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BX53/BX53M-P

This instruction manual is for the Olympus Polarizing Microscope. To obtain optimum performance and 
to familiarize yourself fully with the use of your microscope, we recommend that you read this manual 
together with the instruction manual for the BX53/BX53M microscope thoroughly before use. Retain this 
instruction manual in an easily accessible place near the work desk for future reference.

INSTRUCTIONS

POLARIZING MICROSCOPE

A X 7 9 8 8

Optical Microscope and Accessory

Summary of Contents for BX53M-P

Page 1: ...with the use of your microscope we recommend that you read this manual together with the instruction manual for the BX53 BX53M microscope thoroughly before use Retain this instruction manual in an ea...

Page 2: ......

Page 3: ...4 2 Intermediate Polarizing Attachment 15 5 POLARIZED LIGHT OBSERVATION 16 5 1 Adjustments Before Observation 16 1 Adjusting the Optical Axis 16 2 Adjusting for Extinction 22 3 Adjusting the Eyepiece...

Page 4: ...TION 1 Getting Ready 1 A microscope is a precision instrument Handle it with care and avoid subjecting it to sudden or severe impact 2 The BX53 BX53M microscope can be used with up to two additional i...

Page 5: ...ight observation Strain free objective series Strain free condenser BX53 BX53M Microscope frame Observation tube Centerable revolving nosepiece Polarizing intermediate attachment Eyepiece 1 1 NOMENCLA...

Page 6: ...ng any parts or touching glass surfaces CAUTION Light source unit Eyepiece Centerable revolving nosepiece U P4RE Test plate adapter U TAD Stage clips Mechanical stage U FMP Polarizing rotatable stage...

Page 7: ...age bracket with the positioning pin on the stage aligned with the groove on the front of the stage bracket then loosely tighten the centering knobs Mounting Stage Clips and Mechanical Stage U FMP Fig...

Page 8: ...limit 3 Loosen the condenser clamping screw 4 Position the condenser with the scale markings in front insert it into the substage fork as far as it will go Align the positioning pin at the back of th...

Page 9: ...reases from low to high power in a clock wise direction from the primary hole Fig 4 Low Fig 5 High 4 Mounting the Revolving Nosepiece U P4RE Fig 5 1 Turn the coarse adjustment knob to lower the stage...

Page 10: ...ening on the microscope frame and clamp by tightening the clamping screw When Using Intermediate Attachment for conoscopic and orthoscopic observation U CPA Always make sure to mount this unit in such...

Page 11: ...intermediate attachment placing the observation tube to point the binocular eyepieces towards the front Fasten the observation tube by tightening the clamping screw Insert the eyepiece with cross line...

Page 12: ...AN360P place the fixed analyzer in the test plate adapter U TAD Position the fixed analyzer so that the positioning pin on the fixed analyzer fits into the groove The fixed analyzer will be held in p...

Page 13: ...nts are introduced on the following pages Bertrand lens knob Slider clamping knob Mechanical stage Aperture iris diaphragm lever Polarizer rotation ring Bertrand lens focusing knob Analyzer Slot for c...

Page 14: ...60P Test Plate Adapter U TAD Sensitive Tint Plate U TP530 Quarter Wave Plate U TP137 Analyzer slot Stopper knob Clamping knob Rotation dial Hole for the fixed analyzer U ANT Test plate clamping knob S...

Page 15: ...e clips When Using the Mechanical Stage U FMP Fig 12 Open the spring loaded finger and place the specimen on the stage Applicable slide glass is for inorganic substances 28 x 48 mm and organic substan...

Page 16: ...top lever located at the right side of the stage is moved toward the observer and the stage is moved from this position to the first click stop position the specimen is moved 45 to its diagonal positi...

Page 17: ...the stage from the microscope See page 4 2 Using the Allen screwdriver loosen the substage bracket clamping screw and remove the substage assembly Fig 15 3 Turn the coarse adjustment knob and raise th...

Page 18: ...ediate Polarizing Attachment 2 Using the Analyzer Fig 18 3 Focusing the Conoscopic Image U CPA only Fig 18 The analyzer is engaged when pushed in to the second click stop position To disengaged the an...

Page 19: ...ig 19 2 Remove the condenser 3 Insert the rotatable analyzer U AN360P into the analyzer slot on the intermediate attachment Engage the analyzer loosen the clamping screw and set the analyzer s rotatio...

Page 20: ...Place the specimen 2 Focus on the specimen and look for an easily recognizable detail in the field Move this detail in the center of the eyepiece cross lines 3 When the stage is rotated the detail mov...

Page 21: ...ght path one after another When Using Intermediate Attachment for conoscopic and orthoscopic observation U CPA Normal Observation Make sure the 10X objective is inserted into the primary hole on the c...

Page 22: ...ing Intermediate Attachment for conoscopic and orthoscopic observation U CPA Photomicrography 1 Using the tip of a mechanical pencial etc push out in the direction of the objective hole the black rubb...

Page 23: ...mage Fig 27 7 Insert the two provided centering wrenches into the centering holes for the primary hole with the 20X or 10X objective on the nosepiece 8 Manipulate the centering wrenches to bring the b...

Page 24: ...will move the specimen detail in the opposite direction away from the cross line center 4 Moving the specimen only move a new specimen detail into the cross line center Repeat steps 3 and 4 several t...

Page 25: ...10X objective 2 Insert the rotatable analyzer into the light path and set vibration direction scale at the 0 position Clamp with the clamping knob Fig 30 3 Set the polarizer scale at the 0 position 4...

Page 26: ...provided with the U CPA or U OPA is required Orientation plate U PJ Single module name Remove the test plate adapter and the compensator from the light path 1 Swing out the top lens of the condenser 2...

Page 27: ...ving rotate the stage to locate the position where the orientation plate is darkest At this point clamp the stage 5 Remove the analyzer from the light path Set to brightfield and slightly loosen the o...

Page 28: ...the Bertrand lens from the light path 2 Insert the analyzer and perform observation When the top lens is swung out the aperture iris diaphragm and field iris diaphragm will not function nor mally If t...

Page 29: ...he aperture iris diaphragm 6 Slide the focusing knob on the U CPA to focus on the conoscopic image When the intermediate attachment conoscopic and orthoscopic observation U CPA is not used conoscopic...

Page 30: ...e analyzer U AN360P 2 Analyzer U AN360P 360 dial rotation Minimum scale reading 0 1 Vernier scale 3 Revolving nosepiece U P4RE Type Centerable quadruple Attachable compensators Quarter wave plate U TP...

Page 31: ...lens swung in Applicable objectives 4X to 100X 4X objective is used with the top lens swung out Operating environment Indoor use Altitude up to 2000 m Temperature 5 to 40 C 41 to 104 F Maximum relati...

Page 32: ...100XO 0 13 0 3 0 5 0 75 1 3 17 0 10 0 2 1 0 51 0 20 0 17 0 17 0 17 2 58 1 12 0 67 0 45 0 26 40X 100X 200X 400X 1000X 83 6 14 7 4 60 1 66 0 43 5 5 2 2 1 1 0 55 0 22 Oil immersion UIS series objectives...

Page 33: ...e light path 15 b Vignetting or uneven field of view Test plate is stopped at an intermediate position Set at click stop position 9 During orthoscopic observation the condenser top lens is placed in t...

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Page 36: ...ter Our Website https www olympus ims com service and support service centers https www olympus ims com This product is manufactured by EVIDENT CORPORATION effective as of Apr 1 2022 Please contact ou...

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