Taylor-Wharton LABS 20K Operating And Maintenance Instruction Manual Download | Manualshive

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TW-357 REV E

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LABS

(Laboratory Archival Biological Storage)

CRYOSTORAGE SYSTEM

OPERATING AND MAINTENANCE INSTRUCTION

REVIEW AND UNDERSTAND ALL SAFETY PROCEDURES IN FORM # TW-10 P/N 7950-8052
BEFORE ATTEMPTING TO INSTALL, OPERATE OR PERFORM MAINTENANCE ON THIS
CRYOSTORAGE SYSTEM.

DO NOT ATTEMPT TO USE OR MAINTAIN THIS UNIT UNTIL YOU READ AND UNDERSTAND
THESE INSTRUCTIONS. DO NOT PERMIT UNTRAINED PERSONS TO USE OR
MAINTAIN THIS UNIT. IF YOU DO NOT FULLY UNDERSTAND THESE INSTRUCTIONS,
CONTACT YOUR SUPPLIER FOR FURTHER INFORMATION.

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Summary of Contents for LABS 20K

Page 1: ...N FORM TW 10 P N 7950 8052 BEFORE ATTEMPTING TO INSTALL OPERATE OR PERFORM MAINTENANCE ON THIS CRYOSTORAGE SYSTEM DO NOT ATTEMPT TO USE OR MAINTAIN THIS UNIT UNTIL YOU READ AND UNDERSTAND THESE INSTRUCTIONS DO NOT PERMIT UNTRAINED PERSONS TO USE OR MAINTAIN THIS UNIT IF YOU DO NOT FULLY UNDERSTAND THESE INSTRUCTIONS CONTACT YOUR SUPPLIER FOR FURTHER INFORMATION ...

Page 2: ...TW 357 REV E 2 ...

Page 3: ...Components 12 Operating Parameters 14 Adding an Inventory Control System 15 Control Operation 20 Control On Battery Backup Version 31 Solenoid Valve 31 INTERFACE SOFTWARE 32 MAINTENANCE 33 Strainer Cleaning Instructions 33 Defrosting Your LAB Series CryoStorage System 33 Cleaning Your Taylor Wharton CryoStorage System 34 Making Adjustments to the LABS KRYOS Sensor Assembly 35 Removing Installing t...

Page 4: ...olorless odorless and tasteless gas cannot be detected by the human senses and will be inhaled normally as if it were air Without adequate ventilation the expanding nitrogen will displace the normal air resulting in a non life supporting atmosphere Liquid Nitrogen System The liquid nitrogen supply pressure at the inlet to the refrigerator should be in the range of 10 psig 0 7bar 69 kPa to 20 psig ...

Page 5: ...pplied with Taylor Wharton approved controllers If other liquid level controllers are used please contact Taylor Wharton before putting the refrigerator into service Freight Damage Precautions Any Freight damage claims are your responsibility Cryostorage systems are delivered to your carrier from Taylor Wharton s dock in new condition When you receive our product you may expect it to be in that sa...

Page 6: ...indicated in ALL CAPS ITALICS Specific Menu Descriptions under a main category are listed in Italics Taylor Wharton LABS CryoStorage Systems are designed for applications where extremely low temperature storage of biological products is required They are also appropriate for industrial or other applications where liquid nitrogen temperatures and high capacity are needed The LABS style refrigerator...

Page 7: ... 16 5 16 5 16 5 Evaporation Rate1 L day 15 0 9 0 8 Weight Empty lb 1550 920 650 kg 703 417 295 Maximum Gross Weight2 lb 3956 2000 1375 kg 1794 907 624 Inventory Control System Specifications No 5 0 x 5 0 Racks3 58 30 14 No Shelves Rack 13 13 13 No 3 0 x 3 0 Racks4 12 8 4 No Shelves Rack 13 13 13 Vial Capacity 2 ml5 79300 41600 19500 Blood Bag Fenwall 4R9951 2100 1204 560 Blood Bag Fenwall 4R9953 1...

Page 8: ...ogenic solenoid valve Standard Control Type Liquid Level Control or Liquid Level Control with Temperature Control Security User Access via 2 digit password Power On Off Password Menu access Password Alarms Audible Visual red LED Alarm description Remote alarm relay Diagnostics Circuit diagnostics at start up Sensor diagnostics from front panel Thermocouple diagnostics from front panel Manual Test ...

Page 9: ...sponsibility of the shipper to assure that the goods are adequately packaged for return to the factory Liquid Nitrogen Supply Connection The package included with the refrigerator includes a strainer The liquid fill hose from a low pressure source of liquid nitrogen must be connected to the inlet through this fitting This liquid nitrogen source must have a shut off valve and may be any portable li...

