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General
In-line solution heating is a simple and effective
method of providing warmed perfusion solutions to a
bath.
The removable, minimal dead space manifold at the
heater output allows the
SHM Series Multi-line
Solution Heaters
to be used in applications where
from 2 to 8 perfusion lines are connected to a
chamber or other device.
SHM Series
Solution Heaters
are designed for use
with the Warner
TC-324
and
TC-344
Heater
Controllers (all models). The compact design of the
heater makes it possible to install the device
immediately adjacent to the input port of a perfusion
chamber insuring minimum heat loss. Heaters can
accommodate flow rates up to 5 ml/min.
Each heater is supplied with a
TA-29
thermistor cable
assembly (which allows for monitoring of the actual
bath temperature; T2 output on the heater controller),
a 3-way valve, rubber o-rings and 3 meters of
PE-160
tubing.
Tubing Connections
PE-160
tubing (or any other tubing with a 1/16” (1.5
mm) OD is used to connect
SHM Series
heaters to
both the solution reservoir and the perfusion
chamber.
Connections are made by press fitting the tubing onto
the 18 gauge stainless steel hypodermic tubes. Cap
plugs are supplied to block unused inputs.
Assembly
1.
Attach your solution reservoirs to the input ports
of the inline heater using
PE-160
or equivalent
tubing.
2.
All flow lines within the heater must be fluid-
filled prior to use. A 3-way valve is supplied
to facilitate filling of these lines.
Using a short length of
PE-160
tubing, attach
the 3-way valve to the output end of the heater.
Attach a vacuum line to the side port of the
stopcock.
3.
Sequentially fill all input ports on the device with
fluid.
Begin by filling unused input ports with distilled water.
Block each port using the supplied plugs.
Next fill each solution input port with perfusion solution.
4.
Remove the 3-way valve and connect the output port of
the heater to a perfusion chamber using
PE-160
tubing
or equivalent.
5. Place the outboard tubing from the chamber into a
collection reservoir such as a paper cup.
Use a short length of PE tubing between the heater
output and perfusion chamber to minimize heat loss
during delivery.
6. Connect the power cable from the
SHM Series
heater
to the output terminal of your heater controller.
If using a Warner Heater Controller, connect the
TC-29
thermistor cable assembly to the rear panel input of the
heater channel being used on the controller.
Operation
1. Start the solution flow and set the flow rate to the
desired level.
Typical flow rates are 5 ml/min or less and the heater
can sustain temperatures up to 50°C under these
conditions. Faster flow rates are supported but the
output temperature will not be maintained.
Warner Instruments, Inc.
1125 Dixwell Avenue, Hamden, CT 06514 USA
•
(203) 776-0664
•
(800) 599-4203
•
Fax (203) 776-1278
