the electrical connections to the rotary backing pump Figure D 4 Backing pump electrical connections To power source Backing pump connector Pump control cable June 15 2017 715003956 Rev F Page 89 ...

Page 90: ...istant powder free gloves Utility knife Wrench 6 mm PTFE tubing included in the installation kit 6 mm stud Nitrogen regulator To connect the nitrogen gas supply Requirement Wear clean chemical resistant powder free gloves when performing this procedure Notice To avoid gas leaks use the sharp knife to cut the PTFE tubing squarely 1 Use the sharp knife to cut a length of 6 mm PTFE tubing long enough...

Page 91: ...suffix KAB If the model of your QDa supports the internal source exhaust valve assembly serial number suffix KAD see the procedures in Connecting and disconnecting the internal source exhaust valve assembly KAD Note See Identifying your instrument version for guidance on identifying the version of your QDa to determine the appropriate procedures to follow for configuring the valve connections on y...

Page 92: ...ust trap bottle follows a continuous downward gradient without loops or areas of compression Connect the exhaust tubing to either the front or rear source exhaust connection to suit your configuration Tip To determine which configuration you are using see the configuration figures beginning at the topic Connecting the external source exhaust valve assembly KAB and ending at the topic Connecting th...

Page 93: ... the assembly cover is depicted as transparent to reveal the tubing connections Figure D 7 External source exhaust valve assembly front configuration Blanking fitting API gas connection Unused source exhaust connection Holding screw Convoluted liquid waste tubing Exhaust tubing Source exhaust connection June 15 2017 715003956 Rev F Page 93 ...

Page 94: ...nd exhaust tubing from the valve assembly D 6 Connecting and disconnecting the internal source exhaust valve assembly KAD Replace the source exhaust valve annually or when a source pressure test fails and all other causes of failure have been investigated The following procedures apply to instruments fitted with the internal source exhaust valve assembly serial number suffix KAD If the model of yo...

Page 95: ...al source exhaust valve front configuration in step 3 2 Thread the PTFE waste tubing alongside the instrument through the two hose brackets from the back end toward the elbow connector Note Routing the exhaust tubing through the hose brackets positions the tubing at a downward gradient Doing so prevents the pooling of solvents To prevent the pooling of solvents in a front configuration ensure that...

Page 96: ...ow connector Waste liquid drain port Figure D 9 Internal source exhaust valve front configuration Source exhaust tube elbow connector Waste liquid drain port PTFE source exhaust tube and elbow connector ensure a downward gradient Exhaust trap bottle June 15 2017 715003956 Rev F Page 96 ...

Page 97: ...Wear clean chemical resistant powder free gloves when performing this procedure 1 Disconnect the source exhaust PTFE tubing from the 90 degree elbow connector on the source exhaust valve 2 Remove the tubing from the hose brackets alongside the QDa panel if the tubing is in a rear facing configuration 3 Disconnect the drain tubing from the waste liquid drain port D 7 Source exhaust tubing D 7 1 Con...

Page 98: ...bly see Connecting and disconnecting the external source exhaust valve assembly KAB and then thread the tube through the hose brackets running alongside the instrument to ensure a downward gradient Tip The downward gradient ensures that solvent flows freely into the trap bottle 4 Connect the 90 degree elbow to the free end of the PTFE tubing that runs alongside the instrument 5 Connect the second ...

Page 99: ...sure downward gradient Liquid waste container Convoluted liquid waste tubing Source exhaust valve assembly Tip This figure shows a QDa detector fitted with the external source exhaust valve assembly serial number suffix KAB To see the QDa detector fitted with the internal source exhaust valve serial number suffix KAD see the figures Internal source exhaust June 15 2017 715003956 Rev F Page 99 ...

Page 100: ... in Connecting the internal source exhaust valve Figure D 11 Rear configuration with LC stack ACQUITY QDa 90 degree elbow To laboratory exhaust system PTFE tubing Exhaust trap bottle Exhaust solenoid cable PTFE tubing ensure downward gradient June 15 2017 715003956 Rev F Page 100 ...

Page 101: ...tion in Connecting the internal source exhaust valve D 7 2 Alternate configurations Notice To prevent solvent leaks and resultant damage to the instrument ensure that the entire PTFE waste line from the instrument to the exhaust trap bottle follows a continuous downward gradient without loops or areas of compression You can adopt an exhaust configuration presented in this section if it better suit...