Page 10: ...ead screws from the old control located underneath the tabletop 7 Withdraw old controller from underneath table top noting how the controller body has been resting on outer upper head 8 Unplug all connectors and wires and set panel aside 9 Disconnect supply hose from solenoid valve 10 Remove old solenoid 11 Remove spiral wire wrap 12 Mark old sensor locations with electrical tape 13 Measure and ma...

Page 11: ...trogen supply pressure at the inlet to the refrigerator should be in the range of 10 psig 0 7 bar 69 kPa to 20 psig 1 4 bar 138 kPa for optimum performance Higher operating pressures will increase transfer losses and create excessive turbulence of the liquid in the refrigerator which can generate false signals to the liquid level controller causing the refrigerator to under fill In liquid phase st...

Page 12: ...pper outer head See figure 2 0 below Main Control Figure 2 0 KRYOS Main Control Sensor Assembly A standard 7 1 thermistor assembly includes the Freeze Guard over fill sensor Optional 4 thermistor or 8 thermistor sensor assemblies can be ordered The 4 thermistor assembly maintains the liquid level between 2 middle sensors The 8 thermistor assembly maintains the liquid level between the high sensor ...

Page 13: ...ystem is supplied with a transformer compatible with common household North American 110VAC For other power outlets contact Taylor Wharton Customer Service These wall transformers have UL approval UL approval for the system as a whole is not required since the control operates on low voltage If your power source differs or is subject to disruption or line surges due to other equipment on line cons...

Page 14: ...le The thermocouple is normally positioned at the top of the storage rack The factory sensor positions will maintain a liquid level within a distance of 2 in 50 8 mm from the bottom of the operating tray Liquid Phase Storage Liquid phase storage is normally utilized when liquid nitrogen temperatures are required to maintain stored product viability and the storage medium is adequate for storage in...

Page 15: ...ored product Keep ICS inserts drawers or boxes and dividers in good repair Replacement inserts and dividers are available from your Taylor Wharton distributor to keep your system as efficient as possible Always wear gloves when handling ICS racks or stored product as they are very cold read the precautions in the Safety section of these instructions and in Taylor Wharton publication TW 10 Handle W...

Page 16: ...RACKS ARE NOT SQUARE ORIENTATION IS IMPORTANT LABS 80K LABS 40K 2ml vial storage ask about other Box types Part Number Racks 13 x 2 x 100 R23K 8C35 Racks 13 x 2 x 25 R10K 9C44 BOXES 100 N 374187 92 Case of 24 Polycarbonate BOXES 25 N 374180 Case of 36 Polycarbonate VIAL CAPACITY Max allowable LABS 80K 58 12 754 156 79300 LABS 40K 30 8 390 104 41600 LABS 20K 14 4 182 52 19500 Figure 5 0 Vial Storag...

Page 17: ...age in Cassette LABS 20K LABS 80K LABS 40K Fenwal 4R9951 Blood Bags ask about other Bag types PART NUMBER CAPACITY FRAMES CASSETTES LABS 80K 2100 300 2100 LABS 40K 1204 172 1204 LABS 20K 560 80 560 Figure 6 0 Fenwal 4R59951 Blood Bag Rack Arrangements ...

Page 18: ...rage in Cassette LABS 20K LABS 80K LABS 40K Fenwal 4R9953 Blood Bags ask about other Bag types PART NUMBER CAPACITY FRAMES CASSETTES LABS 80K 1505 301 1505 LABS 40K 820 164 820 LABS 20K 400 80 400 Figure 7 0 Fenwal 4R59953 Blood Bag Rack Arrangements ...

Page 19: ...in Cassette LABS 20K LABS 80K LABS 40K Fenwal 4R5462 9955 Blood Bags ask about other Bag types PART NUMBER CAPACITY FRAMES CASSETTES LABS 80K 1050 210 1050 LABS 40K 590 118 590 LABS 20K 290 58 290 Figure 8 0 Fenwal 4R5462 9955 Blood Bag Rack Arrangements ...

Page 20: ...OP button at anytime during the fill Normal Fill Cycle When the refrigerator is filled and the controller is operating the LOW LEVEL and LOW ALARM sensors are immersed in liquid nitrogen Their resistance values are interpreted by the controller as in liquid At the same time the FULL LEVEL and the HIGH ALARM sensors are above the liquid pool sending the controller an above liquid signal As liquid n...

Page 21: ...sing the appropriate number of your menu choice If more menu choices are available than will fit on 1 screen more than 4 choices in this menu section the left hand soft key button will give the More choice Pressing this button will give the user the additional menu choices A shortcut is available to get to the proper menu choice by pressing the appropriate number button The menu choice need not be...

Page 22: ...igh Temperature Alarm Logging 1 4 2 6 High Temperature Alarm Logging 2 4 3 System Logs 4 3 1 Fill Logging 4 3 2 Lid Action Logging 4 3 3 User Access Logs 4 4 Temperature Logs 4 4 1 Thermocouple 1 Log Rate 4 4 2 Thermocouple 2 Log Rate 4 5 Erase Logs 4 5 1 Erase System Logs 4 5 2 Erase Error Logs 4 5 3 Erase Temperature Log 1 4 5 4 Erase Temperature Log 2 5 Security 5 1 Power On Password 5 2 Menu P...

Page 23: ...menu system MENU 1 3 This check will tell if the thermocouples are working or if they are open broken or unplugged If a thermocouple is not connected to the control it will check as open If a thermocouple is Disabled through the menu system it will not show up on the check Level Sensing The level sensing in the system is determined through the use of thermistor based sensor assemblies Thermistors ...

Page 24: ...cur they cause an audible beep as well as a flashing red light A remote alarm relay is also triggered following a user designated period of time after the error condition occurs if it is not corrected In addition the error condition is displayed on the top line until the error condition is corrected When an error does occur the audible alarm may be muted by pressing the designated button The audib...

Page 25: ...ystem MENU 3 3 5 This alarm is activated if either thermocouple experiences an open circuit The possible choices are ENABLE or DISABLE Test Alarms The audible visual and remote alarms can be tested at any time by the user through the menu system MENU 3 4 Follow the instructions on the display to hear the audible chirping indicator of an alarm or to see the red LED flash or to trigger an immediate ...

Page 26: ...m logs out the serial port of the freezer When this option is chosen the display reports how many system logs are stored While the data is being sent to the serial port it can be paused or completely cancelled through the menu system DUMP ERROR LOGS is accessible through the menu system of the control MENU 4 1 2 This option sends data from the error logs to the serial port of the freezer When this...

Page 27: ... the menu system of the control MENU 4 3 3 This menu choice turns on off the logging of user access codes which are requested when the lid is opened Records data in the system log Temperature Logs Temperature Logging Rates for thermocouple 1 T C 1 LOGGING and thermocouple 2 T C 2 LOGGING are accessible through the menu system of the control MENU 4 4 1 or MENU 4 4 2 This menu choice adjusts the rat...

Page 28: ...itch If the lid switch is deactivated this feature will be disabled The choices for auto defog time are disabled through 90 seconds Quick Chill The QUICK CHILL MENU 6 2 2 4 feature provides a burst of N2 gas to the freezer to lower the temperature each time the lid has been opened and then closed This is activated through the lid switch If the lid switch is deactivated this feature will be disable...

Page 29: ...r a printer or a modem System logs error logs or temperature logs are always available for download In addition to downloading data the control can accept commands through the serial port Control settings can be viewed or changed at any time Also remote diagnostics can be performed If the RS 232 is enabled the handshaking can be turned on and off This feature allows two devices to communicate when...

Page 30: ... close Valve Monitoring If KRYOS detects that a flow of LN2 is occurring even when the solenoid valve is supposedly closed an error message will appear on the screen indicating Valve Stuck Open To confirm the control waits for 10 seconds after it determines that the valve should be closed before it will indicate that the valve is stuck open Valve Open Duration Valve Open Duration can be accessed t...

Page 31: ...ion Cable None Connects battery to control board Software V4 0 V4 0 Control Board 1 Black 2 pin solenoid valve plug Red 2 pin solenoid valve plug Red 3 pin battery plug 1 The control board for the battery backup is quite different than the control board for the regular Kryos in that a number of parts are either removed or changed The best way to tell if the control is a battery backed unit or a re...

Page 32: ...nel of the Kryos Even though the control is turned off the battery will still be recharged Check all connections to the Kryos especially the harness plug the wall transformer and the battery extension cable The wall transformer should be plugged into the wall Wait for 30 60 minutes After this time period the battery should have enough power to run the control and continue charging the battery Batt...

Page 33: ...is not successful in 2 minutes the refrigerator should be allowed to thaw to room temperature and purge with dry nitrogen to remove all traces of moisture within the storage chamber before re filling Excessive ice and frost buildup may occur on the refrigerator lid if the lid is left open or the liquid level is too close to the underside of the lid To defrost the lid open the lid to the fully open...

Page 34: ...re described above To clean and sterilize your defrosted LABS CryoStorage System Spray the entire inner vessel surface with ample amounts of an approved disinfectant Allow surface contact to be maintained for a minimum of five minutes Rinse the inner vessel with water remove all water and debris through the access opening located in the carousels bottom tray and vacuum or towel dry the surface Spr...