Page 102: ...nt exhaust connection Tip This figure shows a QDa detector fitted with the external source exhaust valve assembly serial number suffix KAB To see the QDa detector fitted with the internal source exhaust valve serial number suffix KAD see the figures Internal source exhaust valve rear configuration and Internal source exhaust valve front configuration in Connecting the internal source exhaust valve...

Page 103: ...UITY QDa Source exhaust valve assembly To laboratory exhaust system PTFE tubing Exhaust trap bottle Exhaust solenoid cable Liquid waste container Convoluted liquid waste tubing PTFE tubing ensure downward gradient June 15 2017 715003956 Rev F Page 103 ...

Page 104: ...a detector fitted with the internal source exhaust valve serial number suffix KAD does not connect to the 2 5 L waste container Required materials Chemical resistant powder free gloves Bottle stopper assembly 2 5 L bottle Cable ties To connect to the 2 5 L waste container Requirement Wear clean chemical resistant powder free gloves when performing this procedure 1 Connect the bottle stopper tube a...

Page 105: ... KAB only Route both tubes to the bottle stopper assembly on the 2 5 L waste container 6 mm O D tubing Standard QDa only Note Performance QDa detectors use a separate rotary backing pump not an attached diaphragm pump The 4 mm bulkhead fitting and 4 mm O D drain tubing is only used with KAB models and earlier June 15 2017 715003956 Rev F Page 105 ...

Page 106: ... the figure 2 5 L waste container tubing in step 4 Note The Performance QDa detector uses a rotary backing pump which does not require the 6 mm O D drain tube To minimize unused tubing cut the 3 m length of 6 mm O D tubing close to the waste container 4 Position the bottle in a readily accessible location below the instrument Figure D 15 2 5 L waste container tubing 6 mm O D tubing 3 m hose 4 mm O...

Page 107: ...her for the backing pump Vent them to atmosphere through separate exhaust lines Oil mist can seriously damage the instrument if the nitrogen exhaust line connects with the backing pump exhaust line Your warranty does not cover damage caused by routing exhaust lines incorrectly To connect the nitrogen exhaust line 1 Locate the exhaust trap bottle in an accessible area below the instrument see the f...

Page 108: ...ry exhaust system Note To avoid damaging the instrument power it off when connecting the solenoid cable Figure D 16 Exhaust trap bottle From instrument exhaust connection 12 mm OD To laboratory exhaust port 12 mm OD Exhaust solenoid cable D 8 1 Connecting the exhaust solenoid cable To connect the exhaust solenoid cable 1 Connect the exhaust solenoid cable to the port on the exhaust trap bottle Jun...

Page 109: ...of the instrument see External wiring and vacuum connections D 9 Connecting the workstation Warning To avoid injury from electrical shock or fire and damage to the equipment follow these guidelines Do not expose the workstation or ancillary equipment to dripping or splashing liquids Do not place objects filled with liquid such as solvent bottles on top of the workstation or ancillary equipment Jun...

Page 110: ...4 Connect one end of another shielded network cable to the ACQUITY Ethernet switch box 5 Connect the other end of the cable to the port labeled Instrument LAN on the workstation rear panel Notice Do not connect the instrument s power supply cord until you complete the installation procedures in the previous sections D 9 2 Connecting the instrument to the power source 1 Select the correct power cor...

Page 111: ...les of circuits that are typically SELV include contact closure inputs and outputs for autosamplers and UV RI and fluorescence detector signal outputs for LC MS systems The electrical connections on the rear panel of this mass spectrometer are all SELV Notice To avoid damage to the instrument do not exceed these voltages 30 Vdc to the Analog Out connection 30 Vdc to the Stop Flow Out Inject Start ...

Page 112: ...nt In Allows an external device to start data acquisition Maximum 30 V Switch Out Used to send time based contact closure signals to external devices Maximum 30 V 0 5 A 10 W Requirement To meet the regulatory requirements of immunity from external electrical disturbances install connection covers over the signal connectors To make signal connections 1 Reference the signal connection location from ...

Page 113: ...al connections Connector Signal cable 3 Place the second connection cover over the first cover and snap it into place Figure D 20 Connection cover Signal connector Connection cover June 15 2017 715003956 Rev F Page 113 ...