Page 35: ...ut from the top of the tube and may cause personal injury 3 Close and lock the lid of the LABS CryoStorage System for forty eight 48 hours 4 Open the LABS CryoStorage System and measure the liquid nitrogen level Typically liquid nitrogen levels will drop approximately 3 8 in 9 53 mm per day If your measurement indicates a drop in excess of 3 4 in 19 mm per day please contact your Taylor Wharton di...

Page 36: ... 4 0 5 0 in Thermistor 5 covered Kryos will display 5 0 6 0 in Thermistor 6 covered Kryos will display 6 0 7 0 in Thermistor 7 covered Kryos will display 7 0 in Other Changes The offset has been removed in this version of software to handle the non linearity of the sensor when installed in the refrigerator Metric readings have been removed in this version of the software Recommendations for a Liqu...

Page 37: ...controller is removed from the refrigerator for service the liquid refrigerant level must be maintained manually to protect stored product The fill solenoid valve will be inoperative with the controller removed A flexible fill line terminated with a phase separator may be used periodically through the open refrigerator lid to conduct manual fill operations until automatic operation is restored Sen...

Page 38: ...TW 357 REV E 38 Sensor Plug Schematic Figure 9 0 Pin View Schematics ...

Page 39: ... 39 LABS Plumbing Assembly Solenoid Valve Freeze Guard Sensor Male Branch Tee 90 deg Elbow 90 deg Elbow Male Branch Tee Reducer Relief Valve Tube Strainer Female Connector Relief Valve Figure 10 0 Plumbing Assembly ...

Page 40: ...ce 2 Liquid level is sensed by thermistors located in a sensor tube If the sensor tube is blocked or iced at the top the liquid level in the sensor tube may not rise and fall at the same rate as the liquid level in the freezing chamber Make sure the sensor tube is not obstructed 3 The pool of LN2 can become turbulent during a fill Bubbling and splashing can be amplified by the rack arrangement The...

Page 41: ...st always needed on an LN2 pipeline whether the pipe is Vacuum Jacketed or Foam Insulated 3 Confirm that the solenoid valve is open when a fill is called for If no flow is detected the solenoid valve is not getting the signal to open or it is opening and there is a blockage in the line Check the connections on the leads near the solenoid itself as well as the connector at the controller Confirm th...

Page 42: ...Makes Humming Noise Turn fill valve on and off using the control panel If humming continues it should continue to operate reliably If the noise is excessive replace solenoid valve Transformer Makes Humming Noise This noise is a normal byproduct of most block transformers The transformer has a non renewable internal fuse to protect the transformer output If the noise is excessive replace the transf...

Page 43: ...e inch above the floor i e the first thermistor is offset from the floor zero inches If you would like to stop the fill at a depth greater than seven inches from the floor the sensor assembly must physically be raised inside the sensor tube The distance that it has been raised becomes the new OFFSET and the controller must be notified of this offset from the floor The factory settings are START FI...

Page 44: ... FILL Sensor 5 inches Sensor Assembly Freeze Guard System RS 232 Enabled Handshaking CTS RTS Baud Rate 19200 Date Time USA Central Time Brightness 100 Lid Switch Enabled Auto Defog Timer 20 seconds Quick chill 15 seconds Temperature Control Temperature Control Disabled Temperature ºC Temperature Range ºC Alarms LN2 Supply Alarm 30 minutes Sensor Error Enabled Remote Alarm 30 minutes Lid Open Too L...

Page 45: ...Battery Backup 5140 1158 Freeze Guard Sensor Assembly 5140 1161 Sensor Assembly 7 Sensor 5140 1163 8 Thermistor option 5140 1164 4 Thermistor option 5140 1162 Remote Alarm Plug 5140 1160 Transformer 24VAC R08K 9C04 80K 40K Transformer 16 5VAC 5140 1170 Battery 12 Volt 18Amp Hour 5140 1185 Battery Cable 5140 1182 Thermocouple Assembly R08K 9C51 Lid Switch 5140 1195 Plumbing Assy 16 5 VAC LA80 8C65 ...

Page 46: ..._ Off Note Gasket condition ____ Seals ____ Leaks Audible Alarm ____ On ____ Off Display Lights ____ On ____ Off Temp control setpoint ______ degrees Taylor Wharton Technical Services Phone 800 898 2657 Fax 251 408 2209 Service History Log note date and log number on each service entry Date _________________________________________________________________________________________ Date _____________...

Page 47: ...47 4075 Hamilton Blvd Theodore Alabama 36582 U S A Telephone 251 443 8680 Fax 251 443 2250 In U S and Canada 800 TW TANKS 898 2657 2002 Harsco Corporation ...

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