Page 114: ...D 12 Connecting to the power supply To connect the instrument to the power supply refer to Connecting to the electricity source June 15 2017 715003956 Rev F Page 114 ...

Page 115: ...the valve to the instrument using the supplied mounting bracket Do not remove the mount fitted to the ACQUITY Diverter Valve It is used for attaching the valve to the mounting bracket on the QDa E 2 Installing the ACQUITY Diverter Valve E 2 1 Fitting the diverter valve assembly Required materials Mounting bracket Diverter valve and mount 1 4 inch hex nut socket wrench supplied with the ACQUITY Div...

Page 116: ...hex nuts in the recessed holes of the QDa guide bracket that is located on top of the QDa at the front end of the instrument and remove it Figure E 1 Removing the QDa guide bracket Two 1 4 inch hex nuts QDa front guide bracket Retaining studs Instrument case 5 While facing the front of the QDa detector hold the diverter valve mounting bracket with the drip tray shelf positioned to your right and l...

Page 117: ...studs 6 Screw the hex nuts to the studs using the 1 4 inch hex nut socket wrench 7 Fit the diverter valve to the mounting bracket by aligning the two hooks on the diverter valve mount on the left side of the valve with the corresponding slots on the mounting bracket and lower the device until the hooks are securely in the place June 15 2017 715003956 Rev F Page 117 ...

Page 118: ...or The tubing guide obstructs the placement of the diverter valve Required materials 4 mm hex wrench 4 POZIDRIV screwdriver To remove the QDa tubing guide 1 Stop the flow from the LC and switch off the QDa detector 2 Remove the source enclosure from the instrument see Removing the source enclosure from the instrument 3 Use the 4 mm hex wrench to unscrew the six hex cap retaining bolts securing the...

Page 119: ...h cover Air filter Tubing guide Front panel Six 4 mm hex cap retaining bolts 4 Use the 4 POZIDRIV screwdriver to loosen the two 4 POZIDRIV screws fixing the tubing guide to the instrument case remove the guide and tighten the screws June 15 2017 715003956 Rev F Page 119 ...

Page 120: ...hex wrench to screw the six 4 mm hex cap retaining bolts to secure the front panel to the instrument 6 Fit the source enclosure to the instrument see Fitting the source enclosure to the instrument E 2 2 Diverter valve wiring When making connections to the ACQUITY diverter valve s rear panel refer to the figure Rear panel connections in this topic June 15 2017 715003956 Rev F Page 120 ...

Page 121: ... blade screwdriver To connect the diverter valve to the QDa 1 Attach the contact closure cable to the 9 pin D type socket at the rear of the valve 2 At the other end of the cable connect the signal connectors to the corresponding ports on the signal connection box at the rear of the QDa Note Some of these connectors share their port slot with other instruments and devices in the system that connec...

Page 122: ...let E 2 3 Configuring the diverter valve fluidics The ACQUITY diverter valve can be configured to switch the sample flow direction between the QDa and waste in divert mode or configured in loop injection mode Warning To avoid electric shock ensure that the ACQUITY Diverter Valve is grounded before connecting the ESI probe The diverter valve is grounded by fitting it to the mounting bracket on the ...

Page 123: ...ion of the diverter valve operating in divert mode Valve position 1 ON Flow to QDa Valve position 2 OFF Flow to waste To waste blue PEEK tubing 0 01 inch internal diameter From LC red PEEK tubing 0 005 inch internal diameter To QDa red probe assembly 250 mm Not used plugged port Not used plugged port Not used plugged port June 15 2017 715003956 Rev F Page 123 ...

Page 124: ...erations Operating the ACQUITY diverter valve E 2 3 1 Diverter valve tubing considerations Where an optical detector such as a PDA or TUV is configured in line with the ACQUITY QDa Detector and an ACQUITY Diverter Valve it is important to consider the system flow rate due to the maximum recommended pressure limit of the flow cell Typically the maximum flow rate for the QDa is 2 mL min However the ...

Page 125: ...in Blue PEEK tubing up to 500 mm 0 010 inch I D E 2 4 Configuring the diverter valve waste tubing Required materials Blue PEEK tubing Tygon waste tubing PEEK fitting for the diverter valve Y pieces for teeing in waste tubing PEEK tubing cutter Scissors 1 Attach one end of the Tygon tubing to the spur on the underside of the drip tray Figure E 9 Diverter valve waste tubing connections June 15 2017 ...

Page 126: ...amming the diverter valve for operation You can actuate the diverter valve by manually switching the valve positions using the buttons on the front panel by using method events to program the valve operation or by a combination of manual switching and programmed operation You can configure any combination of ON OFF method events Note Configure the method events in the Events table of the QDa Metho...

Page 127: ...erating the valve using programmed and manual actuation Figure E 10 Diverting flow to waste between 1 minute and 1 5 minutes Notes The events can be configured according to your requirements The example is taken from the ACQUITY QDa Detector ICS version 1 68 E 3 2 Manually operating the diverter valve Manually operate the ACQUITY diverter valve by pressing the front panel buttons June 15 2017 7150...

Page 128: ...nject sample and manually actuate the diverter valve 1 Ensure that the QDa is on and mobile phase is flowing from the configured pump 2 Ensure that the valve is in position 1 Note If the valve is not in position 1 manually actuate it by pressing the Down arrow on the front panel to move the valve from position 2 to position 1 3 Inject sample through the injection port 700000472 in valve port 6 usi...

Page 129: ...pare for the next injection manually actuate the valve by pressing the Down arrow on the front panel to move the valve from position 2 to position 1 See also Manually operating the diverter valve Programming the diverter valve operation E 4 Diverter valve maintenance procedures This section provides the maintenance guidelines and procedures necessary to maintain the device s performance Clean the ...

Page 130: ...bing from the valve 3 Remove the 3 hex bolts securing the valve to the rotor using the 9 64 inch hex wrench 4 Remove the valve stator and rotor seal from the valve assembly Retaining bolts Stator Rotor seal Valve pod spindle Diverter valve pod 5 Sonicate the stator and rotor seal in methanol for 20 minutes 6 Reassemble the valve and then evenly tighten the 3 hex bolts to ensure an adequate seal 7 ...

Page 131: ...r Valve Assembly Kit Rotor seal Stator Stainless steel or Titanium To replace the ACQUITY diverter valve stator and rotor seal 1 Stop the flow from the LC ensure that the QDa detector is in standby mode and disconnect the power cable from the diverter valve 2 Disconnect the fluidics tubing from the stator on the front of the diverter valve 3 Use the 9 16 inch hex wrench to unscrew the 3 retaining ...

Page 132: ...uate seal 8 Inspect the tubing and fittings prior to reconnecting and replace if signs of damage or leaking are visible E 4 2 2 Replacing the diverter valve pod Replace the diverter valve pod if it becomes irretrievably damaged See also Replacing the diverter valve stator and rotor seal Required materials Protective eyewear Chemical resistant powder free gloves Diverter valve pod Stainless steel o...

Page 133: ...nmental regulations 6 Slide the new pod into the cuff on the valve and carefully slot the pod key into the corresponding alignment keyway of the pod cuff on the valve Note Ensure that the ridges on the back end of the spindle align with the corresponding ridges on the spindle lock inside the diverter valve unit June 15 2017 715003956 Rev F Page 133 ...

Page 134: ...crew the pod collar onto the pod cuff on the valve 8 Inspect the tubing and fittings prior to reconnecting and replace these if signs of damage or leaking are visible E 5 Removing the diverter valve assembly Required materials 1 4 inch hex nut socket wrench supplied with the ACQUITY Diverter Valve Assembly Kit June 15 2017 715003956 Rev F Page 134 ...

Page 135: ...stem before de stacking it 3 Disconnect the power cable and the contact closure cable from the sockets at the rear of the valve Figure E 15 Diverter valve rear view cable connections 9 pin D type contact closure cable socket USB socket not used Power cable socket Diverter valve mount 4 Detach the fluidics connections from the front of the diverter valve including the following depending on your va...

Page 136: ... side of the device casing from the slots on the mounting bracket on the instrument case Figure E 16 Removing the diverter valve from the mounting bracket Diverter valve mounting bracket Diverter valve mount Diverter valve Drip tray Drip tray waste spur 6 Use the 1 4 inch hex nut socket wrench to unscrew the retaining nuts in the recessed holes on the diverter valve mounting bracket on the top of ...

